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Permeability barrier properties of oral keratinocyte cultures: a model of intact human oral mucosa

Record type: Article

OBJECTIVES: The aim of this study was to establish whether an in vitro model of human oral mucosa had similar permeability characteristics to normal oral mucosa. Such a model would have considerable value as an alternative to the use of mucosal biopsies in studies of transmucosal drug delivery.
MATERIALS AND METHODS: Keratinocytes obtained from buccal mucosa, hard palate and abdominal skin were seeded onto inert collagen membranes (Cellagen Discs) or dead deepidermised dermis (DDED) and grown either as submerged or air-liquid interface cultures. Subsequently the ultrastructural characteristics, permeability to water and barrier lipid content of the epithelial cultures were assessed and compared with samples of intact mucosa and skin.
RESULTS: All the cultures stratified into multilayered epithelia and displayed features of differentiation including tonofilaments, desmosomes and membrane coating granules. The permeability characteristics and barrier lipid content of the oral mucosal cultures resembled those of intact mucosa. By contrast, pidermal keratinocytes failed to produce a permeability barrier comparable with that of skin and had low levels of barrier associated lipids.
CONCLUSIONS: Cultures of human oral mucosal keratinocytes obtained from healthy adults develop similar permeability properties and barrier lipid composition to their site of origin. This model system may be useful for the evaluation of local and systemic oral mucosal drug delivery.

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Citation

Selvaratnam, L., Cruchley, A.T., Navsaria, H., Wertz, P.W., Hagi-Pavli, E.P., Leigh, I.M., Squier, C.A. and Williams, D.M. (2001) Permeability barrier properties of oral keratinocyte cultures: a model of intact human oral mucosa Oral Diseases, 7, (4), pp. 252-258. (doi:10.1034/j.1601-0825.2001.70409.x).

More information

Published date: July 2001

Identifiers

Local EPrints ID: 48432
URI: http://eprints.soton.ac.uk/id/eprint/48432
ISSN: 1354-523X
PURE UUID: 0c1964f3-a95a-43f3-8e7d-e5014769dde8

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Date deposited: 21 Sep 2007
Last modified: 17 Jul 2017 14:59

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Contributors

Author: L. Selvaratnam
Author: A.T. Cruchley
Author: H. Navsaria
Author: P.W. Wertz
Author: E.P. Hagi-Pavli
Author: I.M. Leigh
Author: C.A. Squier
Author: D.M. Williams

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