Genomic analysis of Kazachstania aerobia and Kazachstania servazzii reveals duplication of genes related to acetate ester production
Genomic analysis of Kazachstania aerobia and Kazachstania servazzii reveals duplication of genes related to acetate ester production
Kazachstania aerobia and Kazachstania servazzii can affect wine aroma by increasing acetate ester concentrations, most remarkably phenylethyl acetate and isoamyl acetate. The genetic basis of this is unknown, there being little to no sequence data available on the genome architecture. We report for the first time the near-complete genome sequence of the two species using long-read (PacBio) sequencing (K. aerobia 20 contigs, one scaffold; and K. servazzii 22 contigs, one scaffold). The annotated genomes of K. aerobia (12.5 Mb) and K. servazzii (12.3 Mb) were compared to Saccharomyces cerevisiae genomes (laboratory strain S288C and wine strain EC1118). Whilst a comparison of the two Kazachstania spp. genomes revealed few differences between them, divergence was evident in relation to the genes involved in ester biosynthesis, for which gene duplications or absences were apparent. The annotations of these genomes are valuable resources for future research into the evolutionary biology of Kazachstania and other yeast species (comparative genomics) as well as understanding the metabolic processes associated with alcoholic fermentation and the production of secondary 'aromatic' metabolites (transcriptomics, proteomics and metabolomics).
ester genes, Kazachstania aerobia, Kazachstania servazzii, PacBio, whole genome sequencing, wine aroma
Lin, Mandy Man Hsi
3bc71e7e-a7b5-4c1e-b460-9cad27691ca7
Walker, Michelle E.
5e8a98ce-9e08-409a-99e4-a0b96a490940
Jiranek, Vladimir
8e5a8dfd-f5b2-43e3-928b-11dff324abc7
Sumby, Krista M.
f54b8f1c-c29e-488b-ab5e-dbb9b2576ba9
June 2023
Lin, Mandy Man Hsi
3bc71e7e-a7b5-4c1e-b460-9cad27691ca7
Walker, Michelle E.
5e8a98ce-9e08-409a-99e4-a0b96a490940
Jiranek, Vladimir
8e5a8dfd-f5b2-43e3-928b-11dff324abc7
Sumby, Krista M.
f54b8f1c-c29e-488b-ab5e-dbb9b2576ba9
Lin, Mandy Man Hsi, Walker, Michelle E., Jiranek, Vladimir and Sumby, Krista M.
(2023)
Genomic analysis of Kazachstania aerobia and Kazachstania servazzii reveals duplication of genes related to acetate ester production.
Microbial Genomics, 9 (6), [001029].
(doi:10.1099/mgen.0.001029).
Abstract
Kazachstania aerobia and Kazachstania servazzii can affect wine aroma by increasing acetate ester concentrations, most remarkably phenylethyl acetate and isoamyl acetate. The genetic basis of this is unknown, there being little to no sequence data available on the genome architecture. We report for the first time the near-complete genome sequence of the two species using long-read (PacBio) sequencing (K. aerobia 20 contigs, one scaffold; and K. servazzii 22 contigs, one scaffold). The annotated genomes of K. aerobia (12.5 Mb) and K. servazzii (12.3 Mb) were compared to Saccharomyces cerevisiae genomes (laboratory strain S288C and wine strain EC1118). Whilst a comparison of the two Kazachstania spp. genomes revealed few differences between them, divergence was evident in relation to the genes involved in ester biosynthesis, for which gene duplications or absences were apparent. The annotations of these genomes are valuable resources for future research into the evolutionary biology of Kazachstania and other yeast species (comparative genomics) as well as understanding the metabolic processes associated with alcoholic fermentation and the production of secondary 'aromatic' metabolites (transcriptomics, proteomics and metabolomics).
Text
mgen001029
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Accepted/In Press date: 6 April 2023
e-pub ahead of print date: 5 June 2023
Published date: June 2023
Additional Information:
Funding Information:
This project was supported by funding from Wine Australia in partnership with the University of Adelaide [UA 1803_2.1] and funding from The Australian Research Council Training Centre for Innovative Wine Production [www.ARCwinecentre.org.au; project number IC70100008], which is funded by the Australian Government with additional support from Wine Australia and industry partners. M.L. is a University of Adelaide Divisional Scholarship Holder. The University of Adelaide and CSIRO are members of the Wine Innovation Cluster (http://www.thewaite.org.waite-partners/wine-innovation-cluster/).
Publisher Copyright:
© 2023 The Authors.
Keywords:
ester genes, Kazachstania aerobia, Kazachstania servazzii, PacBio, whole genome sequencing, wine aroma
Identifiers
Local EPrints ID: 482124
URI: http://eprints.soton.ac.uk/id/eprint/482124
ISSN: 2057-5858
PURE UUID: 7b4726b3-0c15-45c4-bf67-31f686a21fa8
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Date deposited: 19 Sep 2023 17:06
Last modified: 18 Mar 2024 04:12
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Contributors
Author:
Mandy Man Hsi Lin
Author:
Michelle E. Walker
Author:
Vladimir Jiranek
Author:
Krista M. Sumby
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