Veluthandath, Aneesh Vincent, Ahmed, Waseem, Madens, Jens, Clark, Howard W., Postle, Anthony D., Wilkinson, James S. and Murugan, Ganapathy Senthil (2023) Quantification of lung surfactant lipid (dipalmitoylphosphatidylcholine/sphingomyelin) ratio in binary liposomes using Raman spectroscopy. Journal of Raman Spectroscopy. (doi:10.1002/jrs.6631).
Abstract
Early diagnosis of neonatal respiratory distress syndrome (nRDS) is important
in reducing the mortality of preterm babies. Knowledge of the ratio of two
components of lung surfactant, dipalmitoylphosphatidylcholine (DPPC), and
sphingomyelin (SM) can be used as biomarkers of lung maturity and inform
treatment. Raman spectroscopy is a powerful tool to analyze vibrational spectra
of organic molecules which requires only limited sample preparation steps
and, unlike IR spectroscopy, is not masked by water absorption. In this paper,
we explore the potential of using Raman spectroscopy as a tool to estimate the
ratio of DPPC and SM from aqueous vesicles of binary mixture of DPPC and
SM. We demonstrate that the ratio of DPPC and SM can be estimated by estimating the ratio of intensity of C O stretch of DPPC and C C stretch of SM
as well as C O stretch of DPPC and amide I of SM. Further, we employ a partial
least squares regression (PLSR) model to automate the estimation and
demonstrate that PLSR method can predict the DPPC and SM ratio with an R2
value of 0.968.
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