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(Invited) Detection of D-serine using an enzymatic amperometric biosensor and its localized detection using scanning electrochemical microscopy

(Invited) Detection of D-serine using an enzymatic amperometric biosensor and its localized detection using scanning electrochemical microscopy
(Invited) Detection of D-serine using an enzymatic amperometric biosensor and its localized detection using scanning electrochemical microscopy
D-Serine acts as an endogenous co-agonist for N-methyl-D-aspartate receptors at synapses, making it essential for proper brain development and function. This amino acid has also been linked to several neurodegenerative diseases such as Alzheimer's disease and dementia. Nevertheless, the primary site and mechanism of D-serine release remains unclear. We recently demonstrated the use of an enzymatic amperometric biosensor for the in vivo quantification of endogenous D-serine release in Xenopus laevis tadpoles. We also investigate the effect of the permselective polymer layer thickness on the biosensor's response time and selectivity. Finally, scanning electrochemical microscopy (SECM) is then used with the optimized biosensor to measure localized release of D-serine from a model system. This SECM methodology, which provides high spatial and temporal resolution, could be useful to investigate the primary site and mechanism of D-serine release in other biological samples.
Polcari, David
c013e9f2-2cef-4281-8427-020d46cbdc80
Perry, Samuel C.
8e204d86-4a9c-4a5d-9932-cf470174648e
Pollegioni, Loredano
d0e713a9-0952-4f77-9b3e-8f61095e6e30
Geissler, Matthias
17173c36-94d5-42ff-95ea-1427801a7cc1
Mauzeroll, Janine
af84f034-1e52-4419-a1c2-ce7116db5b07
Polcari, David
c013e9f2-2cef-4281-8427-020d46cbdc80
Perry, Samuel C.
8e204d86-4a9c-4a5d-9932-cf470174648e
Pollegioni, Loredano
d0e713a9-0952-4f77-9b3e-8f61095e6e30
Geissler, Matthias
17173c36-94d5-42ff-95ea-1427801a7cc1
Mauzeroll, Janine
af84f034-1e52-4419-a1c2-ce7116db5b07

Polcari, David, Perry, Samuel C., Pollegioni, Loredano, Geissler, Matthias and Mauzeroll, Janine (2017) (Invited) Detection of D-serine using an enzymatic amperometric biosensor and its localized detection using scanning electrochemical microscopy. ECS Meeting Abstracts, MA2017-02, [2325]. (doi:10.1149/MA2017-02/55/2325).

Record type: Article

Abstract

D-Serine acts as an endogenous co-agonist for N-methyl-D-aspartate receptors at synapses, making it essential for proper brain development and function. This amino acid has also been linked to several neurodegenerative diseases such as Alzheimer's disease and dementia. Nevertheless, the primary site and mechanism of D-serine release remains unclear. We recently demonstrated the use of an enzymatic amperometric biosensor for the in vivo quantification of endogenous D-serine release in Xenopus laevis tadpoles. We also investigate the effect of the permselective polymer layer thickness on the biosensor's response time and selectivity. Finally, scanning electrochemical microscopy (SECM) is then used with the optimized biosensor to measure localized release of D-serine from a model system. This SECM methodology, which provides high spatial and temporal resolution, could be useful to investigate the primary site and mechanism of D-serine release in other biological samples.

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Published date: 1 September 2017

Identifiers

Local EPrints ID: 491837
URI: http://eprints.soton.ac.uk/id/eprint/491837
PURE UUID: a1216e4c-f694-4456-b9ee-7f4340a8b383
ORCID for Samuel C. Perry: ORCID iD orcid.org/0000-0002-6263-6114

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Date deposited: 04 Jul 2024 16:58
Last modified: 11 Jul 2024 02:01

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Contributors

Author: David Polcari
Author: Samuel C. Perry ORCID iD
Author: Loredano Pollegioni
Author: Matthias Geissler
Author: Janine Mauzeroll

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