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Effects of calcium peroxide pretreatment on the substrate, microenvironment, and microbial community in an anaerobic digestion system of waste sludge

Effects of calcium peroxide pretreatment on the substrate, microenvironment, and microbial community in an anaerobic digestion system of waste sludge
Effects of calcium peroxide pretreatment on the substrate, microenvironment, and microbial community in an anaerobic digestion system of waste sludge
In this study, the waste activated sludge (WAS) of wastewater treatment plants was pretreated with calcium peroxide (CP) for 48 h and then digested anaerobically at 35 ±1 ◦C. The effects of CP pretreatment on the substrate, microenvironment, and microbial community in the anaerobic digestion (AD) system of WAS were investigated. Results showed that the total volatile fatty acids in the pretreated sludge increased by 8.95 times compared with that in the control. Evidently, pH and oxidation reduction potential (ORP) increased when the CP-pretreated sludge was further subjected to AD, where NO3 N and H2O2 reached 29.65 mg/L and 11.8 mg/L, respectively, but the catalase (CAT) activity was inhibited. Additionally, CP-pretreatment activated the nitrifying bacterial phylum, inhibited the denitrifying genera, and selected the specific methanogens. However, CH4 production was still enhanced by 19.10 % unexpectedly although higher ORP was maintained in the AD system for a long time, which challenged the classical theory of AD. The acceptable feedback of the sludge AD system on CP pretreatment may be related to the change in H2O2 and CAT activities.
Calcium peroxide pretreatment, Hydrogen peroxide, Methanogenic bacteria select, Nitrate accumulation, Sludge anaerobic digestion
2214-7144
Wang, Yi
e7c00360-9f7d-4ad3-8ac9-af7a11e26868
Yin, Jia-Ni
8f91e49e-ec38-439e-8b05-a45cf02a445e
Li, Xia
b1864b73-b5f9-458d-b731-b7c9763e3286
Liu, Yong-Qiang
75adc6f8-aa83-484e-9e87-6c8442e344fa
Wang, Yi
e7c00360-9f7d-4ad3-8ac9-af7a11e26868
Yin, Jia-Ni
8f91e49e-ec38-439e-8b05-a45cf02a445e
Li, Xia
b1864b73-b5f9-458d-b731-b7c9763e3286
Liu, Yong-Qiang
75adc6f8-aa83-484e-9e87-6c8442e344fa

Wang, Yi, Yin, Jia-Ni, Li, Xia and Liu, Yong-Qiang (2024) Effects of calcium peroxide pretreatment on the substrate, microenvironment, and microbial community in an anaerobic digestion system of waste sludge. Journal of Water Process Engineering, 59, [104985]. (doi:10.1016/j.jwpe.2024.104985).

Record type: Article

Abstract

In this study, the waste activated sludge (WAS) of wastewater treatment plants was pretreated with calcium peroxide (CP) for 48 h and then digested anaerobically at 35 ±1 ◦C. The effects of CP pretreatment on the substrate, microenvironment, and microbial community in the anaerobic digestion (AD) system of WAS were investigated. Results showed that the total volatile fatty acids in the pretreated sludge increased by 8.95 times compared with that in the control. Evidently, pH and oxidation reduction potential (ORP) increased when the CP-pretreated sludge was further subjected to AD, where NO3 N and H2O2 reached 29.65 mg/L and 11.8 mg/L, respectively, but the catalase (CAT) activity was inhibited. Additionally, CP-pretreatment activated the nitrifying bacterial phylum, inhibited the denitrifying genera, and selected the specific methanogens. However, CH4 production was still enhanced by 19.10 % unexpectedly although higher ORP was maintained in the AD system for a long time, which challenged the classical theory of AD. The acceptable feedback of the sludge AD system on CP pretreatment may be related to the change in H2O2 and CAT activities.

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Accepted/In Press date: 7 February 2024
Published date: 15 February 2024
Keywords: Calcium peroxide pretreatment, Hydrogen peroxide, Methanogenic bacteria select, Nitrate accumulation, Sludge anaerobic digestion

Identifiers

Local EPrints ID: 488926
URI: http://eprints.soton.ac.uk/id/eprint/488926
ISSN: 2214-7144
PURE UUID: 3a7bcbd3-8f6e-4095-a9b7-225df860051e
ORCID for Yong-Qiang Liu: ORCID iD orcid.org/0000-0001-9688-1786

Catalogue record

Date deposited: 09 Apr 2024 16:50
Last modified: 22 May 2024 01:44

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Contributors

Author: Yi Wang
Author: Jia-Ni Yin
Author: Xia Li
Author: Yong-Qiang Liu ORCID iD

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