Vimentin protects differentiating stem cells from stress
Vimentin protects differentiating stem cells from stress
Vimentin is one of the first cytoplasmic intermediate filaments to be expressed in mammalian cells during embryogenesis, but its role in cellular fitness has long been a mystery. Vimentin is acknowledged to play a role in cell stiffness, cell motility, and cytoplasmic organization, yet it is widely considered to be dispensable for cellular function and organismal development. Here, we show that Vimentin plays a role in cellular stress response in differentiating cells, by recruiting aggregates, stress granules, and RNA-binding proteins, directing their elimination and asymmetric partitioning. In the absence of Vimentin, pluripotent embryonic stem cells fail to differentiate properly, with a pronounced deficiency in neuronal differentiation. Our results uncover a novel function for Vimentin, with important implications for development, tissue homeostasis, and in particular, stress response.
Animals, Cell Differentiation/physiology, Cells, Cultured, DNA Helicases/metabolism, Fibroblasts/cytology, Mice, Mice, Knockout, Mouse Embryonic Stem Cells/cytology, Neurons/cytology, Poly-ADP-Ribose Binding Proteins/metabolism, Protein Aggregates/physiology, RNA Helicases/metabolism, RNA Recognition Motif Proteins/metabolism, RNA-Binding Proteins/metabolism, Stress, Physiological, Vimentin/genetics
Pattabiraman, Sundararaghavan
a9f5aac6-6388-4b65-ba29-33aa47aad5a4
Azad, Gajendra Kumar
50ceff7e-3b5d-4dd8-9200-5f900aa9d1ba
Amen, Triana
388dc540-e819-4d07-8f1e-ee0f3949a54b
Brielle, Shlomi
580d39ee-c7f1-4cf0-84be-2967ee240e13
Park, Jung Eun
e87ac9f1-a77c-495a-8ba4-1cdc7269881e
Sze, Siu Kwan
0d4129d6-36e2-4bc0-bb23-ebf10e27f711
Meshorer, Eran
5216c1ea-720e-48f6-8838-e9f01ddd5659
Kaganovich, Daniel
ebb13f4e-e925-4aef-88e7-ddc25ef52d8f
11 November 2020
Pattabiraman, Sundararaghavan
a9f5aac6-6388-4b65-ba29-33aa47aad5a4
Azad, Gajendra Kumar
50ceff7e-3b5d-4dd8-9200-5f900aa9d1ba
Amen, Triana
388dc540-e819-4d07-8f1e-ee0f3949a54b
Brielle, Shlomi
580d39ee-c7f1-4cf0-84be-2967ee240e13
Park, Jung Eun
e87ac9f1-a77c-495a-8ba4-1cdc7269881e
Sze, Siu Kwan
0d4129d6-36e2-4bc0-bb23-ebf10e27f711
Meshorer, Eran
5216c1ea-720e-48f6-8838-e9f01ddd5659
Kaganovich, Daniel
ebb13f4e-e925-4aef-88e7-ddc25ef52d8f
Pattabiraman, Sundararaghavan, Azad, Gajendra Kumar, Amen, Triana, Brielle, Shlomi, Park, Jung Eun, Sze, Siu Kwan, Meshorer, Eran and Kaganovich, Daniel
(2020)
Vimentin protects differentiating stem cells from stress.
Scientific Reports, 10 (1), [19525].
(doi:10.1038/s41598-020-76076-4).
Abstract
Vimentin is one of the first cytoplasmic intermediate filaments to be expressed in mammalian cells during embryogenesis, but its role in cellular fitness has long been a mystery. Vimentin is acknowledged to play a role in cell stiffness, cell motility, and cytoplasmic organization, yet it is widely considered to be dispensable for cellular function and organismal development. Here, we show that Vimentin plays a role in cellular stress response in differentiating cells, by recruiting aggregates, stress granules, and RNA-binding proteins, directing their elimination and asymmetric partitioning. In the absence of Vimentin, pluripotent embryonic stem cells fail to differentiate properly, with a pronounced deficiency in neuronal differentiation. Our results uncover a novel function for Vimentin, with important implications for development, tissue homeostasis, and in particular, stress response.
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More information
Accepted/In Press date: 22 October 2020
Published date: 11 November 2020
Keywords:
Animals, Cell Differentiation/physiology, Cells, Cultured, DNA Helicases/metabolism, Fibroblasts/cytology, Mice, Mice, Knockout, Mouse Embryonic Stem Cells/cytology, Neurons/cytology, Poly-ADP-Ribose Binding Proteins/metabolism, Protein Aggregates/physiology, RNA Helicases/metabolism, RNA Recognition Motif Proteins/metabolism, RNA-Binding Proteins/metabolism, Stress, Physiological, Vimentin/genetics
Identifiers
Local EPrints ID: 482131
URI: http://eprints.soton.ac.uk/id/eprint/482131
ISSN: 2045-2322
PURE UUID: df2c01fa-4d4c-47a2-959b-312750125f4a
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Date deposited: 19 Sep 2023 17:12
Last modified: 17 Mar 2024 04:22
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Contributors
Author:
Sundararaghavan Pattabiraman
Author:
Gajendra Kumar Azad
Author:
Triana Amen
Author:
Shlomi Brielle
Author:
Jung Eun Park
Author:
Siu Kwan Sze
Author:
Eran Meshorer
Author:
Daniel Kaganovich
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