A liquid metal-polydopamine composite for cell culture and electro-stimulation
A liquid metal-polydopamine composite for cell culture and electro-stimulation
Gallium (Ga) is a low melting point metal in the liquid state in the biological environment which presents a unique combination of fluidity, softness, and metallic electrical and thermal properties. In this work, liquid Ga is proposed as a biocompatible electrode material for cell culture by electro-stimulation since the cytotoxicity of Ga is generally considered low and some Ga compounds have been reported to exhibit anti-bacterial and anti-inflammatory activities. Complementarily, polydopamine (PDA) was coated on liquid Ga to increase the attachment capability of cells on the liquid Ga electrode and provide enhanced biocompatibility. The liquid Ga layer could be readily painted at room temperature on a solid inert substrate, followed by the formation of a nanoscale PDA coating layer resulting in a conformable and biocompatible composite electrode. The PDA layer was shown to coordinate with Ga3+, which is sourced from liquid Ga, providing electrical conductivity in the cell culture medium. The PDA-Ga3+ composite acted as a conductive substrate for advanced electro-stimulation for cell culture methods of representative animal fibroblasts. The cell proliferation was observed to increase by ∼143% as compared to a standard glass coverslip at a low potential of 0.1 V of direct coupling stimulation. This novel PDA-Ga3+ composite has potential applications in cell culture and regenerative medicine.
Animals, Polymers/pharmacology, Biocompatible Materials/pharmacology, Gallium/pharmacology, Cell Culture Techniques
3941-3950
Allioux, Francois Marie
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Merhebi, Salma
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Liu, Li
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Centurion, Franco
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Abbasi, Roozbeh
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Zhang, Chengchen
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Ireland, Jake
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Biazik, Joanna M.
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Mayyas, Mohannad
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Yang, Jiong
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Mousavi, Maedehsadat
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Ghasemian, Mohammad B.
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Tang, Jianbo
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Xie, Wanjie
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Rahim, Md Arifur
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Kalantar-Zadeh, Kourosh
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4 April 2023
Allioux, Francois Marie
06fa4c1f-9d93-4fa4-bff6-2b4b4d497e95
Merhebi, Salma
1d12f9d9-d9b9-4eb6-b328-b9322c4eaada
Liu, Li
f4837a3b-8d3e-48fb-927d-f01c9bf72094
Centurion, Franco
7b4b6572-ddbb-40d1-a79c-4681933fac76
Abbasi, Roozbeh
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Zhang, Chengchen
abc47c06-4b99-4aed-be72-463f211e9dfa
Ireland, Jake
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Biazik, Joanna M.
22e0202e-d477-40ad-9f7a-edc84bd14978
Mayyas, Mohannad
9f9295c4-deda-4289-9cc8-bba31bce5c99
Yang, Jiong
69a8f107-4f74-495f-9944-f15e966ef87c
Mousavi, Maedehsadat
43bca89f-f14f-4c48-95f2-8a3b021945de
Ghasemian, Mohammad B.
10f753c5-c1b6-4537-84a0-9c5ca9dadaf2
Tang, Jianbo
87473905-8e35-48a1-a776-88080bb751b9
Xie, Wanjie
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Rahim, Md Arifur
1a336538-113c-494c-9dd9-04e7f466f323
Kalantar-Zadeh, Kourosh
aded6a64-8612-40b7-aae9-233fbae916a6
Allioux, Francois Marie, Merhebi, Salma, Liu, Li, Centurion, Franco, Abbasi, Roozbeh, Zhang, Chengchen, Ireland, Jake, Biazik, Joanna M., Mayyas, Mohannad, Yang, Jiong, Mousavi, Maedehsadat, Ghasemian, Mohammad B., Tang, Jianbo, Xie, Wanjie, Rahim, Md Arifur and Kalantar-Zadeh, Kourosh
(2023)
A liquid metal-polydopamine composite for cell culture and electro-stimulation.
Journal of Materials Chemistry B, 11 (17), .
(doi:10.1039/d2tb02079c).
Abstract
Gallium (Ga) is a low melting point metal in the liquid state in the biological environment which presents a unique combination of fluidity, softness, and metallic electrical and thermal properties. In this work, liquid Ga is proposed as a biocompatible electrode material for cell culture by electro-stimulation since the cytotoxicity of Ga is generally considered low and some Ga compounds have been reported to exhibit anti-bacterial and anti-inflammatory activities. Complementarily, polydopamine (PDA) was coated on liquid Ga to increase the attachment capability of cells on the liquid Ga electrode and provide enhanced biocompatibility. The liquid Ga layer could be readily painted at room temperature on a solid inert substrate, followed by the formation of a nanoscale PDA coating layer resulting in a conformable and biocompatible composite electrode. The PDA layer was shown to coordinate with Ga3+, which is sourced from liquid Ga, providing electrical conductivity in the cell culture medium. The PDA-Ga3+ composite acted as a conductive substrate for advanced electro-stimulation for cell culture methods of representative animal fibroblasts. The cell proliferation was observed to increase by ∼143% as compared to a standard glass coverslip at a low potential of 0.1 V of direct coupling stimulation. This novel PDA-Ga3+ composite has potential applications in cell culture and regenerative medicine.
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d2tb02079c
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Accepted/In Press date: 2 April 2023
Published date: 4 April 2023
Additional Information:
Funding Information:
This work has been accomplished with the support from the Australian Research Council (ARC) Laureate Fellowship grant (FL180100053). The authors thank the Electron Microscope Unit within the Mark Wainwright Analytical Centre at UNSW Sydney.
Publisher Copyright:
© 2023 The Royal Society of Chemistry.
Keywords:
Animals, Polymers/pharmacology, Biocompatible Materials/pharmacology, Gallium/pharmacology, Cell Culture Techniques
Identifiers
Local EPrints ID: 482916
URI: http://eprints.soton.ac.uk/id/eprint/482916
ISSN: 2050-750X
PURE UUID: 0b8129aa-e8df-4acb-b6e9-8a00c21c9ede
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Date deposited: 17 Oct 2023 16:41
Last modified: 06 Jun 2024 02:19
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Contributors
Author:
Francois Marie Allioux
Author:
Salma Merhebi
Author:
Li Liu
Author:
Franco Centurion
Author:
Roozbeh Abbasi
Author:
Chengchen Zhang
Author:
Jake Ireland
Author:
Joanna M. Biazik
Author:
Mohannad Mayyas
Author:
Jiong Yang
Author:
Maedehsadat Mousavi
Author:
Mohammad B. Ghasemian
Author:
Jianbo Tang
Author:
Wanjie Xie
Author:
Md Arifur Rahim
Author:
Kourosh Kalantar-Zadeh
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