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The monoclonal antibody HB1 recognizes an adhesion molecule for macrophages in the brain

The monoclonal antibody HB1 recognizes an adhesion molecule for macrophages in the brain
The monoclonal antibody HB1 recognizes an adhesion molecule for macrophages in the brain

The brain environment exerts a powerful influence on macrophage phenotype, as exemplified by microglia, but the mechanisms mediating this control are nuclear. Since adhesion molecules are known to transmit signals across cell membranes, we investigated adhesion receptors involved in macrophage interaction with brain tissue. We have demonstrated previously that macrophages adhere specifically to CNS neurones in an in vitro assay. Here we show that this adhesion is inhibited by lectins, including Griffonia simplicofolia isolectin B4 (GSI), which has been used as a microglial marker for many years. Adhesion is unaffected by antibodies to several known adhesion molecules but is markedly inhibited by a new monoclonal antibody: HB1. HB1 recognizes microglia in the normal brain and activated microglia and recruited monocytes during CNS pathology. It labels a subset of resident macrophages and recruited monocytes in other tissues. Using this antibody, we isolated a protein of about 110 kDa from macrophage cell lysates. This protein is recognized by GSI, providing the first evidence of a functional role for the antigen labelled by this lectin. Further study of the HB1 antigen may provide important information about the influence of the brain environment on the phenotype of monocytic cells.

0300-4864
867-876
Brown, Heidi
b962757e-55c9-4f13-b603-203cdd1e4278
Townsend, Mandy
2c631395-a90e-4f3b-aa54-2712a1807faa
Fearn, Sara
b3099e59-4b18-4e3a-80ab-a5cc7e17a57b
Perry, Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
Brown, Heidi
b962757e-55c9-4f13-b603-203cdd1e4278
Townsend, Mandy
2c631395-a90e-4f3b-aa54-2712a1807faa
Fearn, Sara
b3099e59-4b18-4e3a-80ab-a5cc7e17a57b
Perry, Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4

Brown, Heidi, Townsend, Mandy, Fearn, Sara and Perry, Hugh (1998) The monoclonal antibody HB1 recognizes an adhesion molecule for macrophages in the brain. Journal of Neurocytology, 27 (12), 867-876. (doi:10.1023/A:1006932505819).

Record type: Article

Abstract

The brain environment exerts a powerful influence on macrophage phenotype, as exemplified by microglia, but the mechanisms mediating this control are nuclear. Since adhesion molecules are known to transmit signals across cell membranes, we investigated adhesion receptors involved in macrophage interaction with brain tissue. We have demonstrated previously that macrophages adhere specifically to CNS neurones in an in vitro assay. Here we show that this adhesion is inhibited by lectins, including Griffonia simplicofolia isolectin B4 (GSI), which has been used as a microglial marker for many years. Adhesion is unaffected by antibodies to several known adhesion molecules but is markedly inhibited by a new monoclonal antibody: HB1. HB1 recognizes microglia in the normal brain and activated microglia and recruited monocytes during CNS pathology. It labels a subset of resident macrophages and recruited monocytes in other tissues. Using this antibody, we isolated a protein of about 110 kDa from macrophage cell lysates. This protein is recognized by GSI, providing the first evidence of a functional role for the antigen labelled by this lectin. Further study of the HB1 antigen may provide important information about the influence of the brain environment on the phenotype of monocytic cells.

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Published date: December 1998

Identifiers

Local EPrints ID: 489320
URI: http://eprints.soton.ac.uk/id/eprint/489320
ISSN: 0300-4864
PURE UUID: fabc2f83-a306-4f5f-b339-90bf67ca6b8e

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Date deposited: 19 Apr 2024 16:55
Last modified: 05 Jun 2024 17:19

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Contributors

Author: Heidi Brown
Author: Mandy Townsend
Author: Sara Fearn
Author: Hugh Perry

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