Proteomic response to phosphorus deficiency and aluminum stress of three aluminum-tolerant phosphobacteria isolated from acidic soils

Aluminum (Al)-tolerant phosphobacteria enhance plant growth in acidic soils by improving Al complexing and phosphorus (P) availability. However, the impact of Al stress and P deficiency on bacterial biochemistry and physiology remains unclear. We investigated the single and mutual effects of Al stress (10 mM) and P deficiency (0.05 mM) on the proteome of three aluminum-tolerant phosphobacteria: Enterobacter sp. 198, Enterobacter sp. RJAL6, and Klebsiella sp. RCJ4. Cultivated under varying conditions, P deficiency upregulated P metabolism proteins while Al exposure downregulated iron-sulfur and heme-containing proteins and upregulated iron acquisition proteins. This demonstrated that Al influence on iron homeostasis and bacterial central metabolism. This study offers crucial insights into bacterial behavior in acidic soils, benefiting the development of bioinoculants for crops facing Al toxicity and P deficiency. This investigation marks the first proteomic study on the interaction between high Al and P deficiency in acid soils-adapted bacteria.

Bacteriology, Microbiology, proteomics, soil science

10.1016/j.isci.2023.107910

2589-0042

Barra, Patricio Javier

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Duran, Paola

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Delgado, Mabel

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Viscardi, Sharon

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Claverol, Stéphane

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Larama, Giovanni

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Dumont, Marc

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Mora, María de la Luz

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20 October 2023

Barra, Patricio Javier

d2b10ed5-6e75-42b5-9793-af1c7b249ac2

Duran, Paola

778b7a89-74b0-4f6d-a1f4-1ff30f91cffb

Delgado, Mabel

9a92ec9f-7bdd-4da4-ac5e-ce767149cb31

Viscardi, Sharon

a98c30e0-0dad-47b9-bbbb-bfa0bfd2ca55

Claverol, Stéphane

3fdb057a-5238-4b71-8a21-f1e99147c978

Larama, Giovanni

f172773d-a79b-4883-aca5-08ae4bb0db84

Dumont, Marc

afd9f08f-bdbb-4cee-b792-1a7f000ee511

Mora, María de la Luz

8a6e5474-5d20-423c-a194-5d0a6358ddee

Barra, Patricio Javier, Duran, Paola, Delgado, Mabel, Viscardi, Sharon, Claverol, Stéphane, Larama, Giovanni, Dumont, Marc and Mora, María de la Luz (2023) Proteomic response to phosphorus deficiency and aluminum stress of three aluminum-tolerant phosphobacteria isolated from acidic soils. iScience, 26 (10), [107910]. (doi:10.1016/j.isci.2023.107910).

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Accepted/In Press date: 11 September 2023

e-pub ahead of print date: 14 September 2023

Published date: 20 October 2023

Additional Information: Funding Information: This study was financed by the Agencia Nacional de Investigación y Desarrollo (ANID) of Chilean government through FONDECYT Postdoctoral Project No. 3170629 (PJB); FONDECYT initiation project No. 11200377 (PJB) and FONDECYT Regular Projects No. 1210684 (M Delgado); 1201196 (PD) and 1230084 (MLM). This study was also funding by Proyecto REDES ETAPA INICIAL, Convocatoria 2017, REDI170334 (PJB), by Concurso Anillos de Investigación en Áreas Temáticas, ANID ATE220038 (PD, MD, PJB) and by Universidad de La Frontera (DiUFRO), Proyectos de Investigación Vinculados a la Red Nexer No. DNX22-0009 (PJB). The authors acknowledge to Scientific and Technological Bioresource Nucleus of Universidad de La Frontera (BIOREN–UFRO) and Service Management Analytical Research and Training Center (SmartC-BIOREN). The authors also acknowledge to the supercomputing infrastructure of Soroban (SATREPS MACH – JPM/JSA1705), Centro de Modelación y Computación Científica, Universidad de La Frontera, Temuco. Conceptualization, P.J.B. P.D. M. Delgado and M.L.M.; Methodology, P.J.B. S.V. and S.C.; Formal Analysis, S.C. and G.L.; Investigation, P.J.B. S.V. S.C. and G. L.; Resources, P.J.B. P.D. M. Delgado, S.C. and M.L.M.; Data Curation, P.J.B. S.C. and G. L. Writing—Original Draft, P.J.B. P.D. M. Delgado, S.C. and M.D.; Writing—Review & Editing, P.J.B. P.D. M. Delgado, S.C. M.D. and M.L.M.; Visualization, P.J.B. and G.L.; Supervision, P.J.B. and M.L.M. Project Administration, P.J.B. Funding Acquisition, P.J.B. P.D. M. Delgado and M.L.M. The authors declare no competing interests. We support inclusive, diverse, and equitable conduct of research. Funding Information: This study was financed by the Agencia Nacional de Investigación y Desarrollo (ANID) of Chilean government through FONDECYT Postdoctoral Project No. 3170629 (PJB); FONDECYT initiation project No. 11200377 (PJB) and FONDECYT Regular Projects No. 1210684 (M Delgado); 1201196 (PD) and 1230084 (MLM). This study was also funding by Proyecto REDES ETAPA INICIAL , Convocatoria 2017 , REDI170334 (PJB), by Concurso Anillos de Investigación en Áreas Temáticas , ANID ATE220038 (PD, MD, PJB) and by Universidad de La Frontera (DiUFRO), Proyectos de Investigación Vinculados a la Red Nexer No. DNX22-0009 (PJB). Publisher Copyright: © 2023 The Authors

Keywords: Bacteriology, Microbiology, proteomics, soil science

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