READ ME File For 'Dataset supporting the University of Southampton Doctoral Thesis "Endometriosis – It’s Influence on Obstetric and Birth Outcomes and the Potential Role of Reactive Oxygen Species"'

Dataset DOI: 10.5258/SOTON/D3025

ReadMe Author: Joanne Horton, University of Southampton

This dataset supports the thesis entitled "Endometriosis – It’s Influence on Obstetric and Birth Outcomes and the Potential Role of Reactive Oxygen Species"
AWARDED BY: Univeristy of Southampton
DATE OF AWARD: 2024

DESCRIPTION OF THE DATA

5 xls files 
TAC assay results dataset
TBARS assay results dataset
ROS assay dataset
roGFP results dataset
Immunofluorescence results dataset
2 word files
Patient consent form for study XSESS
Patient information sheet for study XSESS

This dataset contains:
TAC assay results dataset: Raw data of total antioxidant capacity assay results. TAC assay applied to samples of peritoneal fluid, urine and serum of women with and without endometriosis taken at surgery and serum and urine obtained at follow up as part of XSESS study (ethically approved) and analysis formed part of above named thesis. Dataset includes the raw data assay results by anonymised patient study number denoted "XS..." and lists of XS samples by control vs disease status, reason for surgery, pathology found at surgery and severity of disease by ASRM stage. 

TBARS assay results dataset: Raw data of Thiobarbituric acid reactive substances assay results. TBARS assay applied to samples of peritoneal fluid, urine and serum of women with and without endometriosis taken at surgery and serum and urine obtained at follow up as part of XSESS study (ethically approved) and analysis formed part of above named thesis. Dataset includes the raw data assay results by anonymised patient study number denoted "XS..." and lists of XS samples by control vs disease status, reason for surgery, pathology found at surgery and severity of disease by ASRM stage. 

ROS assay dataset: Raw data of Lumigen total reactive oxygen species assay results. ROS assay applied to samples of peritoneal fluid, urine and serum of women with and without endometriosis taken at surgery and serum and urine obtained at follow up as part of XSESS study (ethically approved) and analysis formed part of above named thesis. Dataset includes the raw data assay results by anonymised patient study number denoted "XS..." and lists of XS samples by control vs disease status, reason for surgery, pathology found at surgery and severity of disease by ASRM stage. 

roGFP results dataset: Raw data results of intracellular redox status experiments. Microinjection of mouse oocytes with roGFP2 mRNA and measured fluorescence (mean integrated density of confocal images) of the oocytes at emission wavelength 530 nm following excitation at 405 nm and 488 nm wavelengths to measure the oxidation/reduction ratio of each oocyte when incubated in peritoneal fluid of women with and without endometriosis from XSESS study and control fluids. 

Immunofluorescence results dataset: Raw data results of experiments incubating mouse oocytes in peritoneal fluid (of women with and without endometriosis from XSESS study) and control fluids and performing immunofluorescence with Anti-8-OHG antibody. Mean fluorescence of anti-8-OHG was measured by integrated density in pixels.


Date of data collection: 2017-2020

Information about geographic location of data collection: Southampton, UK

Licence:
Data is available only 'on request' to bone fide researchers with ethical approval. Please complete the attached request form and return it to researchdata@soton.ac.uk 

Related projects/Funders:
Funded by the Clinical Obstetric and Gynaecology division of the Human Development and Health department at the University of Southampton



Date that the file was created: April, 2024