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In vitro bactericidal activity of biogenic copper oxide nanoparticles for Neisseria gonorrhoeae with enhanced compatibility for human cells

In vitro bactericidal activity of biogenic copper oxide nanoparticles for Neisseria gonorrhoeae with enhanced compatibility for human cells
In vitro bactericidal activity of biogenic copper oxide nanoparticles for Neisseria gonorrhoeae with enhanced compatibility for human cells

Resistance to antibiotics and antimicrobial compounds is a significant problem for human and animal health globally. The development and introduction of new antimicrobial compounds are urgently needed, and copper oxide nanoparticles (CuO NPs) have found widespread application across various sectors including biomedicine, pharmacy, catalysis, cosmetics, and many others. What makes them particularly attractive is the possibility of their synthesis through biogenic routes. In this study, we synthesized biogenic green tea (GT, Camellia sinensis)-derived CuO NPs (GT CuO NPs) and examined their biophysical properties, in vitro toxicity for mammalian cells in culture, and then tested them against Neisseria gonorrhoeae, an exemplar Gram-negative bacterium from the World Health Organization’s Priority Pathogen List. We compared our synthesized GT CuOP NPs with commercial CuO NPs (Com CuO NPs). Com CuO NPs were significantly more cytotoxic to mammalian cells (IC50 of 7.32 μg/mL) than GT CuO NPs (IC50 of 106.1 μg/mL). GT CuO NPs showed no significant increase in bax, bcl2, il6, and il1β mRNA expression from mammalian cells, whereas there were notable rises after treatment with Com CuO NPs. GT-CuO NPs required concentrations of 0.625 and 3.125 μg/mL to kill 50 and 100% of bacteria, respectively, whereas Com-CuO NPs needed concentrations of 15.625 and 30 μg/mL to kill 50 and 100% of bacteria, and the antibiotic ceftriaxone killed 50 and 100% with 3.125 and 30 μg/mL. Gonococci could be killed within 30 min of exposure to GT CuO NPs and the NPs could kill up to 10 7 within 1 h. In summary, this is the first report to our knowledge that describes the bioactivity of biogenic CuO NPs against N. gonorrhoeae. Our data suggest that biogenic nanoparticle synthesis has significant advantages over traditional chemical routes of synthesis and highlights the potential of GT-CuO NPs in addressing the challenges posed by multidrug-resistant Neisseria gonorrhoeae infections.

Neisseria gonorrhoeae, antimicrobial agents, biogenic nanoparticles, copper, cytotoxicity
1944-8244
21633-21642
De Melo Santana, Bianca
d1b29455-b46c-4c41-8829-59d6c4b62943
Armentano, Giovana Marchini
330a21a2-39c2-4a09-9121-49185ee67731
Ferreira, Dayana Agnes Santos
12885d99-cdf9-4d95-88a6-14150e9e9a01
de Freitas, Camila Simões
1b78dbe8-efe1-4114-97cd-ea88c6d22766
Carneiro-Ramos, Marcela Sorelli
3df3a1cc-b431-4ebb-93f6-e5e8c269ffc4
Barozzi Seabra, Amedea
9d49fd6d-fc67-402e-932e-b3d40df94fde
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
De Melo Santana, Bianca
d1b29455-b46c-4c41-8829-59d6c4b62943
Armentano, Giovana Marchini
330a21a2-39c2-4a09-9121-49185ee67731
Ferreira, Dayana Agnes Santos
12885d99-cdf9-4d95-88a6-14150e9e9a01
de Freitas, Camila Simões
1b78dbe8-efe1-4114-97cd-ea88c6d22766
Carneiro-Ramos, Marcela Sorelli
3df3a1cc-b431-4ebb-93f6-e5e8c269ffc4
Barozzi Seabra, Amedea
9d49fd6d-fc67-402e-932e-b3d40df94fde
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078

De Melo Santana, Bianca, Armentano, Giovana Marchini, Ferreira, Dayana Agnes Santos, de Freitas, Camila Simões, Carneiro-Ramos, Marcela Sorelli, Barozzi Seabra, Amedea and Christodoulides, Myron (2024) In vitro bactericidal activity of biogenic copper oxide nanoparticles for Neisseria gonorrhoeae with enhanced compatibility for human cells. ACS Applied Materials and Interfaces, 16 (17), 21633-21642. (doi:10.1021/acsami.4c02357).

Record type: Article

Abstract

Resistance to antibiotics and antimicrobial compounds is a significant problem for human and animal health globally. The development and introduction of new antimicrobial compounds are urgently needed, and copper oxide nanoparticles (CuO NPs) have found widespread application across various sectors including biomedicine, pharmacy, catalysis, cosmetics, and many others. What makes them particularly attractive is the possibility of their synthesis through biogenic routes. In this study, we synthesized biogenic green tea (GT, Camellia sinensis)-derived CuO NPs (GT CuO NPs) and examined their biophysical properties, in vitro toxicity for mammalian cells in culture, and then tested them against Neisseria gonorrhoeae, an exemplar Gram-negative bacterium from the World Health Organization’s Priority Pathogen List. We compared our synthesized GT CuOP NPs with commercial CuO NPs (Com CuO NPs). Com CuO NPs were significantly more cytotoxic to mammalian cells (IC50 of 7.32 μg/mL) than GT CuO NPs (IC50 of 106.1 μg/mL). GT CuO NPs showed no significant increase in bax, bcl2, il6, and il1β mRNA expression from mammalian cells, whereas there were notable rises after treatment with Com CuO NPs. GT-CuO NPs required concentrations of 0.625 and 3.125 μg/mL to kill 50 and 100% of bacteria, respectively, whereas Com-CuO NPs needed concentrations of 15.625 and 30 μg/mL to kill 50 and 100% of bacteria, and the antibiotic ceftriaxone killed 50 and 100% with 3.125 and 30 μg/mL. Gonococci could be killed within 30 min of exposure to GT CuO NPs and the NPs could kill up to 10 7 within 1 h. In summary, this is the first report to our knowledge that describes the bioactivity of biogenic CuO NPs against N. gonorrhoeae. Our data suggest that biogenic nanoparticle synthesis has significant advantages over traditional chemical routes of synthesis and highlights the potential of GT-CuO NPs in addressing the challenges posed by multidrug-resistant Neisseria gonorrhoeae infections.

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Accepted/In Press date: 8 April 2024
e-pub ahead of print date: 18 April 2024
Published date: 1 May 2024
Keywords: Neisseria gonorrhoeae, antimicrobial agents, biogenic nanoparticles, copper, cytotoxicity

Identifiers

Local EPrints ID: 491245
URI: http://eprints.soton.ac.uk/id/eprint/491245
ISSN: 1944-8244
PURE UUID: 2aa26f4a-d749-4402-bff2-f48a68036795
ORCID for Myron Christodoulides: ORCID iD orcid.org/0000-0002-9663-4731

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Date deposited: 18 Jun 2024 16:42
Last modified: 22 Jun 2024 01:32

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Contributors

Author: Bianca De Melo Santana
Author: Giovana Marchini Armentano
Author: Dayana Agnes Santos Ferreira
Author: Camila Simões de Freitas
Author: Marcela Sorelli Carneiro-Ramos
Author: Amedea Barozzi Seabra

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