

READ ME File For ‘Cytotoxic responses’

Dataset DOI: 10.5258/SOTON/D2892

ReadMe Author: Sahar Farag, University of Southampton ORCID ID 0000-0003-2360-7094

This dataset supports the thesis entitled ‘The functional effect of CD1c responsive T cells in human Tuberculosis (TB)’
AWARDED BY: University of Southampton
DATE OF AWARD: 2024

DESCRIPTION OF THE DATA

This dataset contains:
‘Figure 40_CD1c MFI’ – The expression of CD1c on THP1-CD1c cells infected with a range of MOIs of TB was analysed using flow cytometry. MFI values were obtained using FlowJo. 
‘Figure 41_Cytotoxicity at 24 and 48h’ – ToxiLight data of cytotoxicity caused by P1C5 and P3D7 T cell lines following co-culture with uninfected and infected THP1-CD1c cells at 24h and 48h. 
‘Figure 42A&C_Specificity of P1C5 T cells’, ‘Figure 42_B_Specificity of P1C5 T cells’ – ToxiLight data of cytotoxicity caused by P1C5 and P3D7 T cell lines following co-culture with uninfected and infected THP1 cells lines.
‘Figure 42A&C_Statistical analysis’ and ‘Figure 42_B_Statistical analysis’ - Statistical analysis of ToxiLight data presented in Figure 42.
‘Figure 42D_Specificity of P1C5 T cells’ - ToxiLight data of cytotoxicity caused by P1C5 T cell lines following co-culture with uninfected and infected THP1-CD1c with a range of MOI of UV-killed TB.
‘Figure 43_Cytotoxic T cell dose response’ – ToxiLight data of cytotoxicity caused by increasing ratios of P1C5 and P3D7 T cell lines following co-culture with infected THP1-KO and THP1-CD1c cells lines (figure A), and infected or uninfected THP1-CD1c cells (figure B).
‘Figure 43_Statistical analysis’ – Statistical analysis of ToxiLight data presented in Figure 43.
‘Figure 44A_MOI dose response & statistical analysis’- ToxiLight data of cytotoxicity caused by P1C5 T cell lines following co-culture with THP1-KO and THP1-CD1c cells infected with increasing MOI of UV-killed TB. Statistical analysis of ToxiLight data presented in Figure 44A also included.
‘Figure 44B_MOI dose response P1C5 vs P3D7’- ToxiLight data of cytotoxicity caused by P1C5 and P3D7 T cell lines following co-culture with uninfected or infected THP1-CD1c cells with increasing MOI of UV-killed TB.
‘Figure 44B_Statistical analysis’ – Statistical analysis of ToxiLight data presented in Figure 44B.
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Date of data collection: April 2022 – June 2022

Information about geographic location of data collection: Southampton General Hospital, Southampton, UK.

Licence:
CC-BY

Date that the file was created: April 2024

