Automation of fluorescence-based PCR for confirmation of meningococcal disease
Automation of fluorescence-based PCR for confirmation of meningococcal disease
A fluorescence-based PCR method was developed, fully automated, and used to confirm infection with Neisseria meningitidis by detection of the meningococcus-specific ctrA gene. The method provided a highly sensitive, high-throughput assay that was reproducible and less labor-intensive than manual methods.
Bacterial Proteins/genetics, DNA-Binding Proteins, Fluorescence, Humans, Meningococcal Infections/diagnosis, Neisseria meningitidis/genetics, Polymerase Chain Reaction/instrumentation, Robotics, Transcription Factors
4518-9
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Edwards, G F
f98fa562-77bd-45a3-827b-7a3f8dc76cdd
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
December 2001
Diggle, M A
d739c25b-e038-4a9a-850d-2e2fd6ff4a4f
Edwards, G F
f98fa562-77bd-45a3-827b-7a3f8dc76cdd
Clarke, S C
f7d7f7a2-4b1f-4b36-883a-0f967e73fb17
Diggle, M A, Edwards, G F and Clarke, S C
(2001)
Automation of fluorescence-based PCR for confirmation of meningococcal disease.
Journal of Clinical Microbiology, 39 (12), .
(doi:10.1128/JCM.39.12.4518-4519.2001).
Abstract
A fluorescence-based PCR method was developed, fully automated, and used to confirm infection with Neisseria meningitidis by detection of the meningococcus-specific ctrA gene. The method provided a highly sensitive, high-throughput assay that was reproducible and less labor-intensive than manual methods.
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Published date: December 2001
Keywords:
Bacterial Proteins/genetics, DNA-Binding Proteins, Fluorescence, Humans, Meningococcal Infections/diagnosis, Neisseria meningitidis/genetics, Polymerase Chain Reaction/instrumentation, Robotics, Transcription Factors
Identifiers
Local EPrints ID: 488127
URI: http://eprints.soton.ac.uk/id/eprint/488127
ISSN: 0095-1137
PURE UUID: 14a06b71-73f6-4606-9150-2cd5f19cfb27
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Date deposited: 16 Mar 2024 15:35
Last modified: 17 Mar 2024 03:07
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Author:
M A Diggle
Author:
G F Edwards
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