Ultra-sensitive immunoassays using multi-photon-detection in diagnostic proteomics of blood
Ultra-sensitive immunoassays using multi-photon-detection in diagnostic proteomics of blood
Multiphoton-detection methods that detect as little as 1000 atoms of 125I-streptavidin increase the sensitivity of immunoassays by 200- to 1000-fold (1 femtogram/mL). Improved background suppression allows 20- to 100-fold improvements in sensitivity for conventional immunoassays (10-50 femtogram/mL). Quantitation of low abundance biomarkers in blood (PSA, TNF, VEGF, IL-1, IL-6, and IL-8), for the first time for complete patient cohorts, indicates that very high analytical sensitivity and new statistical methods are crucial for serum-based diagnostic proteomics.
multiphoton-detection, blood, serum, proteomics, diagnostics, immunoassay, cytokines, growth factors, breast cancer, HIV-1
2375-2378
Drukier, A.K.
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Ossetrova, N.
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Schors, E.
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Brown, L.R.
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Tomaszewski, J.
99e38209-e215-4a2c-b912-8b95bd4429a9
Sainsbury, R.
f6c9f95c-7dda-48de-a4bc-3a7c00e09eef
Godovac-Zimmermann, J.
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2005
Drukier, A.K.
4d632450-ad5f-42e0-b031-54e31eb46d4c
Ossetrova, N.
645a9069-6784-4db1-b5cc-5750f49f39e0
Schors, E.
fe98fa1e-6241-4df2-abca-04737169397b
Brown, L.R.
11b49b4c-f161-41d5-81bc-15e84754e572
Tomaszewski, J.
99e38209-e215-4a2c-b912-8b95bd4429a9
Sainsbury, R.
f6c9f95c-7dda-48de-a4bc-3a7c00e09eef
Godovac-Zimmermann, J.
ea0ec1f8-691e-4d26-acdf-4fadcd907864
Drukier, A.K., Ossetrova, N., Schors, E., Brown, L.R., Tomaszewski, J., Sainsbury, R. and Godovac-Zimmermann, J.
(2005)
Ultra-sensitive immunoassays using multi-photon-detection in diagnostic proteomics of blood.
Journal of Proteome Research, 4 (6), .
(doi:10.1021/pr050320n).
Abstract
Multiphoton-detection methods that detect as little as 1000 atoms of 125I-streptavidin increase the sensitivity of immunoassays by 200- to 1000-fold (1 femtogram/mL). Improved background suppression allows 20- to 100-fold improvements in sensitivity for conventional immunoassays (10-50 femtogram/mL). Quantitation of low abundance biomarkers in blood (PSA, TNF, VEGF, IL-1, IL-6, and IL-8), for the first time for complete patient cohorts, indicates that very high analytical sensitivity and new statistical methods are crucial for serum-based diagnostic proteomics.
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Published date: 2005
Keywords:
multiphoton-detection, blood, serum, proteomics, diagnostics, immunoassay, cytokines, growth factors, breast cancer, HIV-1
Organisations:
Cancer Sciences
Identifiers
Local EPrints ID: 48630
URI: http://eprints.soton.ac.uk/id/eprint/48630
ISSN: 1535-3893
PURE UUID: 9e504245-ffe8-4589-a556-93f025288b0c
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Date deposited: 04 Oct 2007
Last modified: 15 Mar 2024 09:48
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Author:
A.K. Drukier
Author:
N. Ossetrova
Author:
E. Schors
Author:
L.R. Brown
Author:
J. Tomaszewski
Author:
R. Sainsbury
Author:
J. Godovac-Zimmermann
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