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Biological and mechanical response of graphene oxide surface-treated polylactic acid 3D-printed bone scaffolds: experimental and numerical approaches

Biological and mechanical response of graphene oxide surface-treated polylactic acid 3D-printed bone scaffolds: experimental and numerical approaches
Biological and mechanical response of graphene oxide surface-treated polylactic acid 3D-printed bone scaffolds: experimental and numerical approaches
Employing 3D printing bone scaffolds with various polymers is growing due to their biocompatibility, biodegradability, and good mechanical properties. However, their biological properties need modification to have fewer difficulties in clinical experiments. Herein, the fused-deposition modeling technique is used to design triply-periodic-minimal-surfaces polylactic-acid scaffolds and evaluate their biological response under static and dynamic cell culture conditions. To enhance the biological response of 3D-printed bone scaffolds, graphene-oxide (GO) is coated on the surface of the scaffolds. Fourier-transform infrared spectroscopy, X-ray diffraction, and energy-dispersion X-ray analysis are conducted to check the GO presence and its effects. Also, computational fluid dynamics analysis is implemented to investigate the shear stress on the scaffold, which is a critical parameter for cell proliferation under dynamic cell culture conditions. Compression tests and contact-angle measurements are performed to assess the GO effect on mechanical properties and wettability, respectively. Also, it was shown that surface-treated scaffolds have lower mechanical properties and higher wettability than uncoated scaffolds. A perfusion bioreactor is used to study cell culture. Also, field-emission-scanning-electron-microscope and 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl-tetrazolium-bromide (MTT) assay analyses are conducted to observe cell viability and cell attachment. An increase of up to 220% in viability was achieved with GO and dynamic cell culture.
3D printing, Bone scaffolds, Tissue engineering, bone scaffolds, tissue engineering, dynamic culture, mechanical properties
1438-1656
Keshtiban, Mohsen Mashhadi
4a24de4c-e2b3-412b-8385-4f5fafa538d1
Taghvaei, Hadi
5e4fadd6-dbb8-4565-9dd8-126fbfb62b99
Noroozi, Reza
9eed6adb-c1a2-40d2-945f-c3b07fd91c25
Eskandari, Vahid
ccb61d6b-bed7-46ec-b5f2-5734273423d6
Arif, Zia Ullah
49914102-f4f6-417f-9881-22a80015dedf
Bodaghi, Mahdi
3f0e3afe-4253-4a53-847b-03fb4e85bf14
Bardania, Hassan
e4fbc35d-20da-4dc9-8d79-17045570ff65
Hadi, Amin
94d6c973-2218-40c0-ba49-bff670152285
Keshtiban, Mohsen Mashhadi
4a24de4c-e2b3-412b-8385-4f5fafa538d1
Taghvaei, Hadi
5e4fadd6-dbb8-4565-9dd8-126fbfb62b99
Noroozi, Reza
9eed6adb-c1a2-40d2-945f-c3b07fd91c25
Eskandari, Vahid
ccb61d6b-bed7-46ec-b5f2-5734273423d6
Arif, Zia Ullah
49914102-f4f6-417f-9881-22a80015dedf
Bodaghi, Mahdi
3f0e3afe-4253-4a53-847b-03fb4e85bf14
Bardania, Hassan
e4fbc35d-20da-4dc9-8d79-17045570ff65
Hadi, Amin
94d6c973-2218-40c0-ba49-bff670152285

Keshtiban, Mohsen Mashhadi, Taghvaei, Hadi, Noroozi, Reza, Eskandari, Vahid, Arif, Zia Ullah, Bodaghi, Mahdi, Bardania, Hassan and Hadi, Amin (2024) Biological and mechanical response of graphene oxide surface-treated polylactic acid 3D-printed bone scaffolds: experimental and numerical approaches. Advanced Engineering Materials, 26 (3), [2301260]. (doi:10.1002/adem.202301260).

Record type: Article

Abstract

Employing 3D printing bone scaffolds with various polymers is growing due to their biocompatibility, biodegradability, and good mechanical properties. However, their biological properties need modification to have fewer difficulties in clinical experiments. Herein, the fused-deposition modeling technique is used to design triply-periodic-minimal-surfaces polylactic-acid scaffolds and evaluate their biological response under static and dynamic cell culture conditions. To enhance the biological response of 3D-printed bone scaffolds, graphene-oxide (GO) is coated on the surface of the scaffolds. Fourier-transform infrared spectroscopy, X-ray diffraction, and energy-dispersion X-ray analysis are conducted to check the GO presence and its effects. Also, computational fluid dynamics analysis is implemented to investigate the shear stress on the scaffold, which is a critical parameter for cell proliferation under dynamic cell culture conditions. Compression tests and contact-angle measurements are performed to assess the GO effect on mechanical properties and wettability, respectively. Also, it was shown that surface-treated scaffolds have lower mechanical properties and higher wettability than uncoated scaffolds. A perfusion bioreactor is used to study cell culture. Also, field-emission-scanning-electron-microscope and 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl-tetrazolium-bromide (MTT) assay analyses are conducted to observe cell viability and cell attachment. An increase of up to 220% in viability was achieved with GO and dynamic cell culture.

Text
Tissue_porous_scaffolds_Accepted_Version - Accepted Manuscript
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More information

Accepted/In Press date: 2024
e-pub ahead of print date: 11 January 2024
Published date: February 2024
Keywords: 3D printing, Bone scaffolds, Tissue engineering, bone scaffolds, tissue engineering, dynamic culture, mechanical properties

Identifiers

Local EPrints ID: 486529
URI: http://eprints.soton.ac.uk/id/eprint/486529
ISSN: 1438-1656
PURE UUID: 9af5f583-f7ee-4656-a718-7d6623d02fd6
ORCID for Zia Ullah Arif: ORCID iD orcid.org/0000-0002-9254-7606

Catalogue record

Date deposited: 25 Jan 2024 17:33
Last modified: 06 Jun 2024 02:18

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Contributors

Author: Mohsen Mashhadi Keshtiban
Author: Hadi Taghvaei
Author: Reza Noroozi
Author: Vahid Eskandari
Author: Zia Ullah Arif ORCID iD
Author: Mahdi Bodaghi
Author: Hassan Bardania
Author: Amin Hadi

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