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Femoral neck width genetic risk score is a novel independent risk factor for hip fractures

Femoral neck width genetic risk score is a novel independent risk factor for hip fractures
Femoral neck width genetic risk score is a novel independent risk factor for hip fractures
Femoral neck width (FNW) derived from dual energy X-ray absorptiometry (DXA) scans may provide a useful adjunct to hip fracture prediction, by providing depth information missing from bone mineral density (BMD) measurements. Therefore, we investigated whether FNW is related to hip fracture risk independently of FN-BMD, using a genetic approach. FNW was derived from points automatically placed on the proximal femur using hip DXA scans from 38,150 individuals (mean age 63.8 years, 48.0% males) in UK Biobank (UKB). GWAS identified 71 independent genome-wide significant FNW SNPs, comprising genes involved in cartilage differentiation, hedgehog, skeletal development, in contrast to SNPs identified by FN-BMD GWAS which primarily comprised runx1/Wnt signalling genes (MAGMA gene set analyses). FNW and FN-BMD SNPs were used to generate genetic instruments for multivariable Mendelian randomisation (MVMR). Greater genetically determined FNW increased risk of all hip fractures (OR 1.53; 95% CI 1.29-1.82 per SD increase) and femoral neck fractures (OR 1.58;1.30-1.92), but not trochanteric or forearm fractures. In contrast, greater genetically determined FN-BMD decreased fracture risk at all four sites. FNW and FN-BMD SNPs were also used to generate genetic risk scores (GRSs), which were examined in relation to incident hip fracture in UKB (excluding the FNW GWAS population; n=338742, 3222 cases) using a Cox proportional hazards model. FNW GRS was associated with increased risk of all incident hip fractures (HR 1.08;1.05-1.12) and femoral neck fractures (HR 1.10;1.06-1.15), but not trochanteric fractures, whereas FN-BMD GRS was associated with reduced risk of all hip fracture types. We conclude that the underlying biology regulating FNW and FN-BMD differs, and that DXA-derived FNW is causally related to hip fractures independently of FN-BMD, adding information beyond FN-BMD for hip fracture prediction. Hence, FNW derived from DXA analyses or a FNW GRS may contribute clinically useful information beyond FN-BMD for hip fracture prediction.
0884-0431
Tobias, Jonathan H.
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Nethander, Maria
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Faber, Benjamin G.
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Heppenstall, Sophie V.
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Ebsim, Raja
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Cootes, Tim
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Lindner, Claudia
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Saunders, Fiona R.
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Gregory, Jenny S.
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Aspden, Richard M.
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Harvey, Nicholas C.
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Kemp, John P.
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Frysz, Monika
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Ohlsson, Claes
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Tobias, Jonathan H.
514342d7-3491-4a7b-bbeb-b00dcf244daa
Nethander, Maria
2047d217-51ce-4e43-b2a8-9568a6043380
Faber, Benjamin G.
85a38e7f-74a4-4ba7-a985-a1cff3392ed0
Heppenstall, Sophie V.
e45f87f8-e929-46b7-8de6-9bc7328e0e1f
Ebsim, Raja
fa3d2f2c-9d77-4b95-b0ff-c34b57142381
Cootes, Tim
f82f878a-ab1d-426c-9510-afa3f6de7aef
Lindner, Claudia
03ee5726-0741-4170-8375-659292641028
Saunders, Fiona R.
a51cc79d-0928-4ab6-a479-3972c974670b
Gregory, Jenny S.
6995d8fa-b32b-4f7c-aa15-8146acb4fd67
Aspden, Richard M.
71d1c790-5d9f-40b4-9130-bf7781b0e0dd
Harvey, Nicholas C.
ce487fb4-d360-4aac-9d17-9466d6cba145
Kemp, John P.
6ada3f84-7fb8-4393-b096-13f1c2acfc87
Frysz, Monika
bda9e219-ca28-43e4-babd-81f2d91ca3e4
Ohlsson, Claes
98168752-6ef1-40c6-b873-9361cc99358b

Tobias, Jonathan H., Nethander, Maria, Faber, Benjamin G., Heppenstall, Sophie V., Ebsim, Raja, Cootes, Tim, Lindner, Claudia, Saunders, Fiona R., Gregory, Jenny S., Aspden, Richard M., Harvey, Nicholas C., Kemp, John P., Frysz, Monika and Ohlsson, Claes (2023) Femoral neck width genetic risk score is a novel independent risk factor for hip fractures. Journal of Bone and Mineral Research. (In Press)

Record type: Article

Abstract

Femoral neck width (FNW) derived from dual energy X-ray absorptiometry (DXA) scans may provide a useful adjunct to hip fracture prediction, by providing depth information missing from bone mineral density (BMD) measurements. Therefore, we investigated whether FNW is related to hip fracture risk independently of FN-BMD, using a genetic approach. FNW was derived from points automatically placed on the proximal femur using hip DXA scans from 38,150 individuals (mean age 63.8 years, 48.0% males) in UK Biobank (UKB). GWAS identified 71 independent genome-wide significant FNW SNPs, comprising genes involved in cartilage differentiation, hedgehog, skeletal development, in contrast to SNPs identified by FN-BMD GWAS which primarily comprised runx1/Wnt signalling genes (MAGMA gene set analyses). FNW and FN-BMD SNPs were used to generate genetic instruments for multivariable Mendelian randomisation (MVMR). Greater genetically determined FNW increased risk of all hip fractures (OR 1.53; 95% CI 1.29-1.82 per SD increase) and femoral neck fractures (OR 1.58;1.30-1.92), but not trochanteric or forearm fractures. In contrast, greater genetically determined FN-BMD decreased fracture risk at all four sites. FNW and FN-BMD SNPs were also used to generate genetic risk scores (GRSs), which were examined in relation to incident hip fracture in UKB (excluding the FNW GWAS population; n=338742, 3222 cases) using a Cox proportional hazards model. FNW GRS was associated with increased risk of all incident hip fractures (HR 1.08;1.05-1.12) and femoral neck fractures (HR 1.10;1.06-1.15), but not trochanteric fractures, whereas FN-BMD GRS was associated with reduced risk of all hip fracture types. We conclude that the underlying biology regulating FNW and FN-BMD differs, and that DXA-derived FNW is causally related to hip fractures independently of FN-BMD, adding information beyond FN-BMD for hip fracture prediction. Hence, FNW derived from DXA analyses or a FNW GRS may contribute clinically useful information beyond FN-BMD for hip fracture prediction.

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JT21.9.23.Rev2.2-clean - Accepted Manuscript
Available under License Creative Commons Attribution.
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Accepted/In Press date: 31 October 2023

Identifiers

Local EPrints ID: 484337
URI: http://eprints.soton.ac.uk/id/eprint/484337
ISSN: 0884-0431
PURE UUID: 29367ee9-3f98-4673-8707-733c4964bdba
ORCID for Nicholas C. Harvey: ORCID iD orcid.org/0000-0002-8194-2512

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Date deposited: 15 Nov 2023 18:13
Last modified: 18 Mar 2024 05:02

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Contributors

Author: Jonathan H. Tobias
Author: Maria Nethander
Author: Benjamin G. Faber
Author: Sophie V. Heppenstall
Author: Raja Ebsim
Author: Tim Cootes
Author: Claudia Lindner
Author: Fiona R. Saunders
Author: Jenny S. Gregory
Author: Richard M. Aspden
Author: John P. Kemp
Author: Monika Frysz
Author: Claes Ohlsson

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