In vivo cellular phosphatidylcholine kinetics of CD15+ leucocytes and CD3+ T-lymphocytes in adults with acute respiratory distress syndrome
In vivo cellular phosphatidylcholine kinetics of CD15+ leucocytes and CD3+ T-lymphocytes in adults with acute respiratory distress syndrome
Mammalian cell membranes composed of a mixture of glycerophospholipids, the relative composition of individual phospholipids and the dynamic flux vary between cells. In addition to their structural role, membrane phospholipids are involved in cellular signalling and immunomodulatory functions. In this study, we investigate the molecular membrane composition and dynamic flux of phosphatidylcholines in CD15+ leucocytes and CD3+ lymphocytes extracted from patients with acute respiratory distress syndrome (ARDS). We identified compositional variations between these cell types, where CD15+ cells had relatively higher quantities of alkyl-acyl PC species and CD3+ cells contained more arachidonoyl-PC species. There was a significant loss of arachidonoyl-PC in CD3+ cells in ARDS patients. Moreover, there were significant changes in PC composition and the methyl-D9 enrichment of individual molecular species in CD15+ cells from ARDS patients. This is the first study to perform an in vivo assessment of membrane composition and dynamic changes in immunological cells from ARDS patients.
ARDS, lymphocyte, methyl-D-choline, neutrophil, phosphatidylcholine, phospholipids
Dushianthan, Ahilanandan
013692a2-cf26-4278-80bd-9d8fcdb17751
Cusack, Rebecca
dfb1595f-2792-4f76-ac6d-da027cf40146
Goss, Victoria
ef02be5d-9318-4f7d-b076-3153555980d0
Koster, Grielof
e404c38a-6f48-430a-adf0-5208228cb9e7
Grocott, Michael P.W.
1e87b741-513e-4a22-be13-0f7bb344e8c2
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
11 February 2024
Dushianthan, Ahilanandan
013692a2-cf26-4278-80bd-9d8fcdb17751
Cusack, Rebecca
dfb1595f-2792-4f76-ac6d-da027cf40146
Goss, Victoria
ef02be5d-9318-4f7d-b076-3153555980d0
Koster, Grielof
e404c38a-6f48-430a-adf0-5208228cb9e7
Grocott, Michael P.W.
1e87b741-513e-4a22-be13-0f7bb344e8c2
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Dushianthan, Ahilanandan, Cusack, Rebecca, Goss, Victoria, Koster, Grielof, Grocott, Michael P.W. and Postle, Anthony D.
(2024)
In vivo cellular phosphatidylcholine kinetics of CD15+ leucocytes and CD3+ T-lymphocytes in adults with acute respiratory distress syndrome.
Cells, 13 (4), [332].
(doi:10.3390/cells13040332).
Abstract
Mammalian cell membranes composed of a mixture of glycerophospholipids, the relative composition of individual phospholipids and the dynamic flux vary between cells. In addition to their structural role, membrane phospholipids are involved in cellular signalling and immunomodulatory functions. In this study, we investigate the molecular membrane composition and dynamic flux of phosphatidylcholines in CD15+ leucocytes and CD3+ lymphocytes extracted from patients with acute respiratory distress syndrome (ARDS). We identified compositional variations between these cell types, where CD15+ cells had relatively higher quantities of alkyl-acyl PC species and CD3+ cells contained more arachidonoyl-PC species. There was a significant loss of arachidonoyl-PC in CD3+ cells in ARDS patients. Moreover, there were significant changes in PC composition and the methyl-D9 enrichment of individual molecular species in CD15+ cells from ARDS patients. This is the first study to perform an in vivo assessment of membrane composition and dynamic changes in immunological cells from ARDS patients.
Text
cells-13-00332
- Version of Record
More information
Accepted/In Press date: 9 February 2024
Published date: 11 February 2024
Additional Information:
Publisher Copyright:
© 2024 by the authors.
Keywords:
ARDS, lymphocyte, methyl-D-choline, neutrophil, phosphatidylcholine, phospholipids
Identifiers
Local EPrints ID: 490282
URI: http://eprints.soton.ac.uk/id/eprint/490282
ISSN: 2073-4409
PURE UUID: 90c8fdc8-5a0b-4834-b680-87f222ce061c
Catalogue record
Date deposited: 23 May 2024 16:36
Last modified: 06 Jun 2024 02:03
Export record
Altmetrics
Contributors
Author:
Ahilanandan Dushianthan
Author:
Rebecca Cusack
Author:
Victoria Goss
Author:
Grielof Koster
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics