The University of Southampton
University of Southampton Institutional Repository

A microfluidic based system for analysis of single cells based on Ca2+ flux

Zhang, Xunli, Yin, Huabing, Cooper, Jon M. and Haswell, Stephen J. (2006) A microfluidic based system for analysis of single cells based on Ca2+ flux Electrophoresis, 27, (24), pp. 5093-5100. (doi:10.1002/elps.200600390).

Record type: Article


A microfluidic format-based system has been developed for in situ monitoring of the calcium flux response to agonists using Chinese hamster ovary (CHO) cells. The assay is based on measuring the fluorescent intensity of the calcium-sensitive indicator, Fluo-4 AM, and was performed in a modified glass chip channel, whose surface was functionalised using a silanisation method with 3-aminopropyltriethoxysilane (APTS) (enabling the cells to be immobilised on the channel surface). CHO cells calcium flux response was measured for different agonists over a range of concentrations. Cells and reagents were introduced into the chip in a continuous flow as a series of plugs in a given sequence.

PDF 49139-01.pdf - Other
Restricted to Repository staff only
Download (369kB)

More information

Published date: November 2006


Local EPrints ID: 49139
ISSN: 0173-0835
PURE UUID: 51162727-37da-42d3-ade8-2dcf3888e8a0

Catalogue record

Date deposited: 24 Oct 2007
Last modified: 17 Jul 2017 14:57

Export record



Author: Xunli Zhang
Author: Huabing Yin
Author: Jon M. Cooper
Author: Stephen J. Haswell

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.