Cell-mediated biointerfacial phenolic assembly for probiotic nano encapsulation
Cell-mediated biointerfacial phenolic assembly for probiotic nano encapsulation
The use of cell-mediated chemistry is an emerging strategy that exploits the metabolic processes of living cells to develop biomimetic materials with advanced functionalities and enhanced biocompatibility. Here, a concept of a cell-mediated catalytic process for forming protective nano-shells on individual probiotic cells is demonstrated. This process is leveraged by the cell environment to induce oxidative polymerization of phenolic compounds, and simultaneously these phenolic polymers assemble to form nano-coatings around individual cell surfaces. The detailed analysis reveals that the oxidation process is triggered by an essential nutrient (manganese) of the probiotic cells, which significantly increases the oxidation rate of phenolic compounds. The phenolic coatings, encapsulating each cell in nanometre scale, demonstrate excellent biocompatibility and biodegradability. Additionally, the in situ encapsulated probiotic cells display an improved gastric tolerance of up to ≈1.4 times higher than the native cells and enhanced adhesion as high as ≈1.6 times onto a model of intestinal epithelial cells. Finally, the coated probiotic cells exhibit a high antioxidant activity as an advanced feature. Overall, this method provides a unique approach to improve the probiotic delivery using the cell machinery to engineer encapsulating nanocoatings with protective benefits and new functionalities.
biomimetic materials, nanocoatings, phenolic compounds, probiotic cells
Centurion, Franco
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Merhebi, Salma
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Baharfar, Mahroo
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Abbasi, Roozbeh
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Zhang, Chengchen
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Mousavi, Maedehsadat
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Xie, Wanjie
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Yang, Jiong
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Cao, Zhenbang
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Allioux, Francois Marie
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Harm, Gregory F.S.
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Biazik, Joanna
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Kalantar-Zadeh, Kourosh
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Rahim, Md Arifur
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24 June 2022
Centurion, Franco
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Merhebi, Salma
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Baharfar, Mahroo
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Abbasi, Roozbeh
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Zhang, Chengchen
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Mousavi, Maedehsadat
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Xie, Wanjie
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Yang, Jiong
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Cao, Zhenbang
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Allioux, Francois Marie
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Harm, Gregory F.S.
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Biazik, Joanna
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Kalantar-Zadeh, Kourosh
aded6a64-8612-40b7-aae9-233fbae916a6
Rahim, Md Arifur
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Centurion, Franco, Merhebi, Salma, Baharfar, Mahroo, Abbasi, Roozbeh, Zhang, Chengchen, Mousavi, Maedehsadat, Xie, Wanjie, Yang, Jiong, Cao, Zhenbang, Allioux, Francois Marie, Harm, Gregory F.S., Biazik, Joanna, Kalantar-Zadeh, Kourosh and Rahim, Md Arifur
(2022)
Cell-mediated biointerfacial phenolic assembly for probiotic nano encapsulation.
Advanced Functional Materials, 32 (26), [2200775].
(doi:10.1002/adfm.202200775).
Abstract
The use of cell-mediated chemistry is an emerging strategy that exploits the metabolic processes of living cells to develop biomimetic materials with advanced functionalities and enhanced biocompatibility. Here, a concept of a cell-mediated catalytic process for forming protective nano-shells on individual probiotic cells is demonstrated. This process is leveraged by the cell environment to induce oxidative polymerization of phenolic compounds, and simultaneously these phenolic polymers assemble to form nano-coatings around individual cell surfaces. The detailed analysis reveals that the oxidation process is triggered by an essential nutrient (manganese) of the probiotic cells, which significantly increases the oxidation rate of phenolic compounds. The phenolic coatings, encapsulating each cell in nanometre scale, demonstrate excellent biocompatibility and biodegradability. Additionally, the in situ encapsulated probiotic cells display an improved gastric tolerance of up to ≈1.4 times higher than the native cells and enhanced adhesion as high as ≈1.6 times onto a model of intestinal epithelial cells. Finally, the coated probiotic cells exhibit a high antioxidant activity as an advanced feature. Overall, this method provides a unique approach to improve the probiotic delivery using the cell machinery to engineer encapsulating nanocoatings with protective benefits and new functionalities.
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More information
e-pub ahead of print date: 22 March 2022
Published date: 24 June 2022
Additional Information:
Funding Information:
The authors thank Mr. Shaun Roux from Probiotics Australia Pty Ltd for the generous supply of probiotic cells, and Dr. Irene Moroni for the generous supply of the Caco-2 human colon adenocarcinoma cell line. In addition, the authors acknowledge the facilities, and the scientific and technical assistance of the Australian Microscopy & Microanalysis Research Facility at the Electron Microscope Unit, The University of New South Wales. The authors would like to acknowledge the NHMRC Development Grant No. APP1154969 for the financial coverage of this study. M.A.R. also acknowledges the ARC Discovery Early Career Researcher Award (DECRA) (Grant No. DE210101162). Open access publishing facilitated by University of New South Wales, as part of the Wiley - University of New South Wales agreement via the Council of Australian University Librarians.
Funding Information:
The authors thank Mr. Shaun Roux from Probiotics Australia Pty Ltd for the generous supply of probiotic cells, and Dr. Irene Moroni for the generous supply of the Caco‐2 human colon adenocarcinoma cell line. In addition, the authors acknowledge the facilities, and the scientific and technical assistance of the Australian Microscopy & Microanalysis Research Facility at the Electron Microscope Unit, The University of New South Wales. The authors would like to acknowledge the NHMRC Development Grant No. APP1154969 for the financial coverage of this study. M.A.R. also acknowledges the ARC Discovery Early Career Researcher Award (DECRA) (Grant No. DE210101162).
Publisher Copyright:
© 2022 The Authors. Advanced Functional Materials published by Wiley-VCH GmbH.
Keywords:
biomimetic materials, nanocoatings, phenolic compounds, probiotic cells
Identifiers
Local EPrints ID: 482310
URI: http://eprints.soton.ac.uk/id/eprint/482310
ISSN: 1616-301X
PURE UUID: 5657256e-a6c3-44ad-a847-cce0bd8b540f
Catalogue record
Date deposited: 26 Sep 2023 16:51
Last modified: 18 Mar 2024 04:15
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Contributors
Author:
Franco Centurion
Author:
Salma Merhebi
Author:
Mahroo Baharfar
Author:
Roozbeh Abbasi
Author:
Chengchen Zhang
Author:
Maedehsadat Mousavi
Author:
Wanjie Xie
Author:
Jiong Yang
Author:
Zhenbang Cao
Author:
Francois Marie Allioux
Author:
Gregory F.S. Harm
Author:
Joanna Biazik
Author:
Kourosh Kalantar-Zadeh
Author:
Md Arifur Rahim
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