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Impaired reproduction, energy reserves and dysbiosis: The overlooked consequences of heatwaves in a bivalve mollusc

Impaired reproduction, energy reserves and dysbiosis: The overlooked consequences of heatwaves in a bivalve mollusc
Impaired reproduction, energy reserves and dysbiosis: The overlooked consequences of heatwaves in a bivalve mollusc
Extreme events like Marine Heatwaves (MHWs) are becoming more intense, severe, and frequent, threatening benthic communities, specifically bivalves. However, the consequences of non-lethal MHWs on animals are still poorly understood. Here, we exposed the Manila clam Ruditapes philippinarum to non-lethal MHW for 30 days and provided an integrative view of its effects. Our result indicated that albeit non-lethal, MHW reduced clam's energy reserves (by reducing their hepato-somatic index), triggered antioxidant defenses (particularly in males), impaired reproduction (via the production of smaller oocytes in females), triggered dysbiosis in the digestive gland microbiota and altered animals' behaviour (by impacting their burying capacity) and filtration rate. Such effects were seen also at RNA-seq (i.e. many down-regulated genes belonged to reproduction) and metabolome level. Interestingly, negative effects were more pronounced in males than in females. Our results show that MHWs influence animal physiology at multiple levels, likely impacting its fitness and its ecosystem services.
0025-326X
Peruzza, Luca
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Tucci, Carmen Federica
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Frizzo, Riccardo
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Riello, Tobia
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Quagliariello, Andrea
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Martino, Maria Elena
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Manuzzi, Alice
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Rovere, Giulia Dalla
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Bonsembiante, Federico
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Gelain, Maria Elena
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Smits, Morgan
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Borgheresi, Oliviero
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Camerani, Francesco
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Panin, Mattia
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Venier, Paola
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Mammi, Stefano
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Hauton, Chris
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Patarnello, Tomaso
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Milan, Massimo
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Bargelloni, Luca
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Peruzza, Luca
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Tucci, Carmen Federica
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Frizzo, Riccardo
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Riello, Tobia
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Quagliariello, Andrea
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Martino, Maria Elena
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Manuzzi, Alice
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Rovere, Giulia Dalla
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Bonsembiante, Federico
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Gelain, Maria Elena
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Smits, Morgan
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Borgheresi, Oliviero
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Camerani, Francesco
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Panin, Mattia
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Venier, Paola
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Mammi, Stefano
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Hauton, Chris
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Patarnello, Tomaso
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Milan, Massimo
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Bargelloni, Luca
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Peruzza, Luca, Tucci, Carmen Federica, Frizzo, Riccardo, Riello, Tobia, Quagliariello, Andrea, Martino, Maria Elena, Manuzzi, Alice, Rovere, Giulia Dalla, Bonsembiante, Federico, Gelain, Maria Elena, Smits, Morgan, Borgheresi, Oliviero, Camerani, Francesco, Panin, Mattia, Venier, Paola, Mammi, Stefano, Hauton, Chris, Patarnello, Tomaso, Milan, Massimo and Bargelloni, Luca (2023) Impaired reproduction, energy reserves and dysbiosis: The overlooked consequences of heatwaves in a bivalve mollusc. Marine Pollution Bulletin, 193, [115192]. (doi:10.1016/j.marpolbul.2023.115192).

Record type: Article

Abstract

Extreme events like Marine Heatwaves (MHWs) are becoming more intense, severe, and frequent, threatening benthic communities, specifically bivalves. However, the consequences of non-lethal MHWs on animals are still poorly understood. Here, we exposed the Manila clam Ruditapes philippinarum to non-lethal MHW for 30 days and provided an integrative view of its effects. Our result indicated that albeit non-lethal, MHW reduced clam's energy reserves (by reducing their hepato-somatic index), triggered antioxidant defenses (particularly in males), impaired reproduction (via the production of smaller oocytes in females), triggered dysbiosis in the digestive gland microbiota and altered animals' behaviour (by impacting their burying capacity) and filtration rate. Such effects were seen also at RNA-seq (i.e. many down-regulated genes belonged to reproduction) and metabolome level. Interestingly, negative effects were more pronounced in males than in females. Our results show that MHWs influence animal physiology at multiple levels, likely impacting its fitness and its ecosystem services.

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Accepted/In Press date: 15 June 2023
e-pub ahead of print date: 24 June 2023
Published date: 24 June 2023

Identifiers

Local EPrints ID: 490908
URI: http://eprints.soton.ac.uk/id/eprint/490908
ISSN: 0025-326X
PURE UUID: 0e5ccd57-82af-4dd7-be80-e1a1db9dc21d
ORCID for Chris Hauton: ORCID iD orcid.org/0000-0002-2313-4226

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Date deposited: 07 Jun 2024 17:49
Last modified: 08 Jun 2024 01:35

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Contributors

Author: Luca Peruzza
Author: Carmen Federica Tucci
Author: Riccardo Frizzo
Author: Tobia Riello
Author: Andrea Quagliariello
Author: Maria Elena Martino
Author: Alice Manuzzi
Author: Giulia Dalla Rovere
Author: Federico Bonsembiante
Author: Maria Elena Gelain
Author: Morgan Smits
Author: Oliviero Borgheresi
Author: Francesco Camerani
Author: Mattia Panin
Author: Paola Venier
Author: Stefano Mammi
Author: Chris Hauton ORCID iD
Author: Tomaso Patarnello
Author: Massimo Milan
Author: Luca Bargelloni

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