Fructilactobacillus cliffordii sp. nov., Fructilactobacillus hinvesii sp. nov., Fructilactobacillus myrtifloralis sp. nov., Fructilactobacillus carniphilus sp. nov. and Fructobacillus americanaquae sp. nov., five novel lactic acid bacteria isolated from insects or flowers of Kangaroo Island, South Australia
Fructilactobacillus cliffordii sp. nov., Fructilactobacillus hinvesii sp. nov., Fructilactobacillus myrtifloralis sp. nov., Fructilactobacillus carniphilus sp. nov. and Fructobacillus americanaquae sp. nov., five novel lactic acid bacteria isolated from insects or flowers of Kangaroo Island, South Australia
Six strains, KI11_D11T, KI4_B1, KI11_C11T, KI16_H9T, KI4_A6T and KI3_B9T, were isolated from insects and flowers on Kangaroo Island, South Australia. On the basis of 16S rRNA gene phylogeny, strains KI11_D11T, KI4_B1, KI11_C11T, KI16_H9T, KI4_A6T were found to be closely related to Fructilactobacillus ixorae Ru20-1T. Due to the lack of a whole genome sequence for this species, whole genome sequencing of Fructilactobacillus ixorae Ru20-1T was undertaken. KI3_B9T was found to be closely related to Fructobacillus tropaeoli F214-1T. Utilizing core gene phylogenetics and whole genome analyses, such as determination of AAI, ANI and dDDH, we propose that these six isolates represent five novel species with the names Fructilactobacillus cliffordii (KI11_ D11T= LMG 32130T = NBRC 114988T), Fructilactobacillus hinvesii (KI11_C11T = LMG 32129T = NBRC 114987T), Fructilactobacillus myrtifloralis (KI16_H9T= LMG 32131T = NBRC 114989T) Fructilactobacillus carniphilus (KI4_A6T = LMG 32127T = NBRC 114985T) and Fructobacillus americanaquae (KI3_B9T = LMG 32124T = NBRC 114983T). Chemotaxonomic analyses detected no fructophilic characters for these strains of member of the genus Fructilactobacillus. KI3_B9T was found to be obligately fructophilic, similarly to its phylogenetic neighbours in the genus Fructobacillus. This study represents the first isolation, to our knowledge, of novel species in the family Lactobacillaceae from the Australian wild.
Apis mellifera, Australian lactic acid bacteria, Blatta orientalis, Calytrix glaberrima, European honey bees, FLAB, Fructilactobacillus, Fructobacillus, fructophilic lactic acid bacteria, Iridomyrmex purpureus, Kangaroo Island, meat ant, Melaleuca gibbosa, oriental cockroach, slender honey-myrtle, smooth fringemyrtle
Oliphant, Scott A.
2e398e47-2147-428b-84ae-2abd6a989121
Watson-Haigh, Nathan S.
94ec3119-96c9-495a-88ec-bb8915cad46b
Sumby, Krista M.
f54b8f1c-c29e-488b-ab5e-dbb9b2576ba9
Gardner, Jennifer M.
0d95188b-206d-4817-8437-e163351f6e7f
Jiranek, Vladimir
8e5a8dfd-f5b2-43e3-928b-11dff324abc7
16 February 2023
Oliphant, Scott A.
2e398e47-2147-428b-84ae-2abd6a989121
Watson-Haigh, Nathan S.
94ec3119-96c9-495a-88ec-bb8915cad46b
Sumby, Krista M.
f54b8f1c-c29e-488b-ab5e-dbb9b2576ba9
Gardner, Jennifer M.
0d95188b-206d-4817-8437-e163351f6e7f
Jiranek, Vladimir
8e5a8dfd-f5b2-43e3-928b-11dff324abc7
Oliphant, Scott A., Watson-Haigh, Nathan S., Sumby, Krista M., Gardner, Jennifer M. and Jiranek, Vladimir
(2023)
Fructilactobacillus cliffordii sp. nov., Fructilactobacillus hinvesii sp. nov., Fructilactobacillus myrtifloralis sp. nov., Fructilactobacillus carniphilus sp. nov. and Fructobacillus americanaquae sp. nov., five novel lactic acid bacteria isolated from insects or flowers of Kangaroo Island, South Australia.
International Journal of Systematic and Evolutionary Microbiology, 73 (2), [005730].
(doi:10.1099/ijsem.0.005730).
Abstract
Six strains, KI11_D11T, KI4_B1, KI11_C11T, KI16_H9T, KI4_A6T and KI3_B9T, were isolated from insects and flowers on Kangaroo Island, South Australia. On the basis of 16S rRNA gene phylogeny, strains KI11_D11T, KI4_B1, KI11_C11T, KI16_H9T, KI4_A6T were found to be closely related to Fructilactobacillus ixorae Ru20-1T. Due to the lack of a whole genome sequence for this species, whole genome sequencing of Fructilactobacillus ixorae Ru20-1T was undertaken. KI3_B9T was found to be closely related to Fructobacillus tropaeoli F214-1T. Utilizing core gene phylogenetics and whole genome analyses, such as determination of AAI, ANI and dDDH, we propose that these six isolates represent five novel species with the names Fructilactobacillus cliffordii (KI11_ D11T= LMG 32130T = NBRC 114988T), Fructilactobacillus hinvesii (KI11_C11T = LMG 32129T = NBRC 114987T), Fructilactobacillus myrtifloralis (KI16_H9T= LMG 32131T = NBRC 114989T) Fructilactobacillus carniphilus (KI4_A6T = LMG 32127T = NBRC 114985T) and Fructobacillus americanaquae (KI3_B9T = LMG 32124T = NBRC 114983T). Chemotaxonomic analyses detected no fructophilic characters for these strains of member of the genus Fructilactobacillus. KI3_B9T was found to be obligately fructophilic, similarly to its phylogenetic neighbours in the genus Fructobacillus. This study represents the first isolation, to our knowledge, of novel species in the family Lactobacillaceae from the Australian wild.
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Published date: 16 February 2023
Additional Information:
Funding Information:
V.J. and J.M.G. are funded by Wine Australia in partnership with the University of Adelaide [UA 1803_2.1]. K.M.S. and V.J. are funded by The Australian Research Council Training Centre for Innovative Wine Production [www.ARCwinecentre.org.au; project number IC70100008], which is funded by the Australian Government with additional support from Wine Australia and industry partners. S.A.O. is a University of Adelaide International Scholarship Holder. Additional project funding was provided by BioLaffort, Floirac, France [UA205016]. The University of Adelaide is a member of the Wine Innovation Cluster (http://www.thewaite.org.waite-partners/wine-innovation-cluster/).
Publisher Copyright:
© 2023 The Authors.
Keywords:
Apis mellifera, Australian lactic acid bacteria, Blatta orientalis, Calytrix glaberrima, European honey bees, FLAB, Fructilactobacillus, Fructobacillus, fructophilic lactic acid bacteria, Iridomyrmex purpureus, Kangaroo Island, meat ant, Melaleuca gibbosa, oriental cockroach, slender honey-myrtle, smooth fringemyrtle
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Local EPrints ID: 482822
URI: http://eprints.soton.ac.uk/id/eprint/482822
ISSN: 1466-5026
PURE UUID: 0f4fbbfc-26e0-4dac-a7a8-6e45b5bafc36
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Date deposited: 12 Oct 2023 16:52
Last modified: 18 Mar 2024 04:12
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Contributors
Author:
Scott A. Oliphant
Author:
Nathan S. Watson-Haigh
Author:
Krista M. Sumby
Author:
Jennifer M. Gardner
Author:
Vladimir Jiranek
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