READ ME File For 'Dataset title' Dataset DOI:  10.5258/SOTON/D2984 ReadMe Author: Marcus Alexander Widdess, University of Southampton, 0000-0002-2063-8090 This dataset supports the thesis entitled "Multivalent and bispecific immunostimulatory antibodies for cancer immunotherapy" AWARDED BY: Univeristy of Southampton DATE OF AWARD: 2024 DESCRIPTION OF THE DATA Data include word, excel and powerpoint files of summary data and raw data, as well as fcs files of flow cytometry data, wsp files of flowjo analysis and .prism files from graphpad prism. Data is organised into thesis chapters and then experiment. fcs files were collected using either a BD FACS Calibur or a BD FACS Canto II flow cytometer and require flow cytometry analysis software, such as flowjo or fcs express to analyse. .wsp files require flowjo to be able to access the analysis. .prism files require Graphpad Prism V10 or later to open. This dataset contains: Data from my thesis chapters 3, 4 and 5, as well as associated work performed in the PCU. Data is broken down into thesis chapters: Chapter 3 - 1 folder, Chapter 4 - 2 folders and Chapter 5 - 1 folder. Contained within these folders is the in vitro work associated with the thesis, organised into seperate experiments. The PCU - thesis folder contains any PCU related experiments that were described in my thesis. These are organised into a folder per experiment with a brief experimental description. Date of data collection: 01/01/2019 to 31/07/2023 Information about geographic location of data collection: Data collection occured at Centre for Cancer Immunology, Coxford Road Southampton General Hospital Centre for Cancer Immunology (MP127, Southampton SO16 6YD Licence: CC-BY Related projects/Funders: Cancer Research UK Date that the file was created: February 2024