The VvBAP1 gene is identified as a potential inhibitor of cell death in grape berries
The VvBAP1 gene is identified as a potential inhibitor of cell death in grape berries
Cell death (CD) in Vitis vinifera L grape berries, exemplified in Shiraz, occurs late in ripening influencing yield, berry and wine quality. Here we isolated and functionally characterised a BON1-associated gene, VvBAP1 from Shiraz berries, encoding a small protein with a C2 domain. VvBAP1 transcript increased during fruit development from veraison to harvest, and was significantly inhibited by drought stress 92 days after flowering when CD normally begins. This was correlated with high CD in Shiraz berries. The agrobacterium-mediated transient expression of VvBAP1 in tobacco leaves led to a decrease in electrolyte leakage and downregulated a marker gene (Hsr203J) for cell death. Expressing VvBAP1 in yeast (Saccharomyces cerevisiae) also alleviated cell death induced by hydrogen peroxide (H2O2). Overexpression of VvBAP1 in Arabidopsis increased resistance to H2O2 and reduced CD due to higher expression of genes involved in anti-oxidative responses. Arabidopsis overexpressing VvBAP1 displayed higher tolerance to drought accompanied by upregulation of antioxidant-related gene expression. VvBAP1 complemented an Arabidopsis bap1 knockout by abolishing its CD phenotypes. These results indicate that VvBAP1 may play a role in alleviating CD in grape berries and its downregulation under drought stress may be responsible for the generally observed increase in CD within the berry.
BAP1, drought stress, grape berry, HO, reactive oxygen species, ROS, Syrah
428-442
Cao, Shifeng
895e7434-ade7-4ee2-9048-aa61d9273da5
Xiao, Zeyu
e063dcb6-bbb2-4504-84cb-37774c636973
Jiranek, Vladimir
8e5a8dfd-f5b2-43e3-928b-11dff324abc7
Tyerman, Stephen D.
3ac7a8f5-82c9-434e-a866-1c6abae7e402
2019
Cao, Shifeng
895e7434-ade7-4ee2-9048-aa61d9273da5
Xiao, Zeyu
e063dcb6-bbb2-4504-84cb-37774c636973
Jiranek, Vladimir
8e5a8dfd-f5b2-43e3-928b-11dff324abc7
Tyerman, Stephen D.
3ac7a8f5-82c9-434e-a866-1c6abae7e402
Cao, Shifeng, Xiao, Zeyu, Jiranek, Vladimir and Tyerman, Stephen D.
(2019)
The VvBAP1 gene is identified as a potential inhibitor of cell death in grape berries.
Functional Plant Biology, 46 (5), .
(doi:10.1071/FP18272).
Abstract
Cell death (CD) in Vitis vinifera L grape berries, exemplified in Shiraz, occurs late in ripening influencing yield, berry and wine quality. Here we isolated and functionally characterised a BON1-associated gene, VvBAP1 from Shiraz berries, encoding a small protein with a C2 domain. VvBAP1 transcript increased during fruit development from veraison to harvest, and was significantly inhibited by drought stress 92 days after flowering when CD normally begins. This was correlated with high CD in Shiraz berries. The agrobacterium-mediated transient expression of VvBAP1 in tobacco leaves led to a decrease in electrolyte leakage and downregulated a marker gene (Hsr203J) for cell death. Expressing VvBAP1 in yeast (Saccharomyces cerevisiae) also alleviated cell death induced by hydrogen peroxide (H2O2). Overexpression of VvBAP1 in Arabidopsis increased resistance to H2O2 and reduced CD due to higher expression of genes involved in anti-oxidative responses. Arabidopsis overexpressing VvBAP1 displayed higher tolerance to drought accompanied by upregulation of antioxidant-related gene expression. VvBAP1 complemented an Arabidopsis bap1 knockout by abolishing its CD phenotypes. These results indicate that VvBAP1 may play a role in alleviating CD in grape berries and its downregulation under drought stress may be responsible for the generally observed increase in CD within the berry.
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Published date: 2019
Additional Information:
Funding Information:
WethanktheArabidopsisBioresourceCenterforprovidingT-DNAinsertion lines, Professor Dabing Zhang (The University of Adelaide) for plasmids and the Agrobacterium tumefaciens strain GV3101, Dr Stefanie Wege for transient expression on tobacco, Dr Johannes Scharwies for drought stress treatment on Arabidopsis and Dr Xiujuan Yang for yeast two hybrid and BiFC. We thank Dr Victor Sadras and the South Australian Research and Development Institute for access to the drought treated Shiraz vines for berry samples. This research was conducted by the Australian Research Council Training Centre for Innovative Wine Production (www.adelaide. edu.au/tc-iwp/), which is funded as a part of the ARC’s Industrial Transformation Research Program (Project No IC130100005) with support from Wine Australia and industry partners.
Publisher Copyright:
© 2019 CSIRO.
Keywords:
BAP1, drought stress, grape berry, HO, reactive oxygen species, ROS, Syrah
Identifiers
Local EPrints ID: 482679
URI: http://eprints.soton.ac.uk/id/eprint/482679
ISSN: 1445-4408
PURE UUID: c061749a-2868-46fa-8cc5-4783c43d731b
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Date deposited: 11 Oct 2023 16:51
Last modified: 18 Mar 2024 04:12
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Contributors
Author:
Shifeng Cao
Author:
Zeyu Xiao
Author:
Vladimir Jiranek
Author:
Stephen D. Tyerman
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