READ ME File For 'Dataset in support of the University of Southampton Doctoral Thesis 'Understanding and improving the mechanisms of action of anti-GD2 monoclonal antibody therapy in neuroblastoma''

Dataset DOI: https://doi.org/10.5258/SOTON/D2936

ReadMe Author: Tia Kulanthaivadivel, University of Southampton ORCID iD 0009-0008-1019-6621


This dataset supports the thesis entitled Understanding and improving the mechanisms of action of anti-GD2 monoclonal antibody therapy in neuroblastoma'
AWARDED BY: Univeristy of Southampton
DATE OF AWARD: 2024


This dataset contains:

This dataset contains experimental data collected from the CCI (University of Southampton) or 
NDCN (University of Oxford) laboratory. These datasets were collected and calculated by the researcher and 
analysed using GraphPad Prism Software. Biochemical, in vitro and in vivo experimental protocols 
were utilised to generate the data. 


The main aim was to characterise a panel of human and mouse anti-GD2 antibody Fc variants: 
human IgG1 wildtype; human IgG1K322A; human IgG1N297A; human IgG1PG-LALA; human IgG1P331G; human IgG1DLE; human IgG2; human IgG4; mouse IgG2a; mouse IgG2aK322A; mouse IgG2aN297A; mouse IgG2aPG-LALA; mouse IgG1, using in vitro and in vivo techniques. 

Biochemical analysis
SPR, ELISA and flow cytometric analysis was performed to assess the impact of the Fc domain modifications on target
protein (GD2) and effector ligand binding affinity (C1q and FcγRs). 


In vitro
Tumour and neuronal cell lines were used to characterise the effector functions of hu14.18 antibodies in 
complement dependent cytotoxicity, antibody-dependent cellular cytotoxicity, antibody-dependent cellular 
phagocytosis, MTT assay, CellTox Green assay, neurotoxicity assay, immunostaining and calcium imaging.   


In vivo
Wildtype and FcγRs knock out tumour mouse models were utilised to characterise the therapeutic efficacy and toxicity of the hu14.18 antibody variants. 


Date of data collection: 
Data was collected between October 2019 to December 2022


Information about geographic location of data collection: 
All data were generated in Southampton and Oxford UK.


Licence: CC BY

Date that the file was created: January 2024