‘TeeBot’: a high throughput robotic fermentation and sampling system

Pellekaan, Nicholas van Holst, Walker, Michelle E., Watson, Tommaso L. and Jiranek, Vladimir (2021) ‘TeeBot’: a high throughput robotic fermentation and sampling system. Fermentation, 7 (4), [205]. (doi:10.3390/fermentation7040205).

Abstract

When fermentation research requires the comparison of many strains or conditions, the major bottleneck is a technical one. Microplate approaches are not able to produce representative fermentative performance due to their inability to truly operate anaerobically, whilst more traditional methods do not facilitate sample density sufficient to assess enough candidates to be consid-ered even medium throughput. Two robotic platforms have been developed that address these tech-nological shortfalls. Both are built on commercially available liquid handling platforms fitted with custom labware. Results are presented detailing fermentation performance as compared to current best practice, i.e., shake flasks fitted with airlocks and sideports. The ‘TeeBot’ is capable sampling from 96 or 384 fermentations in 100 mL or 30 mL volumes, respectively, with airlock sealing and minimal headspace. Sampling and downstream analysis are facilitated by automated liquid han-dling, use of 96‐well sample plate format and temporary cryo‐storage (<0 °C).

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Contributors

Author: Vladimir Jiranek

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