Improving tuberculosis treatment using mid-infrared spectroscopy for bedside therapeutic drug monitoring
Improving tuberculosis treatment using mid-infrared spectroscopy for bedside therapeutic drug monitoring
Rifampicin is an antimicrobial drug used to treat tuberculosis. The deterioration of a tuberculosis patient on rifampicin is a serious event with several possible causes. Rapid bedside measurement of rifampicin would enable clinicians to determine if patient deterioration was due to subtherapeutic levels and quickly correct the dosing. It would also support personalised dosing to maximise antimicrobial effectiveness whilst minimising side effects. The optimum therapeutic concentration range is 8-24 mg/L. We report ATR-FTIR spectroscopy data for the detection of rifampicin for bedside therapeutic drug monitoring (TDM). We demonstrate a limit of detection of 0.46 mg/L from 20 μL spiked whole blood samples. Using whole blood directly enables bedside measurements because it does not require centrifugation and pipetting to extract plasma, which are generally performed in a central laboratory. The absorption-concentration response had good linearity (R2 = 0.998) up to the highest measured concentration of 100 mg/L. We apply this data to the design of a miniaturised mid-infrared sensor for TDM using silicon photonics. We present an analysis of the optimum interaction length for an evanescent waveguide sensor using the absorption of rifampicin and a numerical model to quantify the contributions of different system and device noise sources. These sensors can be made more sensitive than their benchtop equivalent because of the enhanced evanescent electric field strength and the increased power spectral density of tunable quantum cascade lasers.
ATR-FTIR spectroscopy, biosensors, mid-infrared applications, point-of-care, therapeutic drug monitoring, tuberculosis, waveguide sensors
Rowe, David J.
a0e0fe82-5e29-42b8-b370-5236a722f015
Owens, Daniel R.
3a66adc3-6a24-4eca-a171-1880a8372fe6
Nedeljković, Miloš
b64e21c2-1b95-479d-a35c-3456dff8c796
Stirling, Callum J.
2b1db9ce-c03c-4380-b371-a92748d7c4b9
Cathie, Katrina
ec8dd2eb-0be9-4567-b87d-72eadf5a254b
Parker, Suzanne L.
f6c0e196-89ad-4ab2-971f-f579a57615a9
Faust, Saul N.
f97df780-9f9b-418e-b349-7adf63e150c1
Mashanovich, Goran Z.
c806e262-af80-4836-b96f-319425060051
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
13 March 2023
Rowe, David J.
a0e0fe82-5e29-42b8-b370-5236a722f015
Owens, Daniel R.
3a66adc3-6a24-4eca-a171-1880a8372fe6
Nedeljković, Miloš
b64e21c2-1b95-479d-a35c-3456dff8c796
Stirling, Callum J.
2b1db9ce-c03c-4380-b371-a92748d7c4b9
Cathie, Katrina
ec8dd2eb-0be9-4567-b87d-72eadf5a254b
Parker, Suzanne L.
f6c0e196-89ad-4ab2-971f-f579a57615a9
Faust, Saul N.
f97df780-9f9b-418e-b349-7adf63e150c1
Mashanovich, Goran Z.
c806e262-af80-4836-b96f-319425060051
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Rowe, David J., Owens, Daniel R., Nedeljković, Miloš, Stirling, Callum J., Cathie, Katrina, Parker, Suzanne L., Faust, Saul N., Mashanovich, Goran Z. and Wilkinson, James S.
(2023)
Improving tuberculosis treatment using mid-infrared spectroscopy for bedside therapeutic drug monitoring.
Reed, Graham T. and Knights, Andrew P.
(eds.)
In Silicon Photonics XVIII.
vol. 12426,
SPIE.
7 pp
.
(doi:10.1117/12.2649135).
Record type:
Conference or Workshop Item
(Paper)
Abstract
Rifampicin is an antimicrobial drug used to treat tuberculosis. The deterioration of a tuberculosis patient on rifampicin is a serious event with several possible causes. Rapid bedside measurement of rifampicin would enable clinicians to determine if patient deterioration was due to subtherapeutic levels and quickly correct the dosing. It would also support personalised dosing to maximise antimicrobial effectiveness whilst minimising side effects. The optimum therapeutic concentration range is 8-24 mg/L. We report ATR-FTIR spectroscopy data for the detection of rifampicin for bedside therapeutic drug monitoring (TDM). We demonstrate a limit of detection of 0.46 mg/L from 20 μL spiked whole blood samples. Using whole blood directly enables bedside measurements because it does not require centrifugation and pipetting to extract plasma, which are generally performed in a central laboratory. The absorption-concentration response had good linearity (R2 = 0.998) up to the highest measured concentration of 100 mg/L. We apply this data to the design of a miniaturised mid-infrared sensor for TDM using silicon photonics. We present an analysis of the optimum interaction length for an evanescent waveguide sensor using the absorption of rifampicin and a numerical model to quantify the contributions of different system and device noise sources. These sensors can be made more sensitive than their benchtop equivalent because of the enhanced evanescent electric field strength and the increased power spectral density of tunable quantum cascade lasers.
Text
124260G
- Version of Record
Available under License Other.
More information
Published date: 13 March 2023
Venue - Dates:
Silicon Photonics XVIII 2023, , San Francisco, United States, 2023-01-30 - 2023-02-01
Keywords:
ATR-FTIR spectroscopy, biosensors, mid-infrared applications, point-of-care, therapeutic drug monitoring, tuberculosis, waveguide sensors
Identifiers
Local EPrints ID: 484909
URI: http://eprints.soton.ac.uk/id/eprint/484909
ISSN: 0277-786X
PURE UUID: 9278bf33-10ac-4ddc-bfd6-135f95cd18c3
Catalogue record
Date deposited: 24 Nov 2023 17:35
Last modified: 18 Mar 2024 02:31
Export record
Altmetrics
Contributors
Author:
Daniel R. Owens
Author:
Callum J. Stirling
Author:
Katrina Cathie
Author:
Suzanne L. Parker
Editor:
Graham T. Reed
Editor:
Andrew P. Knights
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
