The University of Southampton
University of Southampton Institutional Repository

Novel techniques for high-resolution functional imaging of trabecular bone

Novel techniques for high-resolution functional imaging of trabecular bone
Novel techniques for high-resolution functional imaging of trabecular bone
In current biological and biomedical research, quantitative endpoints have become an important factor of success. Classically, such endpoints were investigated with 2D imaging, which is usually destructive and the 3D character of tissue gets lost. 3D imaging has gained in importance as a tool for both, qualitative and quantitative assessment of biological systems. In this context synchrotron radiation based tomography has become a very effective tool for opaque 3D tissue systems. Results from a new device are presented enabling the 3D investigation of trabecular bone under mechanical load in a time-lapsed fashion. Using the highly brilliant X-rays from a synchrotron radiation source, bone microcracks and an indication for un-cracked ligament bridging are uncovered. 3D microcrack analysis proves that the classification of microcracks from 2D images is ambiguous. Fatigued bone was found to fail in burst-like fashion, whereas non-fatigued bone exhibited a distinct failure band. Additionally, a higher increase in microcrack volume was detected in fatigued in comparison to non-fatigued bone. Below the spatial resolution accessible with synchrotron radiation tomography we investigated native and fractured bone surfaces on the molecular scale with atomic force microscopy. The mineralized fibrils detected on fracture surfaces give rise to the assumption that the mineral-mineral interface is the weakest link in bone. The presented results show the power of functional micro-imaging, as well as the possibilities for AFM imaging (functional nano-imaging) in this context.
515-526
SPIE - The International Society for Optical Engineering
Thurner, Philipp J.
ab711ddd-784e-48de-aaad-f56aec40f84f
Müller, Ralph
f881853a-540f-48f1-bb6d-e0cf1894e036
Kindt, Johannes H.
e85fe547-4187-4ad9-9353-911a9bb84d9b
Schitter, Georg
a75aafc7-0804-4bbc-a2ee-792a17aed5fa
Fantner, Georg E.
e95e5469-568d-4c76-843c-bb8a4f6153df
Wyss, Peter
37835676-2f92-4df7-bc35-97b2c80c1104
Sennhauser, Urs
3c0e14aa-da0a-48da-bb7b-c65401b1d01b
Hansma, Paul K.
aeab95c4-0f23-4690-8302-72db3316215d
Amini, Amir A.
Manduca, Armando
Thurner, Philipp J.
ab711ddd-784e-48de-aaad-f56aec40f84f
Müller, Ralph
f881853a-540f-48f1-bb6d-e0cf1894e036
Kindt, Johannes H.
e85fe547-4187-4ad9-9353-911a9bb84d9b
Schitter, Georg
a75aafc7-0804-4bbc-a2ee-792a17aed5fa
Fantner, Georg E.
e95e5469-568d-4c76-843c-bb8a4f6153df
Wyss, Peter
37835676-2f92-4df7-bc35-97b2c80c1104
Sennhauser, Urs
3c0e14aa-da0a-48da-bb7b-c65401b1d01b
Hansma, Paul K.
aeab95c4-0f23-4690-8302-72db3316215d
Amini, Amir A.
Manduca, Armando

Thurner, Philipp J., Müller, Ralph, Kindt, Johannes H., Schitter, Georg, Fantner, Georg E., Wyss, Peter, Sennhauser, Urs and Hansma, Paul K. (2005) Novel techniques for high-resolution functional imaging of trabecular bone. Amini, Amir A. and Manduca, Armando (eds.) In Medical Imaging 2005: Physiology, Function, and Structure from Medical Images. vol. 5746, SPIE - The International Society for Optical Engineering. pp. 515-526 . (doi:10.1117/12.595799).

Record type: Conference or Workshop Item (Paper)

Abstract

In current biological and biomedical research, quantitative endpoints have become an important factor of success. Classically, such endpoints were investigated with 2D imaging, which is usually destructive and the 3D character of tissue gets lost. 3D imaging has gained in importance as a tool for both, qualitative and quantitative assessment of biological systems. In this context synchrotron radiation based tomography has become a very effective tool for opaque 3D tissue systems. Results from a new device are presented enabling the 3D investigation of trabecular bone under mechanical load in a time-lapsed fashion. Using the highly brilliant X-rays from a synchrotron radiation source, bone microcracks and an indication for un-cracked ligament bridging are uncovered. 3D microcrack analysis proves that the classification of microcracks from 2D images is ambiguous. Fatigued bone was found to fail in burst-like fashion, whereas non-fatigued bone exhibited a distinct failure band. Additionally, a higher increase in microcrack volume was detected in fatigued in comparison to non-fatigued bone. Below the spatial resolution accessible with synchrotron radiation tomography we investigated native and fractured bone surfaces on the molecular scale with atomic force microscopy. The mineralized fibrils detected on fracture surfaces give rise to the assumption that the mineral-mineral interface is the weakest link in bone. The presented results show the power of functional micro-imaging, as well as the possibilities for AFM imaging (functional nano-imaging) in this context.

This record has no associated files available for download.

More information

Published date: 13 March 2005
Venue - Dates: SPIE Medical Imaging, San Diego, USA, 2005-02-12 - 2005-02-12

Identifiers

Local EPrints ID: 49337
URI: http://eprints.soton.ac.uk/id/eprint/49337
PURE UUID: fec9d6a1-cdf1-4642-b151-92b65e18fada
ORCID for Philipp J. Thurner: ORCID iD orcid.org/0000-0001-7588-9041

Catalogue record

Date deposited: 30 Oct 2007
Last modified: 15 Mar 2024 09:55

Export record

Altmetrics

Contributors

Author: Ralph Müller
Author: Johannes H. Kindt
Author: Georg Schitter
Author: Georg E. Fantner
Author: Peter Wyss
Author: Urs Sennhauser
Author: Paul K. Hansma
Editor: Amir A. Amini
Editor: Armando Manduca

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×