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In silico mechanics of stem cells intramyocardially transplanted with a biomaterial injectate for treatment of myocardial infarction

In silico mechanics of stem cells intramyocardially transplanted with a biomaterial injectate for treatment of myocardial infarction
In silico mechanics of stem cells intramyocardially transplanted with a biomaterial injectate for treatment of myocardial infarction

Purpose: Biomaterial and stem cell delivery are promising approaches to treating myocardial infarction. However, the mechanical and biochemical mechanisms underlying the therapeutic benefits require further clarification. This study aimed to assess the deformation of stem cells injected with the biomaterial into the infarcted heart. Methods: A microstructural finite element model of a mid-wall infarcted myocardial region was developed from ex vivo microcomputed tomography data of a rat heart with left ventricular infarct and intramyocardial biomaterial injectate. Nine cells were numerically seeded in the injectate of the microstructural model. The microstructural and a previously developed biventricular finite element model of the same rat heart were used to quantify the deformation of the cells during a cardiac cycle for a biomaterial elastic modulus (E inj) ranging between 4.1 and 405,900 kPa. Results: The transplanted cells’ deformation was largest for E inj = 7.4 kPa, matching that of the cells, and decreased for an increase and decrease in E inj. The cell deformation was more sensitive to E inj changes for softer (E inj ≤ 738 kPa) than stiffer biomaterials. Conclusions: Combining the microstructural and biventricular finite element models enables quantifying micromechanics of transplanted cells in the heart. The approach offers a broader scope for in silico investigations of biomaterial and cell therapies for myocardial infarction and other cardiac pathologies.

Biomaterial injection therapy, Cell mechanics, Cell therapy, Finite element method
1869-408X
Motchon, Y.D.
70b2c775-6bf0-43ff-a943-1eefd79ff283
Sack, K.L.
ecdad0f1-5231-42f7-97d0-d62fc88fc446
Sirry, M.S.
703f8989-9b27-4805-a490-7a2114d13cc7
Nchejane, N.J.
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Abdalrahman, T.
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Nagawa, J.
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Kruger, M.
94f2a6d7-90b7-4b1e-8a30-988fc7da88df
Pauwels, E
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Van Loo, D
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De Muynck, A
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Van Hoorebeke, L
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Davies, N.H.
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Franz, Thomas
31f508f4-6851-4274-b256-cc01ab321d50
Motchon, Y.D.
70b2c775-6bf0-43ff-a943-1eefd79ff283
Sack, K.L.
ecdad0f1-5231-42f7-97d0-d62fc88fc446
Sirry, M.S.
703f8989-9b27-4805-a490-7a2114d13cc7
Nchejane, N.J.
a50096e2-4f38-48e9-94c9-77d57e4340b4
Abdalrahman, T.
0fb146d9-fb94-4f9e-a8b1-fe7d9ba88eda
Nagawa, J.
32553fa1-e3ca-4917-89d9-e8164b3eb05b
Kruger, M.
94f2a6d7-90b7-4b1e-8a30-988fc7da88df
Pauwels, E
40fb5ba4-fc51-4ac3-ac19-c785b770ef19
Van Loo, D
2f46edd3-2f3b-4586-9c0d-44f5e1fb7573
De Muynck, A
2c29d7ed-9597-4aee-abcd-7a691a0ff2d9
Van Hoorebeke, L
2fa0d76d-cf4d-4cf1-a7e3-91d596daf06f
Davies, N.H.
35b3a3b0-2903-414c-9cf4-47fe944de1b1
Franz, Thomas
31f508f4-6851-4274-b256-cc01ab321d50

Motchon, Y.D., Sack, K.L., Sirry, M.S., Nchejane, N.J., Abdalrahman, T., Nagawa, J., Kruger, M., Pauwels, E, Van Loo, D, De Muynck, A, Van Hoorebeke, L, Davies, N.H. and Franz, Thomas (2024) In silico mechanics of stem cells intramyocardially transplanted with a biomaterial injectate for treatment of myocardial infarction. Cardiovascular Engineering and Technology. (doi:10.1007/s13239-024-00734-1).

Record type: Article

Abstract

Purpose: Biomaterial and stem cell delivery are promising approaches to treating myocardial infarction. However, the mechanical and biochemical mechanisms underlying the therapeutic benefits require further clarification. This study aimed to assess the deformation of stem cells injected with the biomaterial into the infarcted heart. Methods: A microstructural finite element model of a mid-wall infarcted myocardial region was developed from ex vivo microcomputed tomography data of a rat heart with left ventricular infarct and intramyocardial biomaterial injectate. Nine cells were numerically seeded in the injectate of the microstructural model. The microstructural and a previously developed biventricular finite element model of the same rat heart were used to quantify the deformation of the cells during a cardiac cycle for a biomaterial elastic modulus (E inj) ranging between 4.1 and 405,900 kPa. Results: The transplanted cells’ deformation was largest for E inj = 7.4 kPa, matching that of the cells, and decreased for an increase and decrease in E inj. The cell deformation was more sensitive to E inj changes for softer (E inj ≤ 738 kPa) than stiffer biomaterials. Conclusions: Combining the microstructural and biventricular finite element models enables quantifying micromechanics of transplanted cells in the heart. The approach offers a broader scope for in silico investigations of biomaterial and cell therapies for myocardial infarction and other cardiac pathologies.

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More information

Accepted/In Press date: 12 May 2024
e-pub ahead of print date: 23 May 2024
Additional Information: Publisher Copyright: © The Author(s) 2024.
Keywords: Biomaterial injection therapy, Cell mechanics, Cell therapy, Finite element method

Identifiers

Local EPrints ID: 490715
URI: http://eprints.soton.ac.uk/id/eprint/490715
ISSN: 1869-408X
PURE UUID: 66a1e74c-1a81-4241-b85c-6177e6c926b2

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Date deposited: 04 Jun 2024 16:37
Last modified: 17 Jun 2024 17:05

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Contributors

Author: Y.D. Motchon
Author: K.L. Sack
Author: M.S. Sirry
Author: N.J. Nchejane
Author: T. Abdalrahman
Author: J. Nagawa
Author: M. Kruger
Author: E Pauwels
Author: D Van Loo
Author: A De Muynck
Author: L Van Hoorebeke
Author: N.H. Davies
Author: Thomas Franz

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