Cattle killer immunoglobulin-like receptor expression on leukocyte subsets suggests functional divergence compared to humans
Cattle killer immunoglobulin-like receptor expression on leukocyte subsets suggests functional divergence compared to humans
Cattle, sheep, and goats are the only species outside primates known to have an expanded and diversified family of killer immunoglobulin-like receptors (KIR). Primate KIR are expressed on the surface of NK and T cells and bind MHC-I to control activation. However, the surface expression, ligands and function of bovid KIR remain unknown. Cattle botaKIR2DL1 is the only functional KIR of the same DL-lineage as the expanded KIR in primates and we examined if leukocyte expression patterns were consistent with human. We raised a specific mouse anti-botaKIR2DL1 monoclonal antibody and assessed its utility in flow cytometry, ELISA, and western blot. Unlike primates, cattle DL-lineage KIR (botaKIR2DL1) is present on B cells and monocytes in addition to T cells and low-level expression on NK cells. Expression decreases after in vitro PBMC stimulation with IL-2. This suggests that botaKIR2DL1 has different functions, and potentially ligands, compared to primate KIR.
Animals, Cattle, Goats, Humans, Immunosuppressive Agents, Leukocytes, Leukocytes, Mononuclear, Ligands, Mice, Monocytes, Receptors, KIR, Sheep, Antibody, KIR2DL1, KIR, NK cell, MHC-I
Hay, Abigail L.
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Birch, James
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Ellis, Shirley
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Burns, Daniel
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Mansour, Salah
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Khakoo, Salim I.
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Hammond, John A.
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September 2023
Hay, Abigail L.
677994a5-72b8-4594-9c9a-f1afb0f4106e
Birch, James
c4c55cc9-54a8-41fa-bf84-beb0c953f1fa
Ellis, Shirley
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Burns, Daniel
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Mansour, Salah
4aecba5a-8387-4f7b-b766-0a9c309ccb8b
Khakoo, Salim I.
6c16d2f5-ae80-4d9b-9100-6bfb34ad0273
Hammond, John A.
9a151aa4-a8c5-4298-a780-5d41fce13c5a
Hay, Abigail L., Birch, James, Ellis, Shirley, Burns, Daniel, Mansour, Salah, Khakoo, Salim I. and Hammond, John A.
(2023)
Cattle killer immunoglobulin-like receptor expression on leukocyte subsets suggests functional divergence compared to humans.
Veterinary immunology and immunopathology, 263, [110646].
(doi:10.1016/j.vetimm.2023.110646).
Abstract
Cattle, sheep, and goats are the only species outside primates known to have an expanded and diversified family of killer immunoglobulin-like receptors (KIR). Primate KIR are expressed on the surface of NK and T cells and bind MHC-I to control activation. However, the surface expression, ligands and function of bovid KIR remain unknown. Cattle botaKIR2DL1 is the only functional KIR of the same DL-lineage as the expanded KIR in primates and we examined if leukocyte expression patterns were consistent with human. We raised a specific mouse anti-botaKIR2DL1 monoclonal antibody and assessed its utility in flow cytometry, ELISA, and western blot. Unlike primates, cattle DL-lineage KIR (botaKIR2DL1) is present on B cells and monocytes in addition to T cells and low-level expression on NK cells. Expression decreases after in vitro PBMC stimulation with IL-2. This suggests that botaKIR2DL1 has different functions, and potentially ligands, compared to primate KIR.
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Accepted/In Press date: 21 August 2023
e-pub ahead of print date: 22 August 2023
Published date: September 2023
Additional Information:
Funding Information:
We acknowledge the Pirbright Science Technology Platforms’ support from the Immunological Toolbox and Flow Cytometry. This work was supported by funding from the UK Research and Innovation—Biotechnology and Biological Sciences Research Council (UKRI-BBSRC) (grants BBS/E/I/00001410, BBS/E/I/00007030, BBS/E/I/00007031, BBS/E/I/00007037, BBS/E/I/00007038, and BBS/E/I/00007039 ). The PhD studentship awarded to Abigail Hay was co-funded by The Pirbright Institute and Vice-Chancellor’s studentship from the University of Southampton. The transfection protocol for the ExpiHEK293T cell line was acquired from Rosalind Franklin Institute.
Funding Information:
We acknowledge the Pirbright Science Technology Platforms’ support from the Immunological Toolbox and Flow Cytometry. This work was supported by funding from the UK Research and Innovation—Biotechnology and Biological Sciences Research Council (UKRI-BBSRC) (grants BBS/E/I/00001410, BBS/E/I/00007030, BBS/E/I/00007031, BBS/E/I/00007037, BBS/E/I/00007038, and BBS/E/I/00007039). The PhD studentship awarded to Abigail Hay was co-funded by The Pirbright Institute and Vice-Chancellor's studentship from the University of Southampton. The transfection protocol for the ExpiHEK293T cell line was acquired from Rosalind Franklin Institute.
Publisher Copyright:
© 2023 The Authors
Keywords:
Animals, Cattle, Goats, Humans, Immunosuppressive Agents, Leukocytes, Leukocytes, Mononuclear, Ligands, Mice, Monocytes, Receptors, KIR, Sheep, Antibody, KIR2DL1, KIR, NK cell, MHC-I
Identifiers
Local EPrints ID: 484923
URI: http://eprints.soton.ac.uk/id/eprint/484923
ISSN: 0165-2427
PURE UUID: 2db804b4-f9c9-4b50-9481-8ce3c4cb73a9
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Date deposited: 24 Nov 2023 17:38
Last modified: 06 Jun 2024 01:46
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Author:
Abigail L. Hay
Author:
James Birch
Author:
Shirley Ellis
Author:
Daniel Burns
Author:
John A. Hammond
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