The evolution of Lachancea thermotolerans is driven by geographical determination, anthropisation and flux between different ecosystems
The evolution of Lachancea thermotolerans is driven by geographical determination, anthropisation and flux between different ecosystems
The yeast Lachancea thermotolerans (formerly Kluyveromyces thermotolerans) is a species with remarkable, yet underexplored, biotechnological potential. This ubiquist occupies a range of natural and anthropic habitats covering a wide geographic span. To gain an insight into L. thermotolerans population diversity and structure, 172 isolates sourced from diverse habitats worldwide were analysed using a set of 14 microsatellite markers. The resultant clustering revealed that the evolution of L. thermotolerans has been driven by the geography and ecological niche of the isolation sources. Isolates originating from anthropic environments, in particular grapes and wine, were genetically close, thus suggesting domestication events within the species. The observed clustering was further validated by several means including, population structure analysis, F-statistics, Mantel's test and the analysis of molecular variance (AMOVA). Phenotypic performance of isolates was tested using several growth substrates and physicochemical conditions, providing added support for the clustering. Altogether, this study sheds light on the genotypic and phenotypic diversity of L. thermotolerans, contributing to a better understanding of the population structure, ecology and evolution of this non-Saccharomyces yeast.
Hranilovic, Ana
24ae38eb-be9f-4d06-b7c5-e0f9cb9c9c21
Bely, Marina
244fa32d-0980-4903-8e38-92464025359f
Masneuf-Pomarede, Isabelle
71059697-e30e-44a5-b841-35388db19473
Jiranek, Vladimir
8e5a8dfd-f5b2-43e3-928b-11dff324abc7
Albertin, Warren
58c80d3f-18ec-41fa-8cd1-098c105adedb
September 2017
Hranilovic, Ana
24ae38eb-be9f-4d06-b7c5-e0f9cb9c9c21
Bely, Marina
244fa32d-0980-4903-8e38-92464025359f
Masneuf-Pomarede, Isabelle
71059697-e30e-44a5-b841-35388db19473
Jiranek, Vladimir
8e5a8dfd-f5b2-43e3-928b-11dff324abc7
Albertin, Warren
58c80d3f-18ec-41fa-8cd1-098c105adedb
Hranilovic, Ana, Bely, Marina, Masneuf-Pomarede, Isabelle, Jiranek, Vladimir and Albertin, Warren
(2017)
The evolution of Lachancea thermotolerans is driven by geographical determination, anthropisation and flux between different ecosystems.
PLoS ONE, 12 (9), [e0184652].
(doi:10.1371/journal.pone.0184652).
Abstract
The yeast Lachancea thermotolerans (formerly Kluyveromyces thermotolerans) is a species with remarkable, yet underexplored, biotechnological potential. This ubiquist occupies a range of natural and anthropic habitats covering a wide geographic span. To gain an insight into L. thermotolerans population diversity and structure, 172 isolates sourced from diverse habitats worldwide were analysed using a set of 14 microsatellite markers. The resultant clustering revealed that the evolution of L. thermotolerans has been driven by the geography and ecological niche of the isolation sources. Isolates originating from anthropic environments, in particular grapes and wine, were genetically close, thus suggesting domestication events within the species. The observed clustering was further validated by several means including, population structure analysis, F-statistics, Mantel's test and the analysis of molecular variance (AMOVA). Phenotypic performance of isolates was tested using several growth substrates and physicochemical conditions, providing added support for the clustering. Altogether, this study sheds light on the genotypic and phenotypic diversity of L. thermotolerans, contributing to a better understanding of the population structure, ecology and evolution of this non-Saccharomyces yeast.
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Published date: September 2017
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Funding Information:
Funding: This research was conducted by the Australian Research Council Industrial Transformation Training Centre for Innovative Wine Production (Project IC130100005) to VJ with support from the Wine Australia and industry partners. AH received an ISVV Travel Grant and Wine Australia Travel Grant (AGT1524). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
Copyright © 2017 Hranilovic et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Local EPrints ID: 482626
URI: http://eprints.soton.ac.uk/id/eprint/482626
ISSN: 1932-6203
PURE UUID: 11782fb4-77cf-475f-9aa3-25520971a790
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Date deposited: 10 Oct 2023 17:01
Last modified: 18 Mar 2024 04:12
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Author:
Ana Hranilovic
Author:
Marina Bely
Author:
Isabelle Masneuf-Pomarede
Author:
Vladimir Jiranek
Author:
Warren Albertin
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