Mechanistic observation of interactions between macrophages and inorganic particles with different densities
Mechanistic observation of interactions between macrophages and inorganic particles with different densities
Many different types of inorganic materials are processed into nano/microparticles for medical utilization. The impact of selected key characteristics of these particles, including size, shape, and surface chemistries, on biological systems, is frequently studied in clinical contexts. However, one of the most important basic characteristics of these particles, their density, is yet to be investigated. When the particles are designed for drug delivery, highly mobile macrophages are the major participants in cellular levels that process them in vivo. As such, it is essential to understand the impact of particles’ densities on the mobility of macrophages. Here, inorganic particles with different densities are applied, and their interactions with macrophages studied. A set of these particles are incubated with the macrophages and the outcomes are explored by optical microscopy. This microscopic view provides the understanding of the mechanistic interactions between particles of different densities and macrophages to conclude that the particles’ density can affect the migratory behaviors of macrophages: the higher the density of particles engulfed inside the macrophages, the less mobile the macrophages become. This work is a strong reminder that the density of particles cannot be neglected when they are designed to be utilized in biological applications.
inorganic particles, macrophages, mechanistic observation, migration, particle density, Particle Size, Humans, Macrophages/ultrastructure
e2204781
Zhang, Chengchen
abc47c06-4b99-4aed-be72-463f211e9dfa
Tang, Jianbo
87473905-8e35-48a1-a776-88080bb751b9
Xie, Wanjie
718129dc-cebc-4007-a495-a791960284d4
Allioux, Francois Marie
06fa4c1f-9d93-4fa4-bff6-2b4b4d497e95
Cao, Zhenbang
2bee3c99-a657-4f91-8a2c-76b301bbab25
Biazik, Joanna M.
22e0202e-d477-40ad-9f7a-edc84bd14978
Tajik, Mohammad
29e71941-6210-47f8-ba5c-bee1e77a4947
Deng, Fei
426fa10e-62cf-4593-b476-160aa39a8ece
Li, Yi
f248da75-23e0-4494-97ec-38ddeb7ec764
Abbasi, Roozbeh
e052784f-2858-4c2d-aff8-2fee6fcd479d
Baharfar, Mahroo
4f5e3122-2402-4c84-8513-cf24eeacf3ff
Mousavi, Maedehsadat
43bca89f-f14f-4c48-95f2-8a3b021945de
Esrafilzadeh, Dorna
5b245c2f-64b0-4d63-8477-ef183d9ea152
Kalantar-Zadeh, Kourosh
aded6a64-8612-40b7-aae9-233fbae916a6
25 January 2023
Zhang, Chengchen
abc47c06-4b99-4aed-be72-463f211e9dfa
Tang, Jianbo
87473905-8e35-48a1-a776-88080bb751b9
Xie, Wanjie
718129dc-cebc-4007-a495-a791960284d4
Allioux, Francois Marie
06fa4c1f-9d93-4fa4-bff6-2b4b4d497e95
Cao, Zhenbang
2bee3c99-a657-4f91-8a2c-76b301bbab25
Biazik, Joanna M.
22e0202e-d477-40ad-9f7a-edc84bd14978
Tajik, Mohammad
29e71941-6210-47f8-ba5c-bee1e77a4947
Deng, Fei
426fa10e-62cf-4593-b476-160aa39a8ece
Li, Yi
f248da75-23e0-4494-97ec-38ddeb7ec764
Abbasi, Roozbeh
e052784f-2858-4c2d-aff8-2fee6fcd479d
Baharfar, Mahroo
4f5e3122-2402-4c84-8513-cf24eeacf3ff
Mousavi, Maedehsadat
43bca89f-f14f-4c48-95f2-8a3b021945de
Esrafilzadeh, Dorna
5b245c2f-64b0-4d63-8477-ef183d9ea152
Kalantar-Zadeh, Kourosh
aded6a64-8612-40b7-aae9-233fbae916a6
Zhang, Chengchen, Tang, Jianbo, Xie, Wanjie, Allioux, Francois Marie, Cao, Zhenbang, Biazik, Joanna M., Tajik, Mohammad, Deng, Fei, Li, Yi, Abbasi, Roozbeh, Baharfar, Mahroo, Mousavi, Maedehsadat, Esrafilzadeh, Dorna and Kalantar-Zadeh, Kourosh
(2023)
Mechanistic observation of interactions between macrophages and inorganic particles with different densities.
Small, 19 (4), , [2204781].
(doi:10.1002/smll.202204781).
Abstract
Many different types of inorganic materials are processed into nano/microparticles for medical utilization. The impact of selected key characteristics of these particles, including size, shape, and surface chemistries, on biological systems, is frequently studied in clinical contexts. However, one of the most important basic characteristics of these particles, their density, is yet to be investigated. When the particles are designed for drug delivery, highly mobile macrophages are the major participants in cellular levels that process them in vivo. As such, it is essential to understand the impact of particles’ densities on the mobility of macrophages. Here, inorganic particles with different densities are applied, and their interactions with macrophages studied. A set of these particles are incubated with the macrophages and the outcomes are explored by optical microscopy. This microscopic view provides the understanding of the mechanistic interactions between particles of different densities and macrophages to conclude that the particles’ density can affect the migratory behaviors of macrophages: the higher the density of particles engulfed inside the macrophages, the less mobile the macrophages become. This work is a strong reminder that the density of particles cannot be neglected when they are designed to be utilized in biological applications.
Text
Small - 2022 - Zhang
- Version of Record
More information
Accepted/In Press date: 8 November 2022
e-pub ahead of print date: 29 November 2022
Published date: 25 January 2023
Additional Information:
Funding Information:
The authors would like to thank the Australian Research Council Laureate Fellowship grant (FL180100053) for the financial support. The authors are also grateful to the Electron Microscope Unit, Katharina Gaus Light Microscopy Facility, Bioanalytical Mass Spectrometry Facility, and Solid State & Elemental Analysis Unit within the Mark Wainwright Analytical Centre at UNSW Sydney for the sample characterization and analysis. Special thanks to the support from the technical staff: Dr. Michael Carnell and Dr. Alex Macmillan for the cell live imaging, Dr. Ling Zhong for the LC-MS/MS analysis, and Ms. Rabeya Akter for the ICP-OES analysis. Open access publishing facilitated by University of New South Wales, as part of the Wiley - University of New South Wales agreement via the Council of Australian University Librarians.
Publisher Copyright:
© 2022 The Authors. Small published by Wiley-VCH GmbH.
Keywords:
inorganic particles, macrophages, mechanistic observation, migration, particle density, Particle Size, Humans, Macrophages/ultrastructure
Identifiers
Local EPrints ID: 482309
URI: http://eprints.soton.ac.uk/id/eprint/482309
ISSN: 1613-6810
PURE UUID: 5e15624e-2107-4463-9a64-243b69a830f4
Catalogue record
Date deposited: 26 Sep 2023 16:50
Last modified: 18 Mar 2024 04:15
Export record
Altmetrics
Contributors
Author:
Chengchen Zhang
Author:
Jianbo Tang
Author:
Wanjie Xie
Author:
Francois Marie Allioux
Author:
Zhenbang Cao
Author:
Joanna M. Biazik
Author:
Mohammad Tajik
Author:
Fei Deng
Author:
Yi Li
Author:
Roozbeh Abbasi
Author:
Mahroo Baharfar
Author:
Maedehsadat Mousavi
Author:
Dorna Esrafilzadeh
Author:
Kourosh Kalantar-Zadeh
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics