Mast cells in the urinary bladders of interstitial cystitis/bladder pain syndrome patients express the Mast cell Related G-Protein coupled Receptor X2 (MRGPRX2): potential role in the pathogenesis of neurogenic inflammation

Abdelwahab, Omar Kamal Abdelhalim (2021) Mast cells in the urinary bladders of interstitial cystitis/bladder pain syndrome patients express the Mast cell Related G-Protein coupled Receptor X2 (MRGPRX2): potential role in the pathogenesis of neurogenic inflammation. EAU21 Virtual Congress. 08 - 12 Jul 2021.

Abstract

Introduction & objectives: mast cells numbers and activity are significantly elevated in the bladders of patients with interstitial cystitis/bladder pain syndrome (IC/ BPS). This condition is associated with an increased density of the bladder sensory nerve endings which release neuroactive substances, e.g. substance P (SP), thereby inducing mast cell degranulation and tissue inflammation. However, the responsiveness of mast cells to SP is variable between different tissues depending on the local cellular micro-environment. MRGPRX2 is a recently identified G-protein coupled receptor involved in mast cell responsiveness to SP in different chronic inflammatory conditions, and its expression is associated with increased tissue inflammation. The responsiveness of the urinary bladder mast cells to SP has not yet been explored, and the potential contribution to neurogenic inflammation in the pathogenesis of BPS/IC deserves evaluation.

Materials & methods: in the current study, we investigated the responsiveness of bladder mast cells to the SP through studying the expression of MRGPRX2 on BPS/IC bladder mast cells. Urinary bladder biopsies, from 32 consented BPS/IC patients, were serially sectioned and stained with antibodies for the mast cell-specific proteases tryptase and chymase, in addition to MRGPRX2.

Results: 30 out of 32 BPS/IC biopsies consistently co-expressed MRGPRX2 as well as Tryptase and chymase, in the lamina propria and detrusor layers of the bladder wall, indicating positive responsiveness of the bladder mast cells to SP.

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Contributors

Author: Omar Kamal Abdelhalim Abdelwahab

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