QTL mapping: an innovative method for investigating the genetic determinism of yeast-bacteria interactions in wine
QTL mapping: an innovative method for investigating the genetic determinism of yeast-bacteria interactions in wine
Abstract: The two most commonly used wine microorganisms, Saccharomyces cerevisiae yeast and Oenococcus oeni bacteria, are responsible for completion of alcoholic and malolactic fermentation (MLF), respectively. For successful co-inoculation, S. cerevisiae and O. oeni must be able to complete fermentation; however, this relies on compatibility between yeast and bacterial strains. For the first time, quantitative trait loci (QTL) analysis was used to elucidate whether S. cerevisiae genetic makeup can play a role in the ability of O. oeni to complete MLF. Assessment of 67 progeny from a hybrid S. cerevisiae strain (SBxGN), co-inoculated with a single O. oeni strain, SB3, revealed a major QTL linked to MLF completion by O. oeni. This QTL encompassed a well-known translocation, XV-t-XVI, that results in increased SSU1 expression and is functionally linked with numerous phenotypes including lag phase duration and sulphite export and production. A reciprocal hemizygosity assay was performed to elucidate the effect of the gene SSU1 in the SBxGN background. Our results revealed a strong effect of SSU1 haploinsufficiency on O. oeni’s ability to complete malolactic fermentation during co-inoculation and pave the way for the implementation of QTL mapping projects for deciphering the genetic bases of microbial interactions. Key points: • For the first time, QTL analysis has been used to study yeast-bacteria interactions. • A QTL encompassing a translocation, XV-t-XVI, was linked to MLF outcomes. • S. cerevisiae SSU1 haploinsufficiency positively impacted MLF by O. oeni.
Malolactic fermentation, QTL, SSU1, Wine, Yeast-bacteria interactions
5053-5066
Bartle, Louise
fb99e607-0839-493f-993b-cde6aa61beb3
Peltier, Emilien
b92254ff-60b3-40ac-aa49-26a864a694d2
Sundstrom, Joanna F.
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Sumby, Krista
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Mitchell, James G.
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Jiranek, Vladimir
8e5a8dfd-f5b2-43e3-928b-11dff324abc7
Marullo, Philippe
e81e3634-253f-488e-bba7-b44b3a82dae6
June 2021
Bartle, Louise
fb99e607-0839-493f-993b-cde6aa61beb3
Peltier, Emilien
b92254ff-60b3-40ac-aa49-26a864a694d2
Sundstrom, Joanna F.
6c6b3452-dfb3-4b5c-aa42-c721eed7b9bb
Sumby, Krista
f54b8f1c-c29e-488b-ab5e-dbb9b2576ba9
Mitchell, James G.
ca19546a-f575-428c-88aa-1b2e3d7d6de8
Jiranek, Vladimir
8e5a8dfd-f5b2-43e3-928b-11dff324abc7
Marullo, Philippe
e81e3634-253f-488e-bba7-b44b3a82dae6
Bartle, Louise, Peltier, Emilien, Sundstrom, Joanna F., Sumby, Krista, Mitchell, James G., Jiranek, Vladimir and Marullo, Philippe
(2021)
QTL mapping: an innovative method for investigating the genetic determinism of yeast-bacteria interactions in wine.
Applied Microbiology and Biotechnology, 105 (12), .
(doi:10.1007/s00253-021-11376-x).
Abstract
Abstract: The two most commonly used wine microorganisms, Saccharomyces cerevisiae yeast and Oenococcus oeni bacteria, are responsible for completion of alcoholic and malolactic fermentation (MLF), respectively. For successful co-inoculation, S. cerevisiae and O. oeni must be able to complete fermentation; however, this relies on compatibility between yeast and bacterial strains. For the first time, quantitative trait loci (QTL) analysis was used to elucidate whether S. cerevisiae genetic makeup can play a role in the ability of O. oeni to complete MLF. Assessment of 67 progeny from a hybrid S. cerevisiae strain (SBxGN), co-inoculated with a single O. oeni strain, SB3, revealed a major QTL linked to MLF completion by O. oeni. This QTL encompassed a well-known translocation, XV-t-XVI, that results in increased SSU1 expression and is functionally linked with numerous phenotypes including lag phase duration and sulphite export and production. A reciprocal hemizygosity assay was performed to elucidate the effect of the gene SSU1 in the SBxGN background. Our results revealed a strong effect of SSU1 haploinsufficiency on O. oeni’s ability to complete malolactic fermentation during co-inoculation and pave the way for the implementation of QTL mapping projects for deciphering the genetic bases of microbial interactions. Key points: • For the first time, QTL analysis has been used to study yeast-bacteria interactions. • A QTL encompassing a translocation, XV-t-XVI, was linked to MLF outcomes. • S. cerevisiae SSU1 haploinsufficiency positively impacted MLF by O. oeni.
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Published date: June 2021
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Funding Information:
This work was supported by Australia’s grape growers and winemakers through their investment body, Wine Australia, with matching funds from the Australian Government. LB was supported by joint scholarships from The University of Adelaide and Wine Australia (AGW Ph 1510). JS was supported by Wine Australia project funding (UA1707). JS, KS and VJ are supported by The Australian Research Council Training Centre for Innovative Wine Production ( www.ARCwinecentre.org.au ; project number IC170100008), which is funded by the Australian Government with additional support from Wine Australia and industry partners. The University of Adelaide is a member of the Wine Innovation Cluster in Adelaide ( http://www.thewaite.org/waite-partners/wine-innovation-cluster/ ). PM and EP are supported by Biolaffort (Laffort® Research & Development subsidiary) for this project; in addition PM received a grant from Aquitaine Region (Sesam Project) for genome sequencing and QTL analysis.
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Keywords:
Malolactic fermentation, QTL, SSU1, Wine, Yeast-bacteria interactions
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Local EPrints ID: 482769
URI: http://eprints.soton.ac.uk/id/eprint/482769
ISSN: 0175-7598
PURE UUID: 8acb8d2d-646b-4e52-a4e2-eef91adcdded
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Date deposited: 12 Oct 2023 16:42
Last modified: 18 Mar 2024 04:12
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Contributors
Author:
Louise Bartle
Author:
Emilien Peltier
Author:
Joanna F. Sundstrom
Author:
Krista Sumby
Author:
James G. Mitchell
Author:
Vladimir Jiranek
Author:
Philippe Marullo
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