Biochemical and molecular characterization of sucrose and amino acid carriers in Ricinus communis
Biochemical and molecular characterization of sucrose and amino acid carriers in Ricinus communis
The use of energized plasma membrane vesicle preparations from cotyledons and roots of Ricinus communis seedlings is described, and evidence is presented for the existence of plasma membrane H+/sucrose and H+/amino acid symporters. Using fractions isolated from roots, there is evidence for at least two carriers which can transport neutral amino acids and one which can also transport basic amino acids. A method for the solubilization and reconstitution of glutamine transport activity is described. Preliminary results on the molecular characterization of two putative amino acid carriers and a putative sucrose transporter from Ricinus are presented.
carrier, glutamine, plasma membrane, Ricinus communis, sucrose
1211-1216
Williams, L.E.
79ee1856-3732-492b-8ac5-239749c85d9e
Bick, J.A.
953907d5-dc2f-468f-80db-801edb7a75fc
Neelam, A
6a32871d-4fe3-41d9-89d8-bc169249ad92
Weston, K.N.
5b7ca24c-1930-409c-acca-21e4a53f55ef
Hall, J.L.
24cd62e9-4050-4514-9446-5a03949007f4
1 August 1996
Williams, L.E.
79ee1856-3732-492b-8ac5-239749c85d9e
Bick, J.A.
953907d5-dc2f-468f-80db-801edb7a75fc
Neelam, A
6a32871d-4fe3-41d9-89d8-bc169249ad92
Weston, K.N.
5b7ca24c-1930-409c-acca-21e4a53f55ef
Hall, J.L.
24cd62e9-4050-4514-9446-5a03949007f4
Williams, L.E., Bick, J.A., Neelam, A, Weston, K.N. and Hall, J.L.
(1996)
Biochemical and molecular characterization of sucrose and amino acid carriers in Ricinus communis.
Journal of Experimental Botany, 47 (Special issue), .
(doi:10.1093/jxb/47.Special_Issue.1211).
Abstract
The use of energized plasma membrane vesicle preparations from cotyledons and roots of Ricinus communis seedlings is described, and evidence is presented for the existence of plasma membrane H+/sucrose and H+/amino acid symporters. Using fractions isolated from roots, there is evidence for at least two carriers which can transport neutral amino acids and one which can also transport basic amino acids. A method for the solubilization and reconstitution of glutamine transport activity is described. Preliminary results on the molecular characterization of two putative amino acid carriers and a putative sucrose transporter from Ricinus are presented.
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Published date: 1 August 1996
Keywords:
carrier, glutamine, plasma membrane, Ricinus communis, sucrose
Identifiers
Local EPrints ID: 483163
URI: http://eprints.soton.ac.uk/id/eprint/483163
ISSN: 0022-0957
PURE UUID: 4a537cd9-b1ad-4131-95e2-f9c89aa29260
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Date deposited: 25 Oct 2023 17:08
Last modified: 17 Mar 2024 05:17
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Contributors
Author:
J.A. Bick
Author:
A Neelam
Author:
K.N. Weston
Author:
J.L. Hall
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