Krause, Robert, Ogongo, Paul, Tezera, Liku, Ahmed, Mohammed, Mbano, Ian, Chambers, Mark, Ngoepe, Abigail, Magnoumba, Magalli, Muema, Daniel, Karim, Farina, Khan, Khadija, Lumamba, Kapongo, Nargan, Kievershen, Madansein, Rajhmun, Steyn, Adrie, Shalek, Alex K., Elkington, Paul and Leslie, Al (2024) B cell heterogeneity in human tuberculosis highlights compartment-specific phenotype and functional roles. Communications Biology.
Abstract
B cells are important in tuberculosis (TB) immunity, but their role in the human lung is understudied. Here, we characterize B cells from lung tissue and matched blood of TB patients and found they are decreased in the blood and increased in the lungs, consistent with recruitment to infected tissue, where they are located in granuloma associated lymphoid tissue (GrALT). Flow cytometry and transcriptomics identified multiple B cell populations in the lung, including those associated with tissue resident memory, germinal centers, antibody secretion, proinflammatory atypical B cells, and regulatory B cells, some of which are expanded in TB disease. Additionally, TB lungs contained high levels of Mtb-reactive antibodies, specifically IgM, which promoted Mtb phagocytosis. Overall, these data reveal the presence of functionally diverse B cell subsets in TB diseased lung and suggest several potential localized roles that may represent a target for interventions to promote immunity or mitigate immunopathology.
More information
Identifiers
Catalogue record
Export record
Contributors
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.