Dual function of OmpM as outer membrane tether and nutrient uptake channel in diderm Firmicutes

Accepted/In Press date: 16 October 2023

e-pub ahead of print date: 6 November 2023

Published date: 6 November 2023

Additional Information: Funding Information: We thank the University of York (UK) electron cryo-microscopy facility for access to instrumentation and support. We acknowledge the Diamond Light Source for I03 beamline access (proposal mx-24948) and support. We thank David Staunton (University of Oxford) for carrying out the dynamic scanning calorimetry experiments. This work was supported by a Wellcome Trust Investigator award (214222/Z/18/Z) to B.v.d.B., providing salary support to A.S. and Y.Z. C.B., S.G., J.W., and R.E.S. were supported by funding from the French National Research Agency (ANR) (no. Fir-OM ANR-16-CE12-0010), the Institut Pasteur Programmes Transversaux de Recherche (no. PTR 39-16), and the French government Investissement d’Avenir Program, Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases (grant no. ANR-10-LABX-62-IBEID). The authors acknowledge the use of the IRIDIS High Performance Computing Facility, and associated support services at the University of Southampton, in the completion of this work. K.E.N. was supported by a Ph.D. Studentship from the Engineering and Physical Sciences Research Council (Project Number: 2446840), and S.K. by an EPSRC established Career Fellowship (EPSRC grant no. EP/V030779/1). Funding Information: We thank the University of York (UK) electron cryo-microscopy facility for access to instrumentation and support. We acknowledge the Diamond Light Source for I03 beamline access (proposal mx-24948) and support. We thank David Staunton (University of Oxford) for carrying out the dynamic scanning calorimetry experiments. This work was supported by a Wellcome Trust Investigator award (214222/Z/18/Z) to B.v.d.B., providing salary support to A.S. and Y.Z. C.B., S.G., J.W., and R.E.S. were supported by funding from the French National Research Agency (ANR) (no. Fir-OM ANR-16-CE12-0010), the Institut Pasteur Programmes Transversaux de Recherche (no. PTR 39-16), and the French government Investissement d’Avenir Program, Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases (grant no. ANR-10-LABX-62-IBEID). The authors acknowledge the use of the IRIDIS High Performance Computing Facility, and associated support services at the University of Southampton, in the completion of this work. K.E.N. was supported by a Ph.D. Studentship from the Engineering and Physical Sciences Research Council (Project Number: 2446840), and S.K. by an EPSRC established Career Fellowship (EPSRC grant no. EP/V030779/1). © 2023. The Author(s).

Keywords: Firmicutes, Cell Membrane/metabolism, Cell Wall/metabolism, Bacteria/metabolism, Gram-Negative Bacteria/metabolism, Nutrients, Bacterial Outer Membrane Proteins/metabolism

Local EPrints ID: 484810

URI: http://eprints.soton.ac.uk/id/eprint/484810

DOI: doi:10.1038/s41467-023-42601-y

ISSN: 2041-1723

PURE UUID: 9706eaeb-3494-426c-bc6c-2d154aa224c6

ORCID for Syma Khalid: orcid.org/0000-0002-3694-5044

Date deposited: 22 Nov 2023 17:32

Last modified: 13 Apr 2024 01:41