READ ME File For 'Data supporting the publication "Single-cell high precision ablation using nanosecond-pulsed thulium-doped fiber laser"' Dataset DOI: 10.5258/SOTON/D3119 Date that the file was created: June, 2024 ------------------- GENERAL INFORMATION ------------------- ReadMe Author: Matthew D. Gerard, University of Southampton, Orcid ID: 0000-0002-9017-4068 Date of data collection: 2022-2023 Information about geographic location of data collection: Southampton, UK -------------------------- SHARING/ACCESS INFORMATION -------------------------- Licenses/restrictions placed on the data, or limitations of reuse: CC BY This dataset supports the publication: AUTHORS: Matthew D. Gerard, Hiroki Cook, James A. Read, Ibrahim H. Abughazaleh, Panuwat Srisamran, Siddhi Chugh, Sijing Liang, Qiang Fu, Richard O. C. Oreffo, David J. Richardson, Sumeet Mahajan, Lin Xu TITLE: Single-cell high precision ablation using nanosecond-pulsed thulium-doped fiber laser JOURNAL: Optical Engineering -------------------- DATA & FILE OVERVIEW -------------------- This dataset contains: Single cell paper Primary Dataset.xlsx The access to the data is embargoed to 14 august 2024 -------------------------- METHODOLOGICAL INFORMATION -------------------------- Description of methods used for collection/generation of data: The data in figure 3 (a) was measured using an Ophir power metre. The data in figure 3(b) was measured using an optical spectrum analyser (OSA). The data in figure 3 (c) was measured using a photodiode in conjunction with a Tektronix oscilloscope. The data in figure 3 (d) was measured with an infrared scanhead and a 75mm plano-convex lens, recording by how much the beam diverged as the scanhead moved a known perpendicular distance. The data was subsequently fitted to a hyperbolic curve, allowing for calculation of the M^2 value ofbeam quality. The data in figures 7 (a-d) corresponds to area measurements taken from high quality images of ablated tissue samples. The areas were measured using the selection brush imaging tool from the ImageJ software, wth pixel size translated to um^2 using appropriate microscopy scaling standards. The ablation area (AA) was defined as the fully denatured and vapourised area, and the thermally altered area (TAA) was defined as the area of irreversible thermal damage. The various other parameters were directly controlled using equipment in the system. The data in figures 12 (a-d) was measured in a similar fashion as the above paragraph. Only AA was measured due to structural constraints in the tissue sample. -------------------------------- METHODS FOR PROCESSING THE DATA: -------------------------------- The hyperbolic fits were done in Mathematica, and the area's were measured using ImageJ. No other software or processing was used. Software- or Instrument-specific information needed to interpret the data, including software and hardware version numbers: All that's needed to interpret the data is Microsoft Excel. It can be graphed and compared to the data in the manuscript. Environmental/experimental conditions: Air-conditioned lab at 293K. People involved with sample collection, processing, analysis and/or submission: Matthew D. Gerard, and Hiroki Cook -------------------------- DATA-SPECIFIC INFORMATION -------------------------- Number of variables: 15 Number of cases/rows: 16 Variable list, defining any abbreviations, units of measure, codes or symbols used: W - Watt, nm - nanometre, dBm - decibel milliwatt, s - second, V - Volt, mm - millimetre, um - micrometre, um^2 - micrometre squared, ns - nanosecond, MHz, megahertz AA - Ablation Area, TAA - Thermally Altered Area Missing data codes: Ablation Efficiency(can be calculated from the raw data provided).