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A microfluidic based system for analysis of single cells based on Ca2+ flux

Record type: Article

A microfluidic format-based system has been developed for in situ monitoring of the calcium flux response to agonists using Chinese hamster ovary (CHO) cells. The assay is based on measuring the fluorescent intensity of the calcium-sensitive indicator, Fluo-4 AM, and was performed in a modified glass chip channel, whose surface was functionalised using a silanisation method with 3-aminopropyltriethoxysilane (APTS) (enabling the cells to be immobilised on the channel surface). CHO cells calcium flux response was measured for different agonists over a range of concentrations. Cells and reagents were introduced into the chip in a continuous flow as a series of plugs in a given sequence.

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Citation

Zhang, Xunli, Yin, Huabing, Cooper, Jon M. and Haswell, Stephen J. (2006) A microfluidic based system for analysis of single cells based on Ca2+ flux Electrophoresis, 27, (24), pp. 5093-5100. (doi:10.1002/elps.200600390).

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Published date: November 2006

Identifiers

Local EPrints ID: 49139
URI: http://eprints.soton.ac.uk/id/eprint/49139
ISSN: 0173-0835
PURE UUID: 51162727-37da-42d3-ade8-2dcf3888e8a0

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Date deposited: 24 Oct 2007
Last modified: 17 Jul 2017 14:57

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Contributors

Author: Xunli Zhang
Author: Huabing Yin
Author: Jon M. Cooper
Author: Stephen J. Haswell

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