A genomic island in Vibrio cholerae with VPI-1 site-specific recombination characteristics contains CRISPR-Cas and type VI secretion modules
A genomic island in Vibrio cholerae with VPI-1 site-specific recombination characteristics contains CRISPR-Cas and type VI secretion modules
Cholera is a devastating diarrhoeal disease caused by certain strains of serogroup O1/O139 Vibrio cholerae. Mobile genetic elements such as genomic islands (GIs) have been pivotal in the evolution of O1/O139 V. cholerae. Perhaps the most important GI involved in cholera disease is the V. cholerae pathogenicity island 1 (VPI-1). This GI contains the toxin-coregulated pilus (TCP) gene cluster that is necessary for colonization of the human intestine as well as being the receptor for infection by the cholera-toxin bearing CTX phage. In this study, we report a GI (designated GIVchS12) from a non-O1/O139 strain of V. cholerae that is present in the same chromosomal location as VPI-1, contains an integrase gene with 94% nucleotide and 100% protein identity to the VPI-1 integrase, and attachment (att) sites 100% identical to those found in VPI-1. However, instead of TCP and the other accessory genes present in VPI-1, GIVchS12 contains a CRISPR-Cas element and a type VI secretion system (T6SS). GIs similar to GIVchS12 were identified in other V. cholerae genomes, also containing CRISPR-Cas elements and/or T6SS's. This study highlights the diversity of GIs circulating in natural V. cholerae populations and identifies GIs with VPI-1 recombination characteristics as a propagator of CRISPR-Cas and T6SS modules.
Labbate, Maurizio
033d5ac8-a659-47ff-a5dc-79e99fa46ea4
Orata, Fabini D.
ff42f0a4-b279-496f-b293-26ca2675db89
Petty, Nicola K.
d0d0e7df-b1fe-4f75-8fcc-7f6731173c77
Jayatilleke, Nathasha D.
19e0244b-819b-4ba1-8bf5-ea50ff4a1d73
King, William L.
0bd4328a-34ba-4b9a-bf4e-1442c18c43fc
Kirchberger, Paul C.
fb5b5ea5-83e9-4ab3-9897-e13f1555e9de
Allen, Chris
fe643d61-fa8e-4237-a557-1657b9ea6855
Mann, Gulay
9df30d33-b43b-4487-bcca-6c609dd0a870
Mutreja, Ankur
182e0512-30b6-4cfb-82cb-e429b395e70b
Thomson, Nicholas R.
5497a110-069d-4156-bccb-c77db572b1c2
Boucher, Yan
49bcbdbb-f639-4c98-8885-4c6e631d8fdb
Charles, Ian G.
ec1bba08-d092-471e-8a8f-8dcdcb258d24
1 November 2016
Labbate, Maurizio
033d5ac8-a659-47ff-a5dc-79e99fa46ea4
Orata, Fabini D.
ff42f0a4-b279-496f-b293-26ca2675db89
Petty, Nicola K.
d0d0e7df-b1fe-4f75-8fcc-7f6731173c77
Jayatilleke, Nathasha D.
19e0244b-819b-4ba1-8bf5-ea50ff4a1d73
King, William L.
0bd4328a-34ba-4b9a-bf4e-1442c18c43fc
Kirchberger, Paul C.
fb5b5ea5-83e9-4ab3-9897-e13f1555e9de
Allen, Chris
fe643d61-fa8e-4237-a557-1657b9ea6855
Mann, Gulay
9df30d33-b43b-4487-bcca-6c609dd0a870
Mutreja, Ankur
182e0512-30b6-4cfb-82cb-e429b395e70b
Thomson, Nicholas R.
5497a110-069d-4156-bccb-c77db572b1c2
Boucher, Yan
49bcbdbb-f639-4c98-8885-4c6e631d8fdb
Charles, Ian G.
ec1bba08-d092-471e-8a8f-8dcdcb258d24
Labbate, Maurizio, Orata, Fabini D., Petty, Nicola K., Jayatilleke, Nathasha D., King, William L., Kirchberger, Paul C., Allen, Chris, Mann, Gulay, Mutreja, Ankur, Thomson, Nicholas R., Boucher, Yan and Charles, Ian G.
(2016)
A genomic island in Vibrio cholerae with VPI-1 site-specific recombination characteristics contains CRISPR-Cas and type VI secretion modules.
Scientific Reports, 6, [36891].
(doi:10.1038/srep36891).
Abstract
Cholera is a devastating diarrhoeal disease caused by certain strains of serogroup O1/O139 Vibrio cholerae. Mobile genetic elements such as genomic islands (GIs) have been pivotal in the evolution of O1/O139 V. cholerae. Perhaps the most important GI involved in cholera disease is the V. cholerae pathogenicity island 1 (VPI-1). This GI contains the toxin-coregulated pilus (TCP) gene cluster that is necessary for colonization of the human intestine as well as being the receptor for infection by the cholera-toxin bearing CTX phage. In this study, we report a GI (designated GIVchS12) from a non-O1/O139 strain of V. cholerae that is present in the same chromosomal location as VPI-1, contains an integrase gene with 94% nucleotide and 100% protein identity to the VPI-1 integrase, and attachment (att) sites 100% identical to those found in VPI-1. However, instead of TCP and the other accessory genes present in VPI-1, GIVchS12 contains a CRISPR-Cas element and a type VI secretion system (T6SS). GIs similar to GIVchS12 were identified in other V. cholerae genomes, also containing CRISPR-Cas elements and/or T6SS's. This study highlights the diversity of GIs circulating in natural V. cholerae populations and identifies GIs with VPI-1 recombination characteristics as a propagator of CRISPR-Cas and T6SS modules.
This record has no associated files available for download.
More information
Accepted/In Press date: 10 October 2016
Published date: 1 November 2016
Additional Information:
Publisher Copyright:
© 2016 The Author(s).
Identifiers
Local EPrints ID: 486613
URI: http://eprints.soton.ac.uk/id/eprint/486613
ISSN: 2045-2322
PURE UUID: b517b943-73a3-4f06-b815-5855c5c16744
Catalogue record
Date deposited: 26 Jan 2024 18:01
Last modified: 18 Mar 2024 04:18
Export record
Altmetrics
Contributors
Author:
Maurizio Labbate
Author:
Fabini D. Orata
Author:
Nicola K. Petty
Author:
Nathasha D. Jayatilleke
Author:
William L. King
Author:
Paul C. Kirchberger
Author:
Chris Allen
Author:
Gulay Mann
Author:
Ankur Mutreja
Author:
Nicholas R. Thomson
Author:
Yan Boucher
Author:
Ian G. Charles
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics