Polyetherimide-grafted fe3O4@SiO2 nanoparticles as theranostic agents for simultaneous VEGF siRNA delivery and magnetic resonance cell imaging
Polyetherimide-grafted fe3O4@SiO2 nanoparticles as theranostic agents for simultaneous VEGF siRNA delivery and magnetic resonance cell imaging
Engineering a safe and high-efficiency delivery system for efficient RNA interference is critical for successful gene therapy. In this study, we designed a novel nanocarrier system of polyethyleneimine (PEI)-modified Fe3O4@SiO2, which allows high efficient loading of VEGF small hairpin (sh)RNA to form Fe3O4@SiO2/PEI/VEGF shRNA nanocomposites for VEGF gene silencing as well as magnetic resonance (MR) imaging. The size, morphology, particle stability, magnetic properties, and gene-binding capacity and protection were determined. Low cytotoxicity and hemolyticity against human red blood cells showed the excellent biocompatibility of the multifunctional nanocomposites, and also no significant coagulation was observed. The nanocomposites maintain their superparamagnetic property at room temperature and no appreciable change in magnetism, even after PEI modification. The qualitative and quantitative analysis of cellular internalization into MCF-7 human breast cancer cells by Prussian blue staining and inductively coupled plasma atomic emission spectroscopy analysis, respectively, demonstrated that the Fe3O4@SiO2/PEI/VEGF shRNA nanocomposites could be easily internalized by MCF-7 cells, and they exhibited significant inhibition of VEGF gene expression. Furthermore, the MR cellular images showed that the superparamagnetic iron oxide core of our Fe3O4@SiO2/PEI/VEGF shRNA nanocomposites could also act as a T2-weighted contrast agent for cancer MR imaging. Our data highlight multifunctional Fe3O4@SiO2/PEI/VEGF shRNA nanocomposites as a potential platform for simultaneous gene delivery and MR cell imaging, which are promising as theranostic agents for cancer treatment and diagnosis in the future.
Core/shell nanoparticle, MR imaging, PEI, Theranostics, VEGF silence
4279-4291
Li, Tingting
22947025-5c93-42ac-8d15-adc06a68e316
Shen, Xue
b334d366-7a1c-43a6-aebe-6e0e2d5be9f6
Chen, Yin
c7208435-64fb-42be-8c2a-922e6670d362
Zhang, Chengchen
abc47c06-4b99-4aed-be72-463f211e9dfa
Yan, Jie
6b311769-f4e4-44bc-ae1a-fcafc44d2491
Yang, Hong
728717f6-7f67-4e44-850c-272a39a20a07
Wu, Chunhui
72ab377b-a38d-47f0-9f5e-ddc02b4bb4b7
Zeng, Hongjun
a40d056b-c7c8-4df6-a495-62acee4b489c
Liu, Yiyao
f7cc88be-e992-4b2b-b4d1-9ca42e4aaf60
Li, Tingting
22947025-5c93-42ac-8d15-adc06a68e316
Shen, Xue
b334d366-7a1c-43a6-aebe-6e0e2d5be9f6
Chen, Yin
c7208435-64fb-42be-8c2a-922e6670d362
Zhang, Chengchen
abc47c06-4b99-4aed-be72-463f211e9dfa
Yan, Jie
6b311769-f4e4-44bc-ae1a-fcafc44d2491
Yang, Hong
728717f6-7f67-4e44-850c-272a39a20a07
Wu, Chunhui
72ab377b-a38d-47f0-9f5e-ddc02b4bb4b7
Zeng, Hongjun
a40d056b-c7c8-4df6-a495-62acee4b489c
Liu, Yiyao
f7cc88be-e992-4b2b-b4d1-9ca42e4aaf60
Li, Tingting, Shen, Xue, Chen, Yin, Zhang, Chengchen, Yan, Jie, Yang, Hong, Wu, Chunhui, Zeng, Hongjun and Liu, Yiyao
(2015)
Polyetherimide-grafted fe3O4@SiO2 nanoparticles as theranostic agents for simultaneous VEGF siRNA delivery and magnetic resonance cell imaging.
International Journal of Nanomedicine, 10, , [A313].
(doi:10.2147/IJN.S85095).
(In Press)
Abstract
Engineering a safe and high-efficiency delivery system for efficient RNA interference is critical for successful gene therapy. In this study, we designed a novel nanocarrier system of polyethyleneimine (PEI)-modified Fe3O4@SiO2, which allows high efficient loading of VEGF small hairpin (sh)RNA to form Fe3O4@SiO2/PEI/VEGF shRNA nanocomposites for VEGF gene silencing as well as magnetic resonance (MR) imaging. The size, morphology, particle stability, magnetic properties, and gene-binding capacity and protection were determined. Low cytotoxicity and hemolyticity against human red blood cells showed the excellent biocompatibility of the multifunctional nanocomposites, and also no significant coagulation was observed. The nanocomposites maintain their superparamagnetic property at room temperature and no appreciable change in magnetism, even after PEI modification. The qualitative and quantitative analysis of cellular internalization into MCF-7 human breast cancer cells by Prussian blue staining and inductively coupled plasma atomic emission spectroscopy analysis, respectively, demonstrated that the Fe3O4@SiO2/PEI/VEGF shRNA nanocomposites could be easily internalized by MCF-7 cells, and they exhibited significant inhibition of VEGF gene expression. Furthermore, the MR cellular images showed that the superparamagnetic iron oxide core of our Fe3O4@SiO2/PEI/VEGF shRNA nanocomposites could also act as a T2-weighted contrast agent for cancer MR imaging. Our data highlight multifunctional Fe3O4@SiO2/PEI/VEGF shRNA nanocomposites as a potential platform for simultaneous gene delivery and MR cell imaging, which are promising as theranostic agents for cancer treatment and diagnosis in the future.
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Accepted/In Press date: 13 May 2015
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Publisher Copyright:
© 2015 Li et al.
Keywords:
Core/shell nanoparticle, MR imaging, PEI, Theranostics, VEGF silence
Identifiers
Local EPrints ID: 482320
URI: http://eprints.soton.ac.uk/id/eprint/482320
ISSN: 1176-9114
PURE UUID: c8a2422a-ecf2-4b28-8e47-6123c75f1e85
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Date deposited: 26 Sep 2023 16:52
Last modified: 18 Mar 2024 04:15
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Contributors
Author:
Tingting Li
Author:
Xue Shen
Author:
Yin Chen
Author:
Chengchen Zhang
Author:
Jie Yan
Author:
Hong Yang
Author:
Chunhui Wu
Author:
Hongjun Zeng
Author:
Yiyao Liu
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