READ ME File For Dataset supporting the University of Southampton Doctoral Thesis "Functional and structural establishment of a novel cyclic di-GMP signalling pathway in pseudomonas aeruginosa" Dataset DOI: 10.5258/SOTON/D2959 ReadMe Author: Zhi-xiu Yan, University of Southampton ORCID ID 0000-0003-1893-6221 This dataset supports the thesis entitled AWARDED BY: University of Southampton DATE OF AWARD: 14 Feb 2024 DESCRIPTION OF THE DATA This dataset includes eight compressed files. Data were originally collected from functional and structural biology experiments, including phenotypic assay data, PA4395 STD assay data, PA4395 backbone assignment data, PA4395 and cyclic di-GMP titration data, PA4395 and PA2692 titration data, as well as other essential data for this project accomplishment. Softwares including GraphPad Prism9, CCPNMR V3, Pymol, and Microsoft Office were required for opening of these data. All these data was collected and analysed independently by creator herself and supervised by supervisors without other participants. This dataset contains: Figures_for_chapters.zip phenotypic_data.zip STD_data.zip backbone_assignment.zip PA4395_cdgmp_titration_spectra.zip trNOESY_data.zip PA4395-PA2692_HSQC_titrations.zip other_data.zip Date of data collection: 2019.9.27-2023.9.26 Information about geographic location of data collection: Licence: CC-BY Related projects/Funders: China Scholarship Council National Biofilms innovation centre Date that the file was created: Feb, 2024