Techniques for visualization and quantification of primary cilia in chondrocytes
Techniques for visualization and quantification of primary cilia in chondrocytes
Primary cilia regulate and coordinate a variety of cell signaling pathways important in chondrocyte physiology and cartilage development, health, and disease. Despite this, the chondrocyte primary cilium and its associated role in cartilage biology remains poorly understood. Key to elucidating primary cilia structure and function in chondrocytes is the ability to visualize this unique structure. Here we describe materials and methods for immunofluorescence labeling, microscopy, and measurement of chondrocyte primary cilia.
Chondrocyte, Confocal microscopy, Immunofluorescence, Primary cilia, Quantification, Super resolution
157-176
Meng, Huan
c5842e87-23c1-4252-a1c0-45a16c8ae3d0
Thompson, Clare L.
5f801120-d24b-4a1c-bcd1-8499cce49eb2
Coveney, Clarissa R.
254cb939-73c7-462b-b3dc-ea2f38cbd9cc
Wann, Angus K.
f1b0ea2f-dc8a-4588-a9d8-ae462ed0a993
Knight, Martin M.
a3707416-0369-4878-959a-02b09641dd3e
11 November 2022
Meng, Huan
c5842e87-23c1-4252-a1c0-45a16c8ae3d0
Thompson, Clare L.
5f801120-d24b-4a1c-bcd1-8499cce49eb2
Coveney, Clarissa R.
254cb939-73c7-462b-b3dc-ea2f38cbd9cc
Wann, Angus K.
f1b0ea2f-dc8a-4588-a9d8-ae462ed0a993
Knight, Martin M.
a3707416-0369-4878-959a-02b09641dd3e
Meng, Huan, Thompson, Clare L., Coveney, Clarissa R., Wann, Angus K. and Knight, Martin M.
(2022)
Techniques for visualization and quantification of primary cilia in chondrocytes.
In,
Stoddart, Martin J., Della Bella, Elena and Armiento, Angela R.
(eds.)
Cartilage Tissue Engineering.
(Methods in Molecular Biology, 2598)
1 ed.
Humana New York, .
(doi:10.1007/978-1-0716-2839-3_12).
Record type:
Book Section
Abstract
Primary cilia regulate and coordinate a variety of cell signaling pathways important in chondrocyte physiology and cartilage development, health, and disease. Despite this, the chondrocyte primary cilium and its associated role in cartilage biology remains poorly understood. Key to elucidating primary cilia structure and function in chondrocytes is the ability to visualize this unique structure. Here we describe materials and methods for immunofluorescence labeling, microscopy, and measurement of chondrocyte primary cilia.
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More information
e-pub ahead of print date: 11 November 2022
Published date: 11 November 2022
Keywords:
Chondrocyte, Confocal microscopy, Immunofluorescence, Primary cilia, Quantification, Super resolution
Identifiers
Local EPrints ID: 483440
URI: http://eprints.soton.ac.uk/id/eprint/483440
ISSN: 1064-3745
PURE UUID: b06de9df-0f3e-4e25-b9d8-bbb4e19e8460
Catalogue record
Date deposited: 31 Oct 2023 17:30
Last modified: 18 Mar 2024 04:11
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Contributors
Author:
Huan Meng
Author:
Clare L. Thompson
Author:
Clarissa R. Coveney
Author:
Angus K. Wann
Author:
Martin M. Knight
Editor:
Martin J. Stoddart
Editor:
Elena Della Bella
Editor:
Angela R. Armiento
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