Water is a biomarker of changes in the cellular environment in live animals
Water is a biomarker of changes in the cellular environment in live animals
The biological processes that are associated with the physiological fitness state of a cell comprise a diverse set of molecular events. Reactive oxygen species (ROS), mitochondrial dysfunction, telomere shortening, genomic instability, epigenetic changes, protein aggregation, and down-regulation of quality control mechanisms are all hallmarks of cellular decline. Stress-related and decline-related changes can be assayed, but usually through means that are highly disruptive to living cells and tissues. Biomarkers for organismal decline and aging are urgently needed for diagnostic and drug development. Our goal in this study is to provide a proof-of-concept for a non-invasive assay of global molecular events in the cytoplasm of living animals. We show that Microwave Dielectric Spectroscopy (MDS) can be used to determine the hydration state of the intracellular environment in live C. elegans worms. MDS spectra were correlative with altered states in the cellular protein folding environment known to be associated with previously described mutations in the C. elegans lifespan and stress-response pathways.
Aging/genetics, Animals, Biomarkers/metabolism, Body Water/metabolism, Caenorhabditis elegans/cytology, Cytoplasm/metabolism, Dielectric Spectroscopy, Genomic Instability, Longevity/genetics, Oxidative Stress/genetics, Protein Folding, Proteins/metabolism, Reactive Oxygen Species/metabolism, Telomere Shortening
Siwach, Pratibha
843aa2e1-7d29-4ddd-935b-a3dca67c7a90
Levy, Evgeniya
0bc2b78a-d3ad-4d4b-8bd6-329d3da53677
Livshits, Leonid
03fd8594-188f-4cfd-8fc6-f2ae9b61526d
Feldman, Yuri
44a4aa4a-e0cd-4852-a48d-3223735ff21d
Kaganovich, Daniel
ebb13f4e-e925-4aef-88e7-ddc25ef52d8f
4 June 2020
Siwach, Pratibha
843aa2e1-7d29-4ddd-935b-a3dca67c7a90
Levy, Evgeniya
0bc2b78a-d3ad-4d4b-8bd6-329d3da53677
Livshits, Leonid
03fd8594-188f-4cfd-8fc6-f2ae9b61526d
Feldman, Yuri
44a4aa4a-e0cd-4852-a48d-3223735ff21d
Kaganovich, Daniel
ebb13f4e-e925-4aef-88e7-ddc25ef52d8f
Siwach, Pratibha, Levy, Evgeniya, Livshits, Leonid, Feldman, Yuri and Kaganovich, Daniel
(2020)
Water is a biomarker of changes in the cellular environment in live animals.
Scientific Reports, 10 (1), [9095].
(doi:10.1038/s41598-020-66022-9).
Abstract
The biological processes that are associated with the physiological fitness state of a cell comprise a diverse set of molecular events. Reactive oxygen species (ROS), mitochondrial dysfunction, telomere shortening, genomic instability, epigenetic changes, protein aggregation, and down-regulation of quality control mechanisms are all hallmarks of cellular decline. Stress-related and decline-related changes can be assayed, but usually through means that are highly disruptive to living cells and tissues. Biomarkers for organismal decline and aging are urgently needed for diagnostic and drug development. Our goal in this study is to provide a proof-of-concept for a non-invasive assay of global molecular events in the cytoplasm of living animals. We show that Microwave Dielectric Spectroscopy (MDS) can be used to determine the hydration state of the intracellular environment in live C. elegans worms. MDS spectra were correlative with altered states in the cellular protein folding environment known to be associated with previously described mutations in the C. elegans lifespan and stress-response pathways.
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More information
Accepted/In Press date: 12 May 2020
Published date: 4 June 2020
Keywords:
Aging/genetics, Animals, Biomarkers/metabolism, Body Water/metabolism, Caenorhabditis elegans/cytology, Cytoplasm/metabolism, Dielectric Spectroscopy, Genomic Instability, Longevity/genetics, Oxidative Stress/genetics, Protein Folding, Proteins/metabolism, Reactive Oxygen Species/metabolism, Telomere Shortening
Identifiers
Local EPrints ID: 482132
URI: http://eprints.soton.ac.uk/id/eprint/482132
ISSN: 2045-2322
PURE UUID: 7f88ab7d-2ec7-4640-a2cb-570245114a49
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Date deposited: 19 Sep 2023 17:12
Last modified: 17 Mar 2024 04:17
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Contributors
Author:
Pratibha Siwach
Author:
Evgeniya Levy
Author:
Leonid Livshits
Author:
Yuri Feldman
Author:
Daniel Kaganovich
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