Bugs and drugs: a systems biology approach to characterising the effect of moxidectin on the horse’s faecal microbiome
Bugs and drugs: a systems biology approach to characterising the effect of moxidectin on the horse’s faecal microbiome
Background: Anthelmintic treatment is a risk factor for intestinal disease in the horse, known as colic. However the mechanisms involved in the onset of disease post anthelmintic treatment are unknown. The interaction between anthelmintic drugs and the gut microbiota may be associated with this observed increase in risk of colic. Little is known about the interaction between gut microbiota and anthelmintics and how treatment may alter microbiome function. The objectives of this study were: To characterise (1) faecal microbiota, (2) feed fermentation kinetics in vitro and (3) metabolic profiles following moxidectin administration to horses with very low (0 epg) adult strongyle burdens. Hypothesis: Moxidectin will not alter (1) faecal microbiota, (2) feed fermentation in vitro, or, (3) host metabolome. Results: Moxidectin increased the relative abundance of Deferribacter spp. and Spirochaetes spp. observed after 160 h in moxidectin treated horses. Reduced in vitro fibre fermentation was observed 16 h following moxidectin administration in vivo (P = 0.001), along with lower pH in the in vitro fermentations from the moxidectin treated group. Metabolic profiles from urine samples did not differ between the treatment groups. However metabolic profiles from in vitro fermentations differed between moxidectin and control groups 16 h after treatment (R 2 = 0.69, Q2Y = 0.48), and within the moxidectin group between 16 h and 160 h post moxidectin treatment (R 2 = 0.79, Q2Y = 0.77). Metabolic profiles from in vitro fermentations and fermentation kinetics both indicated altered carbohydrate metabolism following in vivo treatment with moxidectin. Conclusions: These data suggest that in horses with low parasite burdens moxidectin had a small but measurable effect on both the community structure and the function of the gut microbiome.
Anthelmintics, Cyathostomins, Horses, Metabolome, Microbiome, Microbiota, Multi-‘omics, Strongyles
Daniels, S. P.
1341913f-a1da-4748-bae1-1d470c91392f
Leng, J.
71755042-2b5f-44a6-8420-019f13a4a946
Swann, J. R.
7c11a66b-f4b8-4dbf-aa17-ad8b0561b85c
Proudman, C. J.
d8b66c8d-0e2d-40ae-81f7-6a960db240d4
December 2020
Daniels, S. P.
1341913f-a1da-4748-bae1-1d470c91392f
Leng, J.
71755042-2b5f-44a6-8420-019f13a4a946
Swann, J. R.
7c11a66b-f4b8-4dbf-aa17-ad8b0561b85c
Proudman, C. J.
d8b66c8d-0e2d-40ae-81f7-6a960db240d4
Daniels, S. P., Leng, J., Swann, J. R. and Proudman, C. J.
(2020)
Bugs and drugs: a systems biology approach to characterising the effect of moxidectin on the horse’s faecal microbiome.
Animal Microbiome, 2 (1), [38].
(doi:10.1186/s42523-020-00056-2).
Abstract
Background: Anthelmintic treatment is a risk factor for intestinal disease in the horse, known as colic. However the mechanisms involved in the onset of disease post anthelmintic treatment are unknown. The interaction between anthelmintic drugs and the gut microbiota may be associated with this observed increase in risk of colic. Little is known about the interaction between gut microbiota and anthelmintics and how treatment may alter microbiome function. The objectives of this study were: To characterise (1) faecal microbiota, (2) feed fermentation kinetics in vitro and (3) metabolic profiles following moxidectin administration to horses with very low (0 epg) adult strongyle burdens. Hypothesis: Moxidectin will not alter (1) faecal microbiota, (2) feed fermentation in vitro, or, (3) host metabolome. Results: Moxidectin increased the relative abundance of Deferribacter spp. and Spirochaetes spp. observed after 160 h in moxidectin treated horses. Reduced in vitro fibre fermentation was observed 16 h following moxidectin administration in vivo (P = 0.001), along with lower pH in the in vitro fermentations from the moxidectin treated group. Metabolic profiles from urine samples did not differ between the treatment groups. However metabolic profiles from in vitro fermentations differed between moxidectin and control groups 16 h after treatment (R 2 = 0.69, Q2Y = 0.48), and within the moxidectin group between 16 h and 160 h post moxidectin treatment (R 2 = 0.79, Q2Y = 0.77). Metabolic profiles from in vitro fermentations and fermentation kinetics both indicated altered carbohydrate metabolism following in vivo treatment with moxidectin. Conclusions: These data suggest that in horses with low parasite burdens moxidectin had a small but measurable effect on both the community structure and the function of the gut microbiome.
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Published date: December 2020
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Funding Information:
The authors would like to acknowledge Richard Ellis at the Animal and Plant Health Agency for conducting the sequencing. We would like to acknowledge Prof Meriel Moore-Colyer and technicians in the RAU laboratories for support with the fermentation experiment. We would also like to acknowledge Zoetis for suppling the moxidectin used in this study. Finally, we would like to acknowledge the owners of the horses used in this experiment.
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© 2020, The Author(s).
Keywords:
Anthelmintics, Cyathostomins, Horses, Metabolome, Microbiome, Microbiota, Multi-‘omics, Strongyles
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Local EPrints ID: 482434
URI: http://eprints.soton.ac.uk/id/eprint/482434
PURE UUID: 5e44a113-df34-48a4-a97f-e530ab733b16
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Date deposited: 03 Oct 2023 16:42
Last modified: 18 Mar 2024 03:56
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Author:
S. P. Daniels
Author:
J. Leng
Author:
C. J. Proudman
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