Cytotoxic CD4+ tissue-resident memory T cells are associated with asthma severity

Accepted/In Press date: 18 September 2023

e-pub ahead of print date: 20 October 2023

Published date: 8 December 2023

Additional Information: Funding Information: We thank Dr. C. Kim, Dr. D. Hinz, members of the flow cytometry core facility at La Jolla Institute for Immunology, and the members of the Vijayanand laboratory, for scientific support. We also thank all donors for their charitable contribution to academic research. This work was supported by NIH research grants R01HL114093 and U19-AI070535 (P.V.) and equipment grants (S10RR027366 - BD FACSAria II, and S10OD025052 - Illumina Novaseq 6000); the William K. Bowes, Jr. Foundation (P.V.); and BioLegend – BioLegend Fellow (S.H.-M.). The WATCH study is supported by the Southampton NIHR Biomedical Research Centre and the Southampton NIHR Clinical Research Facility, which are funded by the NIHR and are a partnership between the University of Southampton and University Hospital Southampton NHS Foundation Trust. Conceptualization, R.J.K. S.H.A. P.V. and G.S.; project lead, S.H.-D.L.M. C.R.-S. H.M. R.J.K. S.H.A. P.V. and G.S.; methodology and sample and clinical data collection, H.M. M.A.K. L.L. C.B. R.J.K. S.H.A. and G.S.; resources, H.M. R.J.K. S.H.A. P.V. and G.S.; performed experiments, S.H.-D.L.M. H.M. and G.S.; performed sequencing library preparation and sequencing runs, S.H.-D.L.M. H.S. S.L. and M.M.; performed computational and bioinformatics analysis, C.R.-S. and F.E.C.-C.; performed statistical analysis, S.H.-M. C.R.-S. and H.Z.; unrestricted access to data, S.H.-D.L.M. C.R.-S. H.M. F.E.C.-C. S.H.A. R.J.K. P.V. and G.S.; data analysis and interpretation, S.H.-D.L.M. C.R.-S. H.M. F.E.C.-C. P.V. and G.S.; writing and editing of manuscript, S.H.-D.L.M. C.R.-S. H.M. P.V. and G.S.; review of manuscript, all authors; funding and supervision, R.J.K. S.H.A. P.V. and G.S. All authors read and approved the final article and take responsibility for its content. S.H.A. received research funding unrelated to this work from Dyson Inc. We worked to ensure gender balance in the recruitment of human subjects. We worked to ensure that the study questionnaires were prepared in an inclusive way. One or more of the authors of this paper self-identifies as an underrepresented ethnic minority in their field of research or within their geographical location. One or more of the authors of this paper self-identifies as a gender minority in their field of research. One or more of the authors of this paper self-identifies as a member of the LGBTQIA+ community. We support inclusive, diverse, and equitable conduct of research. Funding Information: We thank Dr. C. Kim, Dr. D. Hinz, members of the flow cytometry core facility at La Jolla Institute for Immunology, and the members of the Vijayanand laboratory, for scientific support. We also thank all donors for their charitable contribution to academic research. This work was supported by NIH research grants R01HL114093 and U19-AI070535 (P.V.) and equipment grants ( S10RR027366 - BD FACSAria II , and S10OD025052 - Illumina Novaseq 6000); the William K. Bowes, Jr. Foundation (P.V.); and BioLegend – BioLegend Fellow (S.H.-M.). The WATCH study is supported by the Southampton NIHR Biomedical Research Centre and the Southampton NIHR Clinical Research Facility , which are funded by the NIHR and are a partnership between the University of Southampton and University Hospital Southampton NHS Foundation Trust. Publisher Copyright: © 2023 Elsevier Inc.

Keywords: CD4+ tissue-resident memory T cells, Translation to patients, airway T cells, asthma, cytotoxic CD4+ T cells, severe asthma, single-cell RNA sequencing

Local EPrints ID: 486157

URI: http://eprints.soton.ac.uk/id/eprint/486157

DOI: doi:10.1016/j.medj.2023.09.003

ISSN: 2666-6340

PURE UUID: baf6cfd6-0ecb-4f95-85b4-ce6f2b42ef7a

ORCID for Clair Barber: orcid.org/0000-0001-5335-5129

ORCID for Ramesh J. Kurukulaaratchy: orcid.org/0000-0002-1588-2400

Date deposited: 11 Jan 2024 17:48

Last modified: 18 Mar 2024 02:51