READ ME File For 'Next Generation Sequencing Data SX5 screens HCT116 and MTAP -/- cells' and 'Next Generation Sequencing Data SX5 screens HCT116 and MTAP -/- cells'

Dataset DOI: 10.5258/SOTON/D3008

ReadMe Author: Monika Papayova, University of Southampton 0009-0006-9043-5017

This dataset supports the thesis entitled Development and Optimisation of High Throughput Screening Systems in Mammalian Cells 
AWARDED BY: Univeristy of Southampton
DATE OF AWARD: 2024

DESCRIPTION OF THE DATA

This dataset is embargoed until 5/3/27 after this it will be available 'on request' please complet the request form at 

This dataset contains:

The data related to the PhD thesis consists of 6 compressed (zip) files containing genetic sequences in FASTQ format. These files are as follows:
 
HCT116_10rep_fastq.zip - DNA sequences detected in the intein and extein region of HCT116 cells, after screening the SX5 library in these cells. 10 repeats are concatenated in a single file, (2GBytes)
MTAP_10rep_fastq.zip - DNA sequences detected in the intein and extein region of MTAP-/- cells, after screening the SX5 library in these cells. 10 repeats concatenated in a single file (1.8GBytes)

HCT116_SFX4_3rep_fastq.zip - DNA sequences detected in the intein and extein region of HCT116 cells, after screening the SFX4 library in these cells. 3 repeats concatenated in a single file, (0.5GBytes)
MTAP_SFX4_3rep_fastq.zip - DNA sequences detected in the intein and extein region of MTAP-/- cells, after screening the SFX4 library in these cells. 3 repeats concatenated in a single file (0.4GBytes)

sx5_hypoxia.zip - DNA sequences detected in the intein and extein region of TRex293 cells incubated in normoxia and hypoxia, after screening the SX5 library in these cells. There are 6 repeats concatenated in a single file (1.7GBytes)
sfx4_hypoxia_3rep.zip - DNA sequences detected in the intein and extein region of TRex293 cells incubated in normoxia and hypoxia, after screening the SFX4 library in these cells. There are 3 repeats concatenated in a single file (1.4GBytes)

The data was generated by sequencing genomic DNA collected from the relevant screens. The sequencing was carried out by an Illumina MiSeq sequencer and an Oxford Nanopore Sequencer.

Date of data collection: May 2022 - August 2024

Information about geographic location of data collection: Data collected at the University of Southampton, Building 30, Department of Chemistry. 

Licence:CC-BY-NC-ND 

Related projects/Funders: EPSRC

Related publication:

Date that the file was created: May 2022 - August 2024