Discovery of paralogous GnRH and corazonin signaling systems in an invertebrate chordate
Discovery of paralogous GnRH and corazonin signaling systems in an invertebrate chordate
Gonadotropin-releasing hormone (GnRH) is a key regulator of reproductive function in vertebrates. GnRH is related to the corazonin (CRZ) neuropeptide which influences metabolism and stress responses in insects. Recent evidence suggests that GnRH and CRZ are paralogous and arose by a gene duplication in a common ancestor of bilaterians. Here, we report the identification and complete characterization of the GnRH and CRZ signaling systems in the amphioxus Branchiostoma floridae. We have identified a novel GnRH peptide (YSYSYGFAP-NH2) that specifically activates two GnRH receptors and a CRZ peptide (FTYTHTW-NH2) that activates three CRZ receptors in B. floridae. The latter appear to be promiscuous, as two CRZ receptors can also be activated by GnRH in the physiological range. Hence, there is a potential for cross-talk between these closely related signaling systems. Discovery of both the GnRH and CRZ signaling systems in one of the closest living relatives of vertebrates provides a framework to discover their roles at the transition from invertebrates to vertebrates.
Amino Acid Sequence, Animals, Chordata, Nonvertebrate/metabolism, Gonadotropin-Releasing Hormone/genetics, Invertebrates/genetics, Neuropeptides/genetics, Vertebrates/genetics
Yañez Guerra, Luis Alfonso
cbca947b-bbf0-4b91-96b0-4a126e3b94b6
Zandawala, Meet
3c817de0-f1d0-4351-ac76-33dd54bc3c11
Yañez Guerra, Luis Alfonso
cbca947b-bbf0-4b91-96b0-4a126e3b94b6
Zandawala, Meet
3c817de0-f1d0-4351-ac76-33dd54bc3c11
Yañez Guerra, Luis Alfonso and Zandawala, Meet
(2023)
Discovery of paralogous GnRH and corazonin signaling systems in an invertebrate chordate.
Genome Biology and Evolution, 15 (7), [evad108].
(doi:10.1093/gbe/evad108).
Abstract
Gonadotropin-releasing hormone (GnRH) is a key regulator of reproductive function in vertebrates. GnRH is related to the corazonin (CRZ) neuropeptide which influences metabolism and stress responses in insects. Recent evidence suggests that GnRH and CRZ are paralogous and arose by a gene duplication in a common ancestor of bilaterians. Here, we report the identification and complete characterization of the GnRH and CRZ signaling systems in the amphioxus Branchiostoma floridae. We have identified a novel GnRH peptide (YSYSYGFAP-NH2) that specifically activates two GnRH receptors and a CRZ peptide (FTYTHTW-NH2) that activates three CRZ receptors in B. floridae. The latter appear to be promiscuous, as two CRZ receptors can also be activated by GnRH in the physiological range. Hence, there is a potential for cross-talk between these closely related signaling systems. Discovery of both the GnRH and CRZ signaling systems in one of the closest living relatives of vertebrates provides a framework to discover their roles at the transition from invertebrates to vertebrates.
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evad108
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Accepted/In Press date: 7 June 2023
e-pub ahead of print date: 9 June 2023
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Funding Information:
The authors would like to thank Dr Theresa McKim (University Hospital Würzburg) and Irina Wenzel (University of Würzburg) for helpful feedback during preparation of this paper. This work was supported by funding from the University of Würzburg (awarded to M.Z.) and the BBSRC Discovery fellowship (BB/W010305/1 awarded to L.A.Y.G.). This paper was typeset with the bioRxiv word template by @Chrelli: https://github.com/chrelli/bioRxiv-word-template .
Keywords:
Amino Acid Sequence, Animals, Chordata, Nonvertebrate/metabolism, Gonadotropin-Releasing Hormone/genetics, Invertebrates/genetics, Neuropeptides/genetics, Vertebrates/genetics
Identifiers
Local EPrints ID: 483449
URI: http://eprints.soton.ac.uk/id/eprint/483449
ISSN: 1759-6653
PURE UUID: dabd9d45-f93e-4fab-9e62-c0bb7c4c2bac
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Date deposited: 31 Oct 2023 17:34
Last modified: 18 Mar 2024 04:15
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Author:
Luis Alfonso Yañez Guerra
Author:
Meet Zandawala
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