READ ME File For 'Dataset for the thesis "Developing SERS-based point-of-care inflammatory monitoring for risk identification of worsening progression of age-related disease"' Dataset DOI: 10.5258/SOTON/D2222 ReadMe Author: Rachel Kidd, University of Southampton This dataset supports the thesis entitled "Developing SERS-based point-of-care inflammatory monitoring for risk identification of worsening progression of age-related disease"' AWARDED BY: Univeristy of Southampton DATE OF AWARD: 2022 Date of data collection: March 2016 - August 2019 Date that the file was originally created: 17th May 2022 Information about geographic location of data collection: Data collected at the University of Southampton License: CC BY NC SA ------------------- DATA & FILE OVERVIEW -------------------- This dataset contains: CH2 Hearing loss and Inflammation Data.xlsx All the data that was collected to contribute to the results shown in chapter 2 of the thesis. (This chapter data is available 'on request' to bone fide researchers with ethical clearance. Please complete the attached request form and return it to researchdata@soton.ac.uk) CH3 Developing SERS Sensors Data.xlsx All the data that was collected to contribute to the results shown in chapter 3 of the thesis. CH3 SERS Raw Data Files (zip) The raw SERS data files after pre-processing for neopterin standards, creatinine standards, real urine neopterin measurement, real urine creatinine measurement, artificial urine neopterin measurement and artificial urine creatinine measurement. CH3 HPLC Artificial urine raw data.xlsx CH3 HPLC Real Urine Raw Data.xlsx CH4 Developing lateral flow device data.xlsx All the data that was collected to contribute to the results shown in chapter 4 of the thesis. -------------------------- METHODOLOGICAL INFORMATION -------------------------- Description of methods used for collection/generation of data: All methods used for collection/generation of data can be found in the thesis. Methods for processing the data: Methods for processing data can be found in the thesis. -------------------------- DATA-SPECIFIC INFORMATION -------------------------- CH2 Hearing loss and inflammation data Mass Spec UNCR Number of variables: 18 Number of cases/rows: 61 Variable list, defining any abbreviations, units of measure, codes or symbols used: ID - participant ID number Month 1 Month 2 Month 3 Month 4 Month 5 Month 6 Month 7 Month 8 Month 9 Month 10 Month 11 Month 12 Average - average value over the 12 months Standard deviation- SD of the average AUC/number of samples - area under curve divided by number of samples No. of samples - number of samples measured (out of 12) No. of times above 251- number of times the value months 1-12) was above 251 Baseline Hearing Number of variables:32 Number of cases/rows: 61 Variable list, defining any abbreviations, units of measure, codes or symbols used: ID - Participant ID number 1R250 - Baseline right ear 250 Hz 1R500 Baseline right ear 500 Hz 1R1000 Baseline right ear 1000 Hz 1R2000 1R3000 1R4000 1R6000 1R8000 1R12000 1L250 Baseline left ear 250 Hz 1L500 Baseline left ear 500 Hz 1L1000 1L2000 1L3000 1L4000 1L6000 1L8000 1L12000 PTA_AV(250-8000) Baseline PTA average calculated 250-8000 Hz RPTA_AV(250-8000) Baseling right ear PTA Average LPTA_AV(250-8000) Baseline left ear PTA average LFA(250-1000) Baseline low frequency average calculated 250-1000Hz RLFA(250-1000) Baseline right low freq average LLFA(250-1000) Baseline left low freq average HFA(4000-8000) Baseline high frequency average (calculated 4000-8000Hz) RHFA(4000-8000) Baseline right high freq average LHFA(4000-8000) Baseline left high freq average WE_PTA_Av Baseline worse ear pure tone average WE_LFA Baseline worse ear low frequency average WE_HFA Baseline worse ear high frequency average Baseline degree of loss 0 = normal, 1= mild 2= moderate 3= severe Year 2 Hearing Number of variables:32 Number of cases/rows: 61 Variable list, defining any abbreviations, units of measure, codes or symbols used: as above Year 3 Hearing Number of variables:32 Number of cases/rows: 61 Variable list, defining any abbreviations, units of measure, codes or symbols used: as above Questionnaire Number of variables: 61 Number of cases/rows: 61 Variable list, defining any abbreviations, units of measure, codes or symbols used: ID Participant ID number AGE at start age at start of study Age at 3rd test age at end of study GENDER gender 1=male 2=female OCCUPATION HEARINGQUIET difficulty hearing in quiet HEARINGNOISE difficulty hearing in noise NOISEXP history of noise exposure EXP DURATION duration of noise exposure TINNITUS tinnitus FHX family history of hearing loss PHX previous history of hearing problems HA hearing aid user SMOKING history of smoking GIVEUP given up before the age of 40 HYPERTENSION diagnosed with hypertension STATIN USE prescribed statins ANGINA/VALVULAR Dx diagnosis of angina or valvular disease STROKE history of stroke DM diagnosis of diabetes mellitus CVS Dx diagnosis of cardiovascular disease 1 or more Dx (CV or DM) 1 or more diagnoses of cardiovascular disease or diabetes 1_WBC(X109/L) Basline white blood cell count 1_NEU(X109/L) Baseline neutrophil count 1-LYMP(X109/L) Baseline lymphocyte count 1-MONO(X109/L) Baseline monocyte count 1-EOSIN(X109/L) Baseline eosinophil count 1-BASO(X109/L) Baseline basophil count 1-%NEU Baseline neutrophil % 1-%LYM Baseline lymphocyte % 1_%MON Baseline monocyte % 1_%EOS Baseline eosoniphil % 1_%BAS Baseine basophil % 2_WBC(X109/L) year 2 white blood cell count 2_NEU(X109/L) year 2 neutrophil count 2_LYMP(X109/L) 2_MONO(X109/L) 2_EOSIN(X109/L) 2_BASO(X109/L) 2_%NEU 2_%LYM 2_%MON 2_%EOS 2_%BAS Av_WBC average white blood cell count HEARINGQUIET study end difficulty hearing in quiet HEARINGNOISE study end difficulty hearing in noise NOISEXP EXP DURATION TINNITUS FHX PHX HA SMOKING GIVEUP HYPERTENSION STATIN USE ANGINA/VALVULAR Dx STROKE DM CVS Dx Year 3 AUD Averages Number of variables: 37 Number of cases/rows: 61 Variable list, defining any abbreviations, units of measure, codes or symbols used: ID Participant ID number 3PTA_AV(250-8000) study end pure tone average 3RPTA_AV(250-8000) study end right ear pure tone average 3LPTA_AV(250-8000) study end left ear pure tone average 3LFA(250-1000) study end low frequency average 3RLFA(250-1000) study end right ear low freq average 3LLFA(250-1000) study end left ear low freq average 3HFA(4000-8000) study end high freq average 3RHFA(4000-8000) study end right ear high freq average 3LHFA(4000-8000) study end left ear high freq average 3WE_PTA_Av study end worse ear pure tone average 3WE_LFA study end worse ear low freq average 3WE_HFA study end worse ear high freq average PTA_AV(250-8000) Baseline pure tone average RPTA_AV(250-8000) Baseline right ear pure tone average LPTA_AV(250-8000) Baseline left ear pure tone average LFA(250-1000) Baseline low freq average RLFA(250-1000) Baseline right ear low freq average LLFA(250-1000) Baseline left ear low freq average HFA(4000-8000) Baseline high freq average RHFA(4000-8000) Baseline right ear high freq average LHFA(4000-8000) Baseline left ear high freq average WE_PTA_Av Baseline worst ear pure tone average WE_LFA Baseline worse ear low freq average WE_HFA Baseline worse ear high freq average DIFF_PTA_AV(250-8000) Difference between baseline and study end pure tone average DIFF_RPTA_AV(250-8000) Difference between baseline and study end right ear pure tone average DIFF_LPTA_AV(250-8000) Difference between baseline and study end left ear pure tone average DIFF_LFA(250-1000) Difference between baseline and study end low freq average DIFF_RLFA(250-1000) Difference between baseline and study end right ear low freq average DIFF_LLFA(250-1000) Difference between baseline and study end left ear low freq av DIFF_HFA(4000-8000) Difference between baseline and study end high freq av DIFF_RHFA(4000-8000) Difference between baseline and study end right ear high freq av DIFF_LHFA(4000-8000) Difference between baseline and study end left ear high freq av DIFF_WE_PTA_Av Difference between baseline and study end worst ear PTA Av DIFF_WE_LFA Difference between baseline and study end worse ear LFA DIFF_WE_HFA Difference between baseline and study end worse ear HFA TEOAE Number of variables:37 Number of cases/rows: 61 Variable list, defining any abbreviations, units of measure, codes or symbols used: ID Year 1 Reproducibility R- Year one Transient OAE right ear reproducibility Year 1 Reproducibility L- Year one Transient OAE left ear reproducibility 1_R_SNR1k Year 1 Transient OAE right ear 1000Hz 1_R_SNR1.5k Year 1 " " " 1500Hz 1_R_SNR2k Year 1 " " " 2000 Hz 1_R_SNR3k 1_R_SNR4k 1_L_SNR1k Year 1 Transient OAE left ear 1000 Hz 1_L_SN1.5k 1_L_SNR2k 1_L_SNR3k 1_L_SNR4k Year 1 Transient OAE left ear 4000 Hz Year 2 Reproducibility R- Year 2 Transient OAE right ear reproducibility Year 2 Reproducibility L- Year 2 Transient OAE left ear reproducibility 2_R_SNR1k Year 2 Transient OAE right ear 1000 Hz 2_R_SNR1.5k Year 2 Transient OAE right ear 1500Hz 2_R_SNR2k 2_R_SNR3k 2_R_SNR4k 2_L_SNR1k Year 2 Transient OAE left ear 1000Hz 2_L_SNR1.5k 2_L_SNR2k 2_L_SNR3k 2_L_SNR4k Year 2 Transient OAE left ear 4000Hz Year 3 Reproducibility R- Year 3 Transient OAE right ear reproducibility Year 3 Reproducibility L- Year 3 Transient OAE left ear reproducibility 3_R_SNR1k Year 3 Transient OAE right ear 1000 Hz 3_R_SNR1.5k 3_R_SNR2k 3_R_SNR3k 3_R_SNR4k 3_L_SNR1k Year 3 Transient OAE left ear 1000 Hz 3_L_SNR1.5k 3_L_SNR2k 3_L_SNR3k 3_L_SNR4k CH3 Developing SERS Sensors Data SERS Neopterin Standards Number of variables:4 Number of cases/rows: 9 Variable list, defining any abbreviations, units of measure, codes or symbols used: Concentration (micromolar) X Normalised peak area mean Normalised peak area CV% Normalised peak area N number SERS Creatinine standards Number of variables:4 Number of cases/rows: 9 Variable list, defining any abbreviations, units of measure, codes or symbols used: Concentration (milliomolar) X Normalised peak area mean Y Normalised peak area CV% Normalised peak area N number HPLC Creatinine standards Number of variables: 4 Number of cases/rows: 10 Variable list, defining any abbreviations, units of measure, codes or symbols used: Concentration (millimolar) X Background subtracted spectrum 1 Y Background subtracted spectrum 2 Y Background subtracted spectrum 3 Y HPLC Neopterin Standards Number of variables:3 Number of cases/rows: 11 Variable list, defining any abbreviations, units of measure, codes or symbols used: Concentration (micromolar) X Area under peak 1 Y Area under peak 2Y Neopterin standard addition raw Number of variables: 6 Number of cases/rows: 1975 Variable list, defining any abbreviations, units of measure, codes or symbols used: Wavenumber X 0 microliter of neopterin standard added 5 microliters of neopterin standard added 10 microliters of neopterin standard added 20 microliters of neopterin standard added 40 microliters of neopterin standard added Neopterin Standard addition peaks Number of variables: 5 Number of cases/rows: 5 Variable list, defining any abbreviations, units of measure, codes or symbols used: Quantity of standard added Peak height Peak area Curve fitted peak height Curve fitted peak area CH3 Real Urine Number of variables:10 Number of cases/rows: 58 Variable list, defining any abbreviations, units of measure, codes or symbols used: Sample ID Creatinine conc 1 Creatinine conc 2 Creatinine conc 3 Neopterin conc 1 Neopterin conc 2 Neopterin conc 3 Urine neopterin to creatinine ratio 1 Urine neopterin to creatinine ratio 2 Urine neipterin to creatinine ratio 3 CH3 Artificial Urine Number of variables: 13 Number of cases/rows: 14 Variable list, defining any abbreviations, units of measure, codes or symbols used: Sample ID Expected Creatinine concentration Measured creatinine concentration 1 Measured creatinine concentration 2 Measured creatinine concentration 3 Expected Neopterin concentration Measured Neopterin concentration 1 Measured Neopterin concentration 2 Measured Neopterin concentration 3 Expected urine neopterin to creatinine ratio Measured urine neopterin to creatinine ratio 1 Measured urine neopterin to creatinine ratio 2 Measured urine neopterin to creatinine ratio 3 Batch to batch AUC Number of variables:4 Number of cases/rows: 100+ Variable list, defining any abbreviations, units of measure, codes or symbols used: Area under the curve of measured peak for 100 measurements of one month old batch of SERS sensors, batch a SERS sensors, batch b SERS sensors and Au nanoparticle collide Fig 3.3. UV Vis SERS Sensors Number of variables: 3 Number of cases/rows: 1000 Variable list, defining any abbreviations, units of measure, codes or symbols used: Wavelength/nm X Average Intensity/AU Y Normalised Intensity/AU Y Fig 3.3 UV Vis 40nm AuNPs Number of variables: 3 Number of cases/rows: 1000 Variable list, defining any abbreviations, units of measure, codes or symbols used: Wavelength/nm X Average Intensity/AU Y Normalised Intensity/AU Y Fig 3.3 Soak Time Number of variables:2 Number of cases/rows: 19 Variable list, defining any abbreviations, units of measure, codes or symbols used: Time/mins X Area under curve Y Fig 3.3 Acquisition Time Number of variables: 2 Number of cases/rows: 6 Variable list, defining any abbreviations, units of measure, codes or symbols used: Time/mins X Area under curve Y Fig 3.3 Laser Power degradation Number of variables:4 Number of cases/rows: 30 Variable list, defining any abbreviations, units of measure, codes or symbols used: Number of accumulations 1-30 Area under curve with 100% laser power Area under curve with 50% laser power Area under curve with 25% laser power CH4 Developing Lateral Flow Device Data CH4 Fig 4.5 Number of variables: 7 Number of cases/rows: 1074 Variable list, defining any abbreviations, units of measure, codes or symbols used: CH4 Fig 4.6 Number of variables:10 Number of cases/rows: 9 Variable list, defining any abbreviations, units of measure, codes or symbols used: Concentration (micromolar) Peak height at 745 cm-1 1 Peak height at 745 cm-1 2 Peak height at 745 cm-1 3 Peak height at 680 cm-1 1 Peak height at 680 cm-1 2 Peak height at 680 cm-1 3 Peak height ratio 1 Peak height ratio 2 Peak height ratio 3 CH4 Fig 4.7D Number of variables:8 Number of cases/rows: 1074 Variable list, defining any abbreviations, units of measure, codes or symbols used: Wavenumber X SERS Intensity for 5.5 cm run device 1 SERS Intensity for 5.5 cm run device 2 SERS Intensity for 6.0 cm run device 1 SERS Intensity for 6.0 cm run device 2 SERS Intensity for 6.5cm run device 1 SERS Intensity for 6.5cm run device 2 SERS Intensity for 7.0cm run device 1 CH4 Neopterin on G1 Device Number of variables: 7 Number of cases/rows: 9 Variable list, defining any abbreviations, units of measure, codes or symbols used: Concentration (micromolar) Peak Ratio Height Mean Peak Ratio Height Standard deviation Peak Ratio Height n number Peak ratio area Mean Peak ratio area standard deviation Peak ratio area n number CH4 Neopterin on WN1 Devices Number of variables: 7 Number of cases/rows: 9 Variable list, defining any abbreviations, units of measure, codes or symbols used: Concentration (micromolar) Peak Ratio Height Mean Peak Ratio Height Standard deviation Peak Ratio Height n number Peak ratio area Mean Peak ratio area standard deviation Peak ratio area n number Date that the file was created: December 2022