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Apilactobacillus apisilvae sp. nov., Nicolia spurrieriana gen. nov. sp. nov., Bombilactobacillus folatiphilus sp. nov. and Bombilactobacillus thymidiniphilus sp. nov., four new lactic acid bacterial isolates from stingless bees Tetragonula carbonaria and Austroplebeia australis

Apilactobacillus apisilvae sp. nov., Nicolia spurrieriana gen. nov. sp. nov., Bombilactobacillus folatiphilus sp. nov. and Bombilactobacillus thymidiniphilus sp. nov., four new lactic acid bacterial isolates from stingless bees Tetragonula carbonaria and Austroplebeia australis
Apilactobacillus apisilvae sp. nov., Nicolia spurrieriana gen. nov. sp. nov., Bombilactobacillus folatiphilus sp. nov. and Bombilactobacillus thymidiniphilus sp. nov., four new lactic acid bacterial isolates from stingless bees Tetragonula carbonaria and Austroplebeia australis

Four strains, SG5_A10T, SGEP1_A5T, SG4_D2T, and SG4_A1T, were isolated from the honey or homogenate of Australian stingless bee species Tetragonula carbonaria and Austroplebeia australis. Based on 16S rRNA gene phylogeny, core gene phylogenetics, whole genome analyses such as determination of amino acid identity (AAI), cAAI of conserved genes, average nucleotide identity (ANI), and digital DNA–DNA hybridization (dDDH), chemotaxonomic analyses, and the novel isolation sources and unique geography, we propose three new species and one genus with the names Apilactobacillus apisilvae sp. nov. (SG5_A10T = LMG 32133T = NBRC 114991T), Bombilactobacillus thymidiniphilus sp. nov. (SG4_A1T = LMG 32125T = NBRC 114984T), Bombilactobacillus folatiphilus sp. nov. (SG4_D2T = LMG 32126T = NBRC 115004T) and Nicolia spurrieriana sp. nov. (SGEP1_A5T = LMG 32134T = NBRC 114992T). Three out of the four strains were found to be fructophilic, where SG5_A10T and SGEP1_A5T belong to obligately fructophilic lactic acid bacteria, and SG4_D2T representing a new type denoted here as kinetically fructophilic. This study represents the first published lactic acid bacterial species associated with the unique niche of Australian stingless bees.

Apilactobacillus, Austroplebeia australis, Bombilactobacillus, FLAB, Fructobacillus, fructophilic lactic acid bacteria, LAB, stingless bees, sugarbag bees, Tetragonula carbonaria
1466-5026
Oliphant, Scott A.
2e398e47-2147-428b-84ae-2abd6a989121
Watson-Haigh, Nathan S.
94ec3119-96c9-495a-88ec-bb8915cad46b
Sumby, Krista M.
f54b8f1c-c29e-488b-ab5e-dbb9b2576ba9
Gardner, Jennifer
0d95188b-206d-4817-8437-e163351f6e7f
Groom, Scott
32f746ab-f4c7-4ac7-9b80-f0b54ee7daca
Jiranek, Vladimir
8e5a8dfd-f5b2-43e3-928b-11dff324abc7
Oliphant, Scott A.
2e398e47-2147-428b-84ae-2abd6a989121
Watson-Haigh, Nathan S.
94ec3119-96c9-495a-88ec-bb8915cad46b
Sumby, Krista M.
f54b8f1c-c29e-488b-ab5e-dbb9b2576ba9
Gardner, Jennifer
0d95188b-206d-4817-8437-e163351f6e7f
Groom, Scott
32f746ab-f4c7-4ac7-9b80-f0b54ee7daca
Jiranek, Vladimir
8e5a8dfd-f5b2-43e3-928b-11dff324abc7

Oliphant, Scott A., Watson-Haigh, Nathan S., Sumby, Krista M., Gardner, Jennifer, Groom, Scott and Jiranek, Vladimir (2022) Apilactobacillus apisilvae sp. nov., Nicolia spurrieriana gen. nov. sp. nov., Bombilactobacillus folatiphilus sp. nov. and Bombilactobacillus thymidiniphilus sp. nov., four new lactic acid bacterial isolates from stingless bees Tetragonula carbonaria and Austroplebeia australis. International Journal of Systematic and Evolutionary Microbiology, 72 (9), [005588]. (doi:10.1099/ijsem.0.005588).

Record type: Article

Abstract

Four strains, SG5_A10T, SGEP1_A5T, SG4_D2T, and SG4_A1T, were isolated from the honey or homogenate of Australian stingless bee species Tetragonula carbonaria and Austroplebeia australis. Based on 16S rRNA gene phylogeny, core gene phylogenetics, whole genome analyses such as determination of amino acid identity (AAI), cAAI of conserved genes, average nucleotide identity (ANI), and digital DNA–DNA hybridization (dDDH), chemotaxonomic analyses, and the novel isolation sources and unique geography, we propose three new species and one genus with the names Apilactobacillus apisilvae sp. nov. (SG5_A10T = LMG 32133T = NBRC 114991T), Bombilactobacillus thymidiniphilus sp. nov. (SG4_A1T = LMG 32125T = NBRC 114984T), Bombilactobacillus folatiphilus sp. nov. (SG4_D2T = LMG 32126T = NBRC 115004T) and Nicolia spurrieriana sp. nov. (SGEP1_A5T = LMG 32134T = NBRC 114992T). Three out of the four strains were found to be fructophilic, where SG5_A10T and SGEP1_A5T belong to obligately fructophilic lactic acid bacteria, and SG4_D2T representing a new type denoted here as kinetically fructophilic. This study represents the first published lactic acid bacterial species associated with the unique niche of Australian stingless bees.

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More information

Accepted/In Press date: 15 August 2022
Published date: 12 September 2022
Additional Information: Funding Information: V.J. and J.G. are funded by Wine Australia in partnership with the University of Adelaide (UA 1803_2.1). K.S. and V.J. are funded by The Australian Research Council Training Centre for Innovative Wine Production (www.arcwinecentre.org.au; project number IC70100008), which is funded by the Australian Government with additional support from Wine Australia and industry partners. S.O. is a University of Adelaide International Scholarship Holder. The University of Adelaide is a member of the Wine Innovation Cluster (https://www.thewaite.org/waite-partners/wine-innovation-cluster/). Funding Information: V.J. and J.G. are funded by Wine Australia in partnership with the University of Adelaide (UA 1803_2.1). K.S. and V.J. are funded by The Australian Research Council Training Centre for Innovative Wine Production (www.arcwinecentre.org.au; project number IC70100008), which is funded by the Australian Government with additional support from Wine Australia and industry partners. S.O. is a University of Adelaide International Scholarship Holder. The University of Adelaide is a member of the Wine Innovation Cluster (https://www.thewaite.org/waite-partners/wine-innovation-cluster/).We are extraordinarily thankful of Tim Heard of Sugarbag Bees for providing samples of bees and honey, Professor Han Baltussen and Dr Jacqueline Clarke of the University of Adelaide for nomenclature advice and their extensive knowledge of Latin. Lastly, we would like to thank Dr Stijn Wittouck of the University of Antwerp for providing the Apilactobacillus signature genes. Publisher Copyright: © 2022 The Authors.
Keywords: Apilactobacillus, Austroplebeia australis, Bombilactobacillus, FLAB, Fructobacillus, fructophilic lactic acid bacteria, LAB, stingless bees, sugarbag bees, Tetragonula carbonaria

Identifiers

Local EPrints ID: 482787
URI: http://eprints.soton.ac.uk/id/eprint/482787
DOI: doi:10.1099/ijsem.0.005588
ISSN: 1466-5026
PURE UUID: d180988d-f831-4a95-9c0e-4e6c6fe4b5c5
ORCID for Vladimir Jiranek: ORCID iD orcid.org/0000-0002-9775-8963

Catalogue record

Date deposited: 12 Oct 2023 16:44
Last modified: 18 Mar 2024 04:12

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Contributors

Author: Scott A. Oliphant
Author: Nathan S. Watson-Haigh
Author: Krista M. Sumby
Author: Jennifer Gardner
Author: Scott Groom
Author: Vladimir Jiranek ORCID iD

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