READ ME file for 'Dataset for the effect of colour on Marine Biofilm viscoelasticity" which is Chapter 7 of the Thesis. Developing artificial biofilms for investigating the effects of physico-mechanical properties on marine biofilm-associated drag Awarded 2023 Dataset DOI: https://doi.org/10.5258/SOTON/D2671 File was created: June 2023 ------------------- GENERAL INFORMATION ------------------- ReadMe Author: Alexandra Snowdon (formerly Jackson), University of Southampton [ORCID ID, 0000-0001-6672-937X] Date of data collection: October-December 2022 Information about geographic location of data collection: Data was collected and processed at two sites: Newcastle University Dove Laboratory (Cullercoats, UK) and AkzoNobel (Gateshead, UK) 1) Hartlepool Marina Marine Biofilms grown in-field on black square pipes 2) AkzoNobel Preparing and painting coupons (red, white and black) Optical Coherence Tomogrpaphy (OCT) - structural data of marine biofilms Parallel-plate Rheometer - rheological characterisation of marine biofilms Digital microscope for qualitative analysis This work was funded by a DTP ESPRC grant EP/R513325/1 to the University of Southampton with partial funding AkzoNobel. -------------------------- SHARING/ACCESS INFORMATION -------------------------- Licenses/restrictions placed on the data, or limitations of reuse: CC-BY -------------------- DATA & FILE OVERVIEW -------------------- This dataset contains: Each file refers to sructural and mechanical data collected for three different types of coloured coupon (4cm dia.): red, white and black. Each excel file has a README tab incorporated but briefly: 1) Pretest_BLI.xlsx - The surface roughness data for the three different coloured surfaces: red, white and black. 2) Pretest_OCA.xlsx - The contact angle data for the three different coloured surfaces: red, white and black. Rheometer experiments, gap height controlled by normal force (0.1 N). 3) 7+8_YieldStressData.xlsx - Amplitude sweeps for different coloured coupons were ran at 1 Hz and oscillatory strain was incrementally increased from 10-2 to 10^3. Data from 7 weeks and 8 weeks of fouling is shown here and an average and SD is calculated for each colour. Yield stress was taken as the intercept of G' and G''. 4) 7+8_StructuralOCTData.xlsx - Structural data for marine biofilms collected using Optical Coherency Tomography (OCT) before and after testing on the rheometer. Mean thickness (mm), maximum thickness (mm), roughness coefficient (Ra*) and percent coverage (%) were calculated using custom MATLAB scripts. In the workbook raw data is presented as well as the mean +/- SD. The percent differences between the before and after data are also reported. 5) 7weeks_AmplitudeSweeps.xlsx - Amplitude sweeps for different coloured coupons were ran at 1 Hz and oscillatory strain was incrementally increased from 10-2 to 10^3. Raw data data for biofilms developed over 7-weeks is shown here and an average and SD is calculated for each colour. 6) 7weeks_CreepRecoveryData.xlsx - Creep-recovery experiments for three different surfaces were ran at different stress values, ranging from 0.5 Pa - 4 Pa. Stress was applied for 120 seconds and then removed so that recovery could be measured. Shear modulus (G), viscosity (n) and elastic relaxation time were calculated from the creep-recovery curves using equations proposed in Gloag et al., 2018. 7) 7weeks_CreepRecoveryData_1Pa.xlsx - The same as above but shows raw data for creep-recovery at 1Pa. 8) 7weeks_FrequencySweeps.xlsx - Frequency sweeps were performed for three different surfaces after 7 weeks of fouling. Experiments were ran at an oscillation frequency of 0.1 Hz to 10Hz, which equated to an angular frequency of 0.62 to 62 rad s-1. The tests were held at a constant strain of 0.1%, which was within the LVR identified in the amplitude sweep data. Raw data is presented as well as the mean +/- SD. 9) 8weeks_AmplitudeSweeps.xlsx - Amplitude sweeps for different coloured coupons were ran at 1 Hz and oscillatory strain was incrementally increased from 10-2 to 10^3. Raw data data for biofilms developed over 8-weeks is shown here and an average and SD is calculated for each colour. 10) 8weeks_CreepRecoveryData.xlsx - Creep-recovery experiments for three different surfaces were ran at different stress values, ranging from 0.5 Pa - 4 Pa. Stress was applied for 120 seconds and then removed so that recovery could be measured. Shear modulus (G), viscosity (n) and elastic relaxation time were calculated from the creep-recovery curves using equations proposed in Gloag et al., 2018. 11) 8weeks_CreepRecoveryData_1Pa.xlsx - The same as above but shows raw data for creep-recovery at 1Pa. 12) 8weeks_FrequencySweeps.xlsx - Frequency sweeps were performed for three different surfaces after 8 weeks of fouling. Experiments were ran at an oscillation frequency of 0.1 Hz to 10Hz, which equated to an angular frequency of 0.62 to 62 rad s-1. The tests were held at a constant strain of 0.1%, which was within the LVR identified in the amplitude sweep data. Raw data is presented as well as the mean +/- SD.