Identification of genes affecting glucose catabolism in nitrogen-limited fermentation
Identification of genes affecting glucose catabolism in nitrogen-limited fermentation
In recognition of the importance of assimilable nitrogen in the successful completion of several fermentation processes, we have sought to develop yeast strains that utilise this typically limited nutrient group more efficiently. With the aid of transposon mutagenesis together with a high-throughput method for analysis of multiple fermentations, we have identified 'nitrogen-efficient' mutants that catabolise more sugar for a given amount of nitrogen utilised. In this way we have identified two genes, NGR1 and GID7, whose disruption leads to an enhanced catabolism of sugar in an industrial strain and/or a laboratory strain, during growth in a chemically defined grape juice medium with limiting nitrogen. Deletion of NGR1 or GID7 also resulted in minor changes in metabolites produced, and biomass yield, measured as dry weight, was also decreased in NGR1 mutant strains.
Assimilable nitrogen, Fermentation, Industrial biotechnology, Transposon mutagenesis, Wine, Yeast
791-800
Gardner, Jennifer M.
0d95188b-206d-4817-8437-e163351f6e7f
McBryde, Colin
bceddd45-eb75-408e-9e11-3a0377e54899
Vystavelova, Andrea
44f98fd1-da41-4e51-82ff-843613ad8249
De Barros Lopes, Miguel
29cf7f9c-cfd5-451c-a849-75cf000b510b
Jiranek, Vladimir
8e5a8dfd-f5b2-43e3-928b-11dff324abc7
June 2005
Gardner, Jennifer M.
0d95188b-206d-4817-8437-e163351f6e7f
McBryde, Colin
bceddd45-eb75-408e-9e11-3a0377e54899
Vystavelova, Andrea
44f98fd1-da41-4e51-82ff-843613ad8249
De Barros Lopes, Miguel
29cf7f9c-cfd5-451c-a849-75cf000b510b
Jiranek, Vladimir
8e5a8dfd-f5b2-43e3-928b-11dff324abc7
Gardner, Jennifer M., McBryde, Colin, Vystavelova, Andrea, De Barros Lopes, Miguel and Jiranek, Vladimir
(2005)
Identification of genes affecting glucose catabolism in nitrogen-limited fermentation.
FEMS Yeast Research, 5 (9), .
(doi:10.1016/j.femsyr.2005.02.008).
Abstract
In recognition of the importance of assimilable nitrogen in the successful completion of several fermentation processes, we have sought to develop yeast strains that utilise this typically limited nutrient group more efficiently. With the aid of transposon mutagenesis together with a high-throughput method for analysis of multiple fermentations, we have identified 'nitrogen-efficient' mutants that catabolise more sugar for a given amount of nitrogen utilised. In this way we have identified two genes, NGR1 and GID7, whose disruption leads to an enhanced catabolism of sugar in an industrial strain and/or a laboratory strain, during growth in a chemically defined grape juice medium with limiting nitrogen. Deletion of NGR1 or GID7 also resulted in minor changes in metabolites produced, and biomass yield, measured as dry weight, was also decreased in NGR1 mutant strains.
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Published date: June 2005
Additional Information:
Funding Information:
This project is supported by Australia’s grapegrowers and winemakers through their investment body the Grape and Wine Research and Development Corporation (GWRDC), with matching funds from the Australian Government (projects UA 00/5 and UA 01/04). Further support was kindly provided by Lallemand Australia. We wish to thank Kate Poole for strains KP2 and KP3, Michael Snyder for a gift of the transposon insertion library, Will Gardner for manufacture of the 48-pinned replicator, and Sakkie Pretorius and Michelle Walker for critical reading of the manuscript. Jennifer Gardner is a holder of an Australian Postgraduate Award. Colin McBryde is a holder of a Postgraduate Award from The University of Adelaide as well as a supplementary scholarship from the GWRDC.
Keywords:
Assimilable nitrogen, Fermentation, Industrial biotechnology, Transposon mutagenesis, Wine, Yeast
Identifiers
Local EPrints ID: 482557
URI: http://eprints.soton.ac.uk/id/eprint/482557
ISSN: 1567-1356
PURE UUID: b77012a2-17b9-47b4-ad9f-bb7b55c5eac3
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Date deposited: 10 Oct 2023 16:58
Last modified: 18 Mar 2024 04:12
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Contributors
Author:
Jennifer M. Gardner
Author:
Colin McBryde
Author:
Andrea Vystavelova
Author:
Miguel De Barros Lopes
Author:
Vladimir Jiranek
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